Research Article

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Apomixis; Molecular marker; Satellite technique

Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using five ... more

N.M.A. Nassar; R.G. Collevatti
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DNA sequence; Gene-rich region; Molecular marker; Resistance; Yellow rust

Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D ... more

E. Cabuk; Y. Aydin; A.A. Uncuoglu
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Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
11/29/2011
Genetic diversity; Molecular marker; Piper; RAPD; SRAP

Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analysis were applied to 74 individual plants of Piper spp in Hainan Island. The results showed that the SRAP technique may be more informative and more efficient and effective for studying genetic diversity of Piper spp than the RAPD technique. The overall level of genetic ... more

Y. Jiang; J.P. Liu
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ISSR; Molecular marker; RAPD

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 ... more

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Harpadon nehereus; Microsatellite; Molecular marker

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)15-enriched ... more

T.J. Xu; D.Q. Sun; H.Y. Li; R.X. Wang
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Adulteration; AFLP; DNA fingerprinting; Molecular marker; Zingiber spp

The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic ... more

S. Ghosh; P.B. Majumder; S. Mandi
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Microsatellite; Molecular marker; Scartelaos viridis

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences contained multiple repeat motifs ( ... more

D.Q. Sun; H.Y. Li; T.J. Xu; R.X. Wang
12/21/2010
Biodiversity conservation; Coefficient of similarity; genetic breeding; Genetic variability; Grouping analyses; Molecular marker

The genetic diversity and characteristics of commercial interest of Passiflora species make it useful to characterize wild germplasm, because of their potential use for fruit, ornamental and medicinal purposes. We evaluated genetic diversity, using RAPD markers, of 32 genotypes of Passiflora cincinnata collected from the wild in the region of Vitória da Conquista, Bahia ... more

C.B.M. Cerqueira-Silva; L.D.H.C.S. Conceição; E.S.L. Santos; C.B. Cardoso-Silva; A.S. Pereira; A.C. Oliveira; R.X. Corrêa
01/11/2011
C. equisetifolia subsp incana; Casuarina equisetifolia subsp equisetifolia; Genetic diversity; Molecular marker; Northern coast of Senegal; RAPD

The genetic variation of 70 individual samples of Ca­suarina equisetifolia (L. Johnson) subsp equisetifolia and C. equiseti­folia subsp incana growing along the northern coast of Senegal was analyzed with RAPD markers. Of the 160 primers tested, five were chosen; they generated 1396 reproducible bands and 61 polymorphic bands that were scored. This ... more

A.L. Ndoye; O. Sadio; D. Diouf

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