Cytogenetics of genetic counseling patients in Pelotas, Rio Grande do Sul, Brazil

A.C. Duarte, E. Cunha, J.M. Roth, G.L. Garcias, M.G. Martino-Roth, F. L.S. Ferreira
Published: July 16, 2004
Genet. Mol. Res. 3 (3) : 303-308
 
Cite this Article:
A.C. Duarte, E. Cunha, J.M. Roth, G.L. Garcias, M.G. Martino-Roth, F.L.S. Ferreira (2004). Cytogenetics of genetic counseling patients in Pelotas, Rio Grande do Sul, Brazil. Genet. Mol. Res. 3(3): 303-308.
 
About the Authors 
A.C. Duarte, E. Cunha, J.M. Roth, G.L. Garcias, M.G. Martino-Roth, F. L.S. Ferreira
 
Corresponding author
M.G. Martino-Roth
E-mail: martino@ufpel.tche.br 
 
ABSTRACT

From 1986 to 2002, we examined the chromosomal composition of 916 patients attended by two genetic counseling services in the city of Pelotas, in the Brazilian State of Rio Grande do Sul, to determine the genetic causes of their disturbances. Patterns of G-banding using trypsin and Giemsa (GTG) and C-banding using barium and Giemsa (CBG) were studied using phytohemagglutinin M-stimulated lymphocytes cultured from peripheral blood. Among the patients, 110 had Down’s syndrome, 7 had Edward’s syndrome, 4 had Patau’s syndrome, 29 had Turner’s syndrome, 5 had Klinefelter’s syndrome, and 3 had “cri-du-chat” syndrome. Abnormal chromosomes were observed in 29.3% of the patients. Most of these (56.3%) were numerical abnormalities, with the remaining being structural variants.

Key words: Chromosomal disorders, Genetic counseling.

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