Luciana de Souza Ondei
Published: March 17, 2006
Genet. Mol. Res. 5 (1) : 88-88

Cite this Article:
Lde Souza Ondei (2006). Electrophoretic and chromatographic profile for“S-like” hemoglobin (Perfil eletroforético e cromatográfico das hemoglobinas “S-like”). Genet. Mol. Res. 5(1): 88-88.

About the Authors
Luciana de Souza Ondei

Hemoglobin variants originate mainly by simple amino acid substitutions, the result ofnucleotide sequence changes. Recently, the number of known abnormal hemoglobins has increased due to improvement in analysis methodologies; however, many laboratories are notprepared to correctly identify mutants. Hb S is a very well-characterized hemoglobin variantthat varies in prevalence in different regions of Brazil. However, there is a type of Hb thatpresents electrophoretic migration in alkaline pH similar to Hb S, named S-like Hb, which can beincorrectly diagnosed; therefore, its frequency is underestimated. We obtained reference rangesfor Hb S by HPLC, and we examined the electrophoretic and chromatographic profiles of S-likeHb. Hb Hasharon, Hb D-Los Angeles, Hb Korle-Bu, Hb Lepore, Hb D-Iran, Hb G-like, HbQueens, Hb Montgomery, and Hb Q-India were found. Cases of association between two betachain mutants were also found. Read More. . .

Key words: Hemoglobin variants, Laboratory diagnosis, Electrophoresis, HPLC.