Research Article

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The black oat (Avena strigosa Schreb) is commonly used for forage, soil cover, and green manure. Despite its importance, little improvement has been made to this species, leading to high levels of genotypic disuniformity within commercial cultivars. The objective of this study was to evaluate the efficiency of different doses of gamma rays [60Co] applied to black oat ... more

G. Silveira; E. Moliterno; G. Ribeiro; P.M.A. Costa; L.G. Woyann; E.W. Tessmann; A.C. Oliveira; C.D. Cruz
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Comet assay; Micronucleus test; Occupational exposure

The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were ... more

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Chromosome aberration; Comet assay; Rotenone

Rotenone is a heterocyclic compound widely used as an insecticide, acaricide and piscicide. Its toxicity is mainly caused by the inhibition of mitochondrial respiratory processes and ATP production, resulting in the generation of reactive oxygen species. Reactive oxygen species can interact with DNA, RNA and proteins, leading to cell damage, followed by death. We used the Comet assay, and we ... more

P.Danielle L. de Lima; E.Sumi Yamada; E.Tavares da Costa; Cdo O. Pessoa; S.Helena Bar Rabenhorst; Mde Oliveir Bahia; P.Cerqueira Cardoso; R.Alves Santos; Mde Arruda Smith; Mde Arruda Smith; R.Rodríguez Burbano
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Antioxidant enzyme polymorphisms; Biochemical markers; Caryocar brasiliense; Comet assay; Exercise-induced damage; TBARS assay

Physical training induces beneficial adaptations; however, exhausting exercise increases reactive oxygen species generation, resulting in damage to DNA and tissues. Pequi (Caryocar brasiliense), a fruit of the Brazilian Cerrado, contains a carotenoid-rich oil. We investigated whether pequi oil had antioxidant effects in runners. Evaluations were made after outdoor races before ... more

A.L. Miranda-Vilela; A.K. Akimoto; P.C.Z. Alves; L.C.S. Pereira; C.A. Gonçalves; M.N. Klautau-Guimarães; C.K. Grisolia
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Comet assay; Medicinal plant; Micronucleus test; Single-cell gel electrophoresis; Tamarindus indica

Tamarindus indica has been used in folk medicine as an antidiabetic, a digestive aid, and a carminative, among other uses. Currently, there is no information in the toxicology literature concerning the safety of T. indica extract. We evaluated the clastogenic and/or genotoxic potential of fruit pulp extract of this plant in vivo in peripheral blood and liver ... more

F.M.V. Silva; M.F. Leite; A.C.C. Spadaro; S.A. Uyemura; E.L. Maistro
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Allium cepa; Chromosomal aberrations; Comet assay; Nuclear abnormalities; Oreochromis niloticus; Water pollution
L.R. Maschio
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Antioxidant; Comet assay; haptoglobin polymorphism; hydrogen peroxide

Human haptoglobin is classified into three major phenotypes: Hp1-1, Hp2-1 and Hp2-2; there are two autosomal alleles Hp*1 and Hp*2, and the Hp*1 allele has two subtypes, Hp*1F and Hp*1S. Haptoglobin acts as an antioxidant, preventing hemoglobin-driven oxidative damage. We used the comet assay to ... more

L.R.S. Moreira; A.L. Miranda-Vilela; I.C.R. Silva; A.K. Akimoto; M.N. Klautau-Guimarães; C.K. Grisolia
04/22/2009
Ambelania occidentalis; Comet assay; Micronucleus test; Mutagenicity assay; Single-cell gel electrophoresis

Ambelania occidentalis is routinely used in folk medicine for treating gastrointestinal disorders, even though there have been no safety trials. We evaluated the genotoxic potential of hydro-alcoholic extracts of this plant in mice; induced DNA damage was assessed in peripheral blood leukocytes and micronucleus induction was assessed in polychromatic erythrocytes from bone marrow. The ... more

L.S. Castro; F.F. Perazzo; E.L. Maistro
12/02/2008
Chromosome aberrations; Clusia alata; Comet assay; Single-cell gel electrophoresis

We examined the genotoxic and mutagenic effects of a crude extract of Clusia alata (a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was injected intraperitoneally. ... more

A.C.G. Moura; F.F. Perazzo; E.L. Maistro
08/14/2008
Comet assay; Micronucleus test; Musa paradisiaca; peripheral blood cells; Single-cell gel electrophoresis

Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000 ... more

C.U.B. Andrade; F.F. Perazzo; E.L. Maistro

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