Research Article

Related GMR Articles

12/09/2008
Caryocar brasiliense; Doxorubicin; Drosophila melanogaster; Genotoxicity; Pequi; SMART

Caryocar brasiliense Camb. is a tree popularly known in Central Brazil as pequi. Its fruit contains carotenes, retinols, vitamin C, and polyphenols. These compounds possess antioxidant properties preventing excessive free radical formation and modulating the genotoxicity of physical and chemical agents in the body. However, at high concentrations these compounds can have ... more

A.J.S. Castro; C.K. Grisolia; B.C. de Araújo; C.D. Dias; E.S. Dutra; J.C. Nepomuceno
11/17/2009
Antigenotoxicity; Antioxidants; Doxorubicin; Drosophila melanogaster; SMART; β-carotene

β-carotene (BC), pro-vitamin A, is an efficient antioxidant, effective in the neutralization of oxygen reactive species, which cause serious damage to DNA. Various studies have been conducted on the effectiveness of BC for chemoprevention of cancer and heart disease. Doxorubicin is a chemotherapeutic agent used for cancer treatment that generates free radicals. We examined the effects of ... more

C.D. Dias; B.C. Araújo; E.S. Dutra; J.C. Nepomuceno
05/25/2015
Autophagy; Doxorubicin; Drug resistance; Myeloma; RPMI8226

We investigated the effect of autophagy on drug resistance of multiple myeloma (MM) to doxorubicin (DOX). A DOX-resistant MM cell line (RPMI8226/DOX) was developed by progressively increasing the DOX concentration gradient. The drug resistance index was determined using the MTT method. Transmission electron microscopy, anti-light chain 3-fluorescein isothiocyanate immunofluorescence, and ... more

Y.Z. Pan; X. Wang; H. Bai; C.B. Wang; Q. Zhang; R. Xi
03/10/2003
Apoptosis; Drosophila melanogaster; Eye development; UAS/GAL4 ectopic expression system

The UAS/GAL4 ectopic expression system is widely used in Drosophila melanogaster for the overexpression of transgenes. This system operates under the assumption that the yeast transcription factor, GAL4, is inactive in D. melanogaster. Thus, GAL4 can be expressed under the control of D. melanogaster-specific promoters with little effect upon the organism. We ... more

J.M. Kramer; B.E. Staveley
07/28/2009
Drosophila melanogaster; Drosophila simulans; Interspecific crosses; Wolbachia

Wolbachia are bacteria that live inside the cells of a large number of invertebrate hosts and are transmitted from infected females to their offspring. Their presence is associated with cytoplasmic incompatibility in several species of Drosophila. Cytoplasmic incompatibility results when the sperm of infected males fertilize eggs of uninfected females, causing more or ... more

I.N. Gazla; M.C. Carracedo
03/22/2011
Climbing; Drosophila melanogaster; Green fluorescent protein (GFP); Longevity

Green fluorescent protein (GFP) is widely used as a reporter transgene in a variety of organisms. Some of the advantages of using GFP include non-invasive visualization of biological events and/or tissues in live specimens and its benign nature. When GFP is expressed throughout the organism, in neurons and eyes, lifespan and climbing ability of flies are ... more

R.M.S. Mawhinney; B.E. Staveley
01/05/2011
Anti-genotoxicity; Drosophila melanogaster; Genotoxicity; Luehea divaricata; SMART/wing

Luehea divaricata is a native plant of the Brazilian Cerrado, known as “açoita-cavalo”. It is used as a popular herbal medicine in the treatment of dysentery, bleeding, arthritis, tumors, ulcers, and gangrenous wounds. Considering that herbal medicines sometimes provoke tumors and/or may prevent mutational events, it is important to study the action of these natural drugs on DNA ... more

L.P. Felício; E.M. Silva; V. Ribeiro; C.T. Miranda; I.L.B.F. Vieira; D.C.S. Passos; A.K.S. Ferreira; C.R. Vale; D.C.S. Lima; S. Carvalho; W.B. Nunes
10/13/2010
Drosophila melanogaster; Drosophila sechellia; Drosophila simulans; QTL mapping; Quantitative variation; Transgressive segregation

The Drosophila wing has been used as a model in studies of morphogenesis and evolution; the use of such models can contribute to our understanding of mechanisms that promote morphological divergence among populations and species. We mapped quantitative trait loci (QTL) affecting wing size and shape traits using highly inbred introgression lines between D. simulans and ... more

B.P. Matta; B.C. Bitner-Mathé
01/19/2010
Anti-genotoxicity; Byrsonima verbascifolia; Drosophila melanogaster; Murici; SMART/wing

Byrsonima verbascifolia, popularly known in Brazil as murici, is a medicinal plant widely used in the treatment of bacterial and viral infections, Chagas´s disease, diarrhea, bronchitis, cough and fever, as well as for protection of the intestinal mucosa. Since chemotherapy and radiotherapy, broadly employed in the treatment of cancer, can have undesirable side effects, such as ... more

D.M. Mendanha; H.D. Ferreira; L.P. Felício; E.M. Silva; D.G. Pereira; W.B. Nunes; S. Carvalho
03/22/2012
Drosophila melanogaster; Genome plasticity; hsp22 downregulation; RT-PCR

A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The ... more

L.M. Magdalena; E.C. Coipan; A.F. Vladimirescu; L. Savu; M. Costache; L. Gavrila

Pages