Methodology

Related GMR Articles

06/28/2011
COI; Phylogenetic analysis; Tegillarca granosa

Blood cockles are among the most economically important brackish water invertebrates found in Malaysia. However, our knowledge of blood cockle phylogeny and systematics is rudimentary, especially for the species Tegillarca granosa. It is unclear, for instance, whether the cockles occurring on the west coast of peninsular Malaysia constitute a single species, or multiple, ... more

S.Y. Chee; M.N. Devakie; M.N.Siti Azizah
09/19/2013
16S rRNA; COI; Mitochondrial DNA gene sequences; Molecular phylogeny; Octopodidae species

Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c ... more

Z.M. Lü; W.T. Cui; L.Q. Liu; H.M. Li; C.W. Wu
06/21/2013
Biological control; COI; Genetic structure; Intraspecific divergence; Morphological variation; Placusa pinearum

Placusa pinearum, a newly described beetle species found living in pine shoot tunnels bored by the pine tip moth, Dioryctria rubella (Pyralidae), has potential as a vector to transport biological agents for controlling this moth pest of pine trees because of the high degree of niche overlap (co-occurrence) between them. In order to help determine the suitability of ... more

J.Y. Gao; B.Z. Ji; S.W. Liu
05/09/2014
16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
04/29/2014
Chinese black-bone chicken; Cyt b; Genetic diversity; Maternal origin

Chinese black-bone chickens are valued for the medicinal properties of their meat in traditional Chinese medicine. We investigated the genetic diversity and systematic evolution of Chinese black-bone chicken breeds. We sequenced the DNA of 520 bp of the mitochondrial cyt b gene of nine Chinese black-bone chicken breeds, including Silky chicken, Jinhu black-bone chicken, Jiangshan black- ... more

W.Q. Zhu; H.F. Li; J.Y. Wang; J.T. Shu; C.H. Zhu; W.T. Song; C. Song; G.G. Ji; H.X. Liu
12/14/2006
Crinipellis perniciosa; Filamentous fungi; Genomic DNA extraction; PCR

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was ... more

S.C.O. Melo; C. Pungartnik; J.C.M. Cascardo; M. Brendel
10/26/2006
Ganga; Hooghly; Narmada; PCR; RAPD; Tenualosa ilisha; Yamuna

RAPD was used to delineate the hilsa populations sampled from the Ganga, Yamuna, Hooghly, and Narmada Rivers at six different locations. Six degenerate primers were used to generate the fragment patterns from the samples collected. All primers were highly polymorphic and generated high numbers of amplification products. Nei’s genetic distances were calculated between locations. The ... more

M.P. Brahmane; M.K. Das; M.R. Sinha; V.V. Sugunan; A. Mukherjee; S.N. Singh; S. Prakash; P. Maurye; A. Hajra
12/08/2011
Amplification success; Barcoding; Birds; Cytochrome oxidase 1; Mammals; Mini-barcode; PCR; Reptiles

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single ... more

I.A. Arif; H.A. Khan; A. Sadoon; M. Shobrak
10/27/2011
BLV testing; Bovine leukemia virus; Cattle; Leukosis; Native Iranian and Russian breeds; PCR

Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

M.R. Mohammadabadi; M. Soflaei; H. Mostafavi; M. Honarmand
08/05/2011
Cereal seeds; DNA extraction; PCR

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves ... more

Z.H. Pervaiz; N.A. Turi; I. Khaliq; M.A. Rabbani; S.A. Malik

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