Research Article

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12/13/2013
Mastitis; PCR-PIRA; PCR-RFLP; Percentage of fat; PGLYRP-2 gene; Polymorphism

Peptidoglycan recognition protein 2 (PGLYRP-2), which belongs to the PGRP family, is the only member that has no direct bactericidal activity but has N-acetylmuramoyl-l-alanine amidase activity. This feature of PGLYRP-2 indicates that it may play an important role in eliminating the pathogen associated molecular pattern (PAMP), such as peptidoglycan (PGN), which can reduce ... more

H.L. Wang; Z.X. Li; L. Chen; J. Yang; L.J. Wang; H. He; F.B. Niu; Y. Liu; J.Z. Guo; X.L. Liu
12/13/2013
Beef cattle; Carcass and meat quality traits; Myf6; MyoD1; PCR-RFLP; Polymorphism

Myogenic determination factor 1 (MyoD1) and myogenic factor 6 (Myf6) genes belong to the myogenic differentiation (MyoD) gene family, which play key roles in growth and muscle development. The study aimed to investigate the effects of variants in cattle MyoD1 and Myf6 on carcass and meat traits. We screened single nucleotide polymorphisms (SNPs ... more

X.H. Du; Q.F. Gan; Z.R. Yuan; X. Gao; L.P. Zhang; H.J. Gao; J.Y. Li; S.Z. Xu
04/03/2014
Bovine papillomavirus; New putative Deltapapillomavirus; PCR-RFLP; Phylogenetics; Sequencing

Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for ... more

T.C. Melo; R.F. Carvalho; J. Mazzucchelli-de-Souza; N. Diniz; S. Vasconcelos; S.L.M.R. Assaf; R.P. Araldi; R.M. Ruiz; I. Kerkis; W. Beçak; R.C. Stocco
02/13/2014
GFI1B gene; Growth trait; Indigenous Chinese goats; PCR-RFLP; PCR-SSCP

This study aimed to investigate polymorphisms of the eighth exon in the GFI1B gene among three indigenous Chinese goat breeds (QianBei Ma goats, GuiZhou white goats, and GuiZhou black goats). Furthermore, association analysis was conducted between these polymorphisms and growth traits. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), direct DNA ... more

H.F. Cai; Z. Chen; W.X. Luo
04/27/2015
cpDNA; mtDNA; PCR-RFLP; Plum; Prunus insititia; Prunus salicina

Chloroplast (cpDNA) and mitochondrial DNA (mtDNA) were analyzed to establish genetic relationships among Tunisian plum cultivars using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Two mtDNA regions (nad 1 b/c and nad 4 1/2) and a cpDNA region (trnL-trnF) were amplified and digested using restriction ... more

S. Ben Mustapha; H. Ben Tamarzizt; G. Baraket; D. Abdallah; S. Hannachi
09/18/2002
Brazil; Glutathione S-transferase; Polymorphism

The glutathione S-transferase (GST) family of enzymes has a vital role in phase II of biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic, with the type and frequency of polymorphism being ethnic dependent. Polymorphisms in GST genes have been shown to be associated with susceptibility to disease and disease outcome. We ... more

A. Rossini; D.C.M. Rapozo; L.M.F. Amorim; J.M.B. Macedo; R. Medina; J.F.N. Neto; C.V.M. Gallo; L.F.R. Pinto
03/28/2007
Dog breeds; Growth; Polymorphism; SNP; SOCS2

Suppressor of cytokine signaling 2 (SOCS2) is a negative regulator of growth hormone signaling. The deletion of SOCS2 in mice results in a 30-50% increase in post-natal growth. In an effort to identify polymorphisms in the SOCS2 gene that may be associated with body size in dogs, we characterized the canine SOCS2 gene and analyzed its genetic diversity among small and large dog breeds ... more

G. Rincón; A.E. Young; D.L. Bannasch; J.F. Medrano
03/14/2006
Alu; CEPH panel; Human population genetics; Polymorphism; X chromosome

We describe a novel polymorphic Alu insertion (DXS225) on the human X chromosome (Xq21.3) embedded into an L1 retrotransposon. The DXS225 polymorphism was genotyped in 684 males from the CEPH Human Genome Diversity Panel. This insertion was found in all regions of the globe, suggesting that it took place before modern humans spread from Africa ca. 100,000 ... more

R.Wellerson Pereira; S.Silva dos Santos; S.Danilo Jun Pena
01/05/2009
meningiomas; metastases; Methylation; Polymorphism; schwannomas; TP53

The p53 tumor suppressor gene is the most frequently mutated gene in human cancer; this gene is mutated in up to 50% of human tumors. It has a critical role in the cell cycle, apoptosis and cell senescence, and it participates in many crucial physiological and pathological processes. Polymorphisms of p53 have been suggested to be associated with genetically determined susceptibility in various ... more

L.O. Almeida; A.C. Custódio; G.R. Pinto; M.J. Santos; J.R.W. Almeida; C.A. Clara; J.A. Rey; C. Casartelli
02/17/2009
Bombyx mori multiple nucleopolyhedrovirus; Molecular markers; Polymorphism; Random amplified polymorphic DNA; Silkworm

The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance to baculovirus infection to analyze the ... more

L.F.C. Ribeiro; D.B. Zanatta; J.P. Bravo; R.M.C. Brancalhão; M.A. Fernandez

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