Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Marcio José Poças-Fonseca, Marlene Teixeira De-Souza, Diorge Paulo de Souza, João Ricardo Moreira Almeida, Camila Marinho-Silva,  Nádia Skorupa Parachin, Alessandra da Silva Dantas Thiago Machado Mello-de-Sousa, Lídia Maria Pepe de Moraes
Published June 21, 2005
Genet. Mol. Res. 4 (2): 232-250 (2005)

About the Authors
Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Marcio José Poças-Fonseca, Marlene Teixeira De-Souza, Diorge Paulo de Souza, João Ricardo Moreira Almeida, Camila Marinho-Silva,  Nádia Skorupa Parachin, Alessandra da Silva Dantas Thiago Machado Mello-de-Sousa, Lídia Maria Pepe de Moraes

Corresponding author
L.M.P. de Moraes
Email: lmoraes@unb.br

ABSTRACT

DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understanding the life cycle of an organism. We used computational tools for data mining of genes involved in these processes in the pathogenic fungus Paracoccidiodes brasiliensis. Data derived from transcriptome analysis revealed that the cell cycle of this fungus, as well as DNA replication and repair, and the recombination machineries, are highly similar to those of the yeast Saccharomyces cerevisiae. Among orthologs detected in both species, there are genes related to cytoskeleton structure and assembly, chromosome segregation, and cell cycle control genes. We identified at least one representative gene from each step of the initiation of DNA replication. Major players in the process of DNA damage and repair were also identified.

Key words: Paracoccidioides brasiliensis, Transcriptome, Cell cycle, DNA replication, DNA repair, Recombination.