M.R. Amorim1 F.R. Vargas, J.C. Llerena Junior, M.S. Pombo-de-Oliveira
Published August 27, 2007
Genet. Mol. Res. 6 (3): 500-503 (2007)

About the authors
M.R. Amorim1 F.R. Vargas, J.C. Llerena Junior, M.S. Pombo-de-Oliveira

Corresponding author: M.S. Pombo-de-Oliveira
E-mail: mpombo@inca.gov.br

ABSTRACT

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.

Key words: DNA extraction, DNAzol, Cell suspension, Cytogenetic