Research Article

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Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 ... more

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ISSR; Laboratory optimization; Method evaluation; Molecular marker; PCR

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E.C. Oliveira; A.T.Amaral Júnior; L.S.A. Gonçalves; G.F. Pena; S.P.Freitas Júnior; R.M. Ribeiro; M.G. Pereira
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Genetic variability; ISSR; Mandacaru; morphology; RAPD

This is the first study to examine the genetic diversity of mandacaru cactus (Cereus jamacaru P. DC.). Plants of spineless mandacaru are commonly found in gardens and parks of urban areas in northeastern Brazil. In addition to exploring their ornamental potential, morphological, and genetic characterization may contribute to the development of plant materials that can be used as ... more

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Fusarium verticillioides is a pathogen of agriculturally im­portant crops, especially maize. It is considered one of the most impor­tant pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and ani­mals. Moreover, it is recognized as a cause of localized infections in im­munocompetent patients and ... more

S.C. Chang; D.P.C. Macêdo; C.M. Souza-Motta; N.T. Oliveira
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Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed ... more

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IRAP; ISSR; Mating-type; Musa sp; Polymorphism

The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 ... more

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Agronomic traits; Genetic variation; Gossypium hirsutum L.; ISSR; RAPD; SSR

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers ... more

Z. Noormohammadi; H.A. Farahani; M. Sheidai; S. Ghasemzadeh-Baraki; O. Alishah
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Achillea species; Genetic diversity; ISSR; Marker; RAPD

Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, ... more

M. Ebrahimi; M. Farajpour; M. Rahimmalek
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ISSR; RAPD; Sequence characterized amplification region; Thinopyrum elongatum

Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We used 36 RAPD primers and 33 ISSR primers to ... more

G.H. Xu; W.Y. Su; Y.J. Shu; W.W. Cong; L. Wu; C.H. Guo

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