Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs

Melissa Nunes Miziara, Penny K. Riggs, M. Elisabete J. Amaral
Published: December 30, 2004
Genet. Mol. Res. 3 (4) : 465-473
 
Cite this Article:
M.Nunes Miziara, P.K. Riggs, E.J. Amaral (2004). Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs. Genet. Mol. Res. 3(4): 465-473.
 
About the Authors 
Melissa Nunes Miziara, Penny K. Riggs, M. Elisabete J. Amaral
 
Corresponding author
M.E.J. Amaral
E-mail: eamaral@bio.ibilce.unesp.br 
 
ABSTRACT

Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene transcriptional and posttranscriptional processes. A phylogenetic footprinting strategy was employed to identify noncoding conservation patterns of 39 human and bovine orthologous genes. Seventy-three conserved noncoding sequences were identified that shared greater than 70% identity over at least 100 bp. Thirteen of these conserved sequences were also identified in the mouse genome. Evolutionary conservation of noncoding sequences across diverse species may have functional significance, and these conserved sequences may be good candidates for regulatory elements.

Key words: Bovine, Genes, DNA sequences, Comparative analysis, Orthologous.

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