Research Article

Related GMR Articles

09/09/2008
Amazona aestiva; bottleneck; Conservation; Genetic structure; Microsatellite

The blue-fronted Amazon (Amazona aestiva) is a widely distributed Neotropical parrot and one of the most captured parrots in nature to supply the illegal trade of wild animals. The objectives of the present study were to analyze the genetic structure of A. aestiva to identify management units and support conservation planning and to verified if A. aestiva populations ... more

K.C.E. Leite; G.H.F. Seixas; I. Berkunsky; R.G. Collevatti; R. Caparroz
11/22/2011
bees; Conservation; Melipona; Microsatellite; Transferability

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Melipona has the ... more

M.V.C. Viana; E.A. Miranda; A.K. de Francisco; C.A.L. Carvalho; A.M. Waldschmidt
07/25/2011
Coilia mystus; Conservation; Microsatellite; PIMA; RAPD-PCR enrichment

Coilia mystus is the most important harvested fish species in China; it inhabits quite different water environments during the different life history stages. Populations of C. mystus have dropped sharply due to overharvesting and water pollution. We developed eight microsatellite loci in C. mystus for conservation genetics studies. These new markers were tested ... more

J.Q. Yang; X.D. Zhou; D. Liu; Z.Z. Liu; W.Q. Tang
05/23/2014
Cervus unicolor; Conservation; Microsatellite; PCR-based isolation of microsatellite arrays; RAPD-PCR enrichment

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg ... more

D.Y. Lin; T.Y. Chiang; C.C. Huang; H.D. Lin; S.J. Tzeng; S.R. Kang; H.M. Sung; M.C. Wu
04/14/2014
Conservation; Greek black pig; Microsatellite

Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays, the worldwide distribution of pigs is dominated by a few breeds, tending towards a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. The Greek black pig, a breed raised locally and known for the high quality of its meat for cured ... more

S. Michailidou; A. Kalivas; I. Ganopoulos; E. Stea; G. Michailidis; A. Tsaftaris; A. Argiriou
01/10/2014
Common dormouse; Conservation; Genetic structure; Microsatellite; Movement

Movement is an important life history trait that can have an impact on local adaptation, and other evolutionary phenomena. We used a combination of nestbox survey data and genetic techniques (genotyping at 10 microsatellite loci) to quantify patterns of movement in common dormice Muscardinus avellanarius at two distinct sites in the UK: 1) Bontuchel (a natural population) and 2) ... more

D.M. Naim; S. Telfer; S. Tatman; S. Bird; S.J. Kemp; P.C. Watts
06/12/2015
Conservation; Holothuria scabra; Microsatellite; Sea cucumber

The sea cucumber Holothuria scabra is an endangered species. In this study, nine new polymorphic microsatellite loci were developed and tested in 30 individuals. The number of alleles ranged from 2 to 5, and the observed and expected heterozygosities ranged from 0.1200 to 0.7391 and from 0.2408 to 0.5983, respectively. No loci significantly deviated from the Hardy-Weinberg ... more

Z.B. Li; G. Dai; J.B. Shangguan; Y.F. Ning; Y.Y. Li; R.B. Chen; Y.S. Huang; Y. Yuan
03/15/2007
Acridine orange test; Bovine; Conservation; DNA; Freeze-drying sperm; TUNEL

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different ... more

C.F. Martins; M.N. Dode; S.N. Báo; R. Rumpf
12/01/2006
Amazon; Brachyplatystoma; Conservation; Fish homing; Phylogeography; Siluriformes

The large pimelodid, Brachyplatystoma rousseauxii, is one of the two most important catfish species for the fisheries in the Amazon. It is captured by commercial and artisanal fishing fleets in at least five Amazonian countries, at fishing grounds more than 5000 km apart. Current evidence suggests a complex life cycle that includes the longest reproductive migration known for a ... more

J.S. Batista; J.A. Alves-Gomes
02/25/2009
Conservation; Geometric morphometry; Meliponini; Sex differentiation; species identification; Wing morphometry

Currently, the identification of pollinators is a critical necessity of conservation programs. After it was found that features extracted from patterns of wing venation are sufficient to discriminate among insect species, various studies have focused on this structure. We examined wing venation patterns of males and workers of five stingless bee species in order to determine if there are ... more

T.M. Francoy; R.A.O. Silva; P. Nunes-Silva; C. Menezes; V.L. Imperatriz-Fonseca

Pages