Research Article

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12/05/2006
CG content; Hyperthermophiles; Microsatellites; Optimum growth temperature; SSRs; Thermophiles

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of ... more

S. Trivedi
07/13/2006
Ancestry; Microsatellites; Synthetic breed; Taurine; Zebu

The International Society of Animal Genetics (ISAG) has chosen nine microsatellites (international marker set) as a standard that should be included in all cattle parentage studies. They are BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, and ETH225. We decided to ascertain whether this microsatellite set could be used to determine ancestral proportions in ... more

H.M.S. Bicalho; C.G. Pimenta; I.K.P. Mendes; H.B. Pena; E.M. Queiroz; S.D.J. Pena
03/18/2008
Genetic variability; Horse; Microsatellites; Pantaneiro horses

The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity ... more

E.H. Giacomoni; G.P. Fernández-Stolz; T.R.O. Freitas
10/07/2011
Horse; Microsatellites; South America; Venezuelan Criollo

Various horse populations in the Americas have an origin in Spain; they are remnants of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). We evaluated genetic variability within the Venezuelan Criollo horse and its relationship with other horse breeds. We observed high levels of genetic diversity within the Criollo breed. Significant ... more

E.G. Cothran; J.L. Canelon; C. Luis; E. Conant; R. Juras
06/21/2011
Genetic diversity; Microsatellites; SSR markers; Wild sweet cherry

Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a ... more

S. Ercisli; G. Agar; N. Yildirim; H. Karlidag; B. Duralija; A. Vokurka
05/31/2011
Conservation; Genetic diversity; Microsatellites; Pelodiscus sinensis

Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and detected high levels of polymorphism for all 21 loci, ... more

X.J. Bu; L. Liu; L. Wang; L.W. Nie
04/26/2011
CART gene; Goats; Microsatellites; Reproduction traits; SNPs

Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, ... more

P.Q. Wang; L.M. Deng; B.Y. Zhang; M.X. Chu; J.Z. Hou
04/26/2011
Conservation; Genetic variation; Microsatellites; RAMs; Scleropages formosus

Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The number of alleles at the 10 microsatellite loci ranged ... more

B. Manoharan; Z. Sulaimen; F. Omar; R.Y. Othman; S.Z. Mohamed; S. Bhassu
01/05/2010
Comparative analysis of molecular groups; DNA markers; Microsatellites; RAPD and SSR loci; Zea mays

Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using both RAPD and SSR markers, and we evaluated how well these two ... more

A.A. Leal; C.A. Mangolin; A.Tdo Amaral Júnior; L.S.A. Gonçalves; C.A. Scapim; A.S. Mott; I.B.O. Eloi; V. Cordovés; M.F.P. da Silva
08/12/2013
Alectrurus risora; Culicivora caudacuta; Microsatellites; Tyrannidae

Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culicivora caudacuta), ... more

B. Mahler; A.R.R. Schneider; A.S. Di Giacomo; A.G. Di Giacomo; J.C. Reboreda; R. Tiedemann

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