Related GMR Articles

Fluorescence in situ hybridization; Freshwater fishes; Karyotype evolution; Molecular cytogenetics; Ribosomal DNA

In the present study, the karyotype and chromosomal characteristics of 9 species of the Bagridae fish family were investigated using conventional Giemsa staining as well as dual-color fluorescence in situ hybridization to detect the 18S and 5S rDNA sites. In addition to describing the karyotype of several Bagridae catfishes, we established molecular cytogenetic techniques to ... more

W. Supiwong; T. Liehr; M.B. Cioffi; A. Chaveerach; N. Kosyakova; X. Fan; T. Tanee; A. Tanomtong
Comparative genomic hybridization; Embryos; Fluorescence in situ hybridization

We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined individual blastomeres from arrested embryos by FISH ... more

M.C. Muhlmann; A.O. Laudicina; C. Perandones; M.V. Bertolino; A. Marazzi; C.J. Quintans; M. Donaldson; W. Bozzo; S. Pasqualini
Alien substitution line; Leaf rust; Leymus mollis; Molecular cytogenetics; Triticum aestivum

Leymus mollis (Trin.) Pilger (NsNsXmXm, 2n = 28), a wild relative of common wheat, possesses many traits that are potentially valuable for wheat improvement. In order to exploit and utilize the useful genes of L. mollis, we developed a multiple alien substitution line, 10DM50, from the progenies of octoploid Tritileymus M842-16 x Triticum durum cv. ... more

Y.H. Pang; J.X. Zhao; W.L. Du; Y.L. Li; J. Wang; L.M. Wang; J. Wu; X.N. Cheng; Q.H. Yang; X.H. Chen
Homologous Gene Quantitative PCR (HGQ-PCR); Molecular cytogenetics; Quantitative Fluorescent PCR (QF-PCR); SNP-arrays; Spontaneous abortions

Spontaneous abortions, which occur in 15-20% of all pregnancies, constitute the most common human genetic disease, since most miscarriages are caused by chromosomal anomalies. In our laboratory, we devised a protocol for sequential use of quantitative fluorescent PCR (QF-PCR), homologous gene quantitative PCR (HGQ-PCR) and SNP-arrays that allows examination of ... more

C.S. de Sousa; H.B. Pena; J.L.F. Rocha; S.D.J. Pena
Fluorescence in situ hybridization; Molossid; Phyllostomid; Chiroptera; Telomeric sequence

Molossidae species, Cynomops abrasus (2n = 34, fun­damental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyl­lostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with ... more

K.C. Faria; S.R.C. Marchesin; P.R.L. Moreira; M.R. Beguelini; E. Morielle-Versute
Acute promyelocytic leukemia; Cryptic insertions; Fluorescence in situ hybridization; promyelocytic leukemia/retinoic acid receptor alpha; Reverse transcription-polymerase chain reaction; Sequencing analysis

Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients; most negative results are due to technical problems or ... more

A. Choughule; S. Polampalli; P. Amre; S. Shinde; S. Banavali; K. Prabhash; R. Nair; P.G. Subramanian; S. Gujral; P.M. Parikh
deletion; Essential hypertension; Fluorescence in situ hybridization; subtelomere; telomere

Telomere biology is intimately linked to the genetic/environmental etiology of cardiovascular and metabolic diseases and telomere shortening is emerging as an important biomarker disease. The relationship between subtelomeric deletions and genetic hypertension was examined. Fluorescence in situ hybridization was used to directly assess whether there is a loss or gain of subtelomere ... more

S.T. Onrat; A.G. Tomatir
Autism spectrum disorders; Chromosome 2; FISH technique; Fluorescence in situ hybridization

Autism spectrum disorders are severe psychiatric diseases commonly identified in the population. They are diagnosed during childhood and the etiology has been much debated due to their variations and complexity. Onset is early and characterized as communication and social interaction disorders and as repetitive and stereotyped behavior. Austistic disorders may occur together with various ... more

A. Barbosa-Gonçalves; C.B. Vendrame-Goloni; A.L.B. Martins; A.C. Fett-Conte
BCR-ABL fusion; Chronic myelogenous leukemia; Cytogenetic remission; Disease-free survival; Fluorescence in situ hybridization; Stem cell transplantation

Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ ... more

C.B. Vendrame-Goloni; A.B. Carvalho-Salles; M.A. Ruiz; R. Júnior; M. Varella-Garcia; A.C. Fett-Conte
Aneuploidy screening; Fluorescence in situ hybridization; PGAS

Preimplantation genetic diagnosis is a preventive approach for identifying genetic abnormalities in early stages of reproduction. We used preimplantation genetic aneuploidy screening in 230 cycles of patients with indications of advanced maternal age, recurrent implantation failure, recurrent spontaneous abortions, or severe male factor. Biopsied blastomeres from embryos with six to eight ... more

N. Ercelen; E. Turtar; M. Gultomruk; H. Comert; H. Coskun; R. Mercan; A. Nuhoglu