Research Article

Related GMR Articles

Aedes aegypti; Isodillapiole; qRT-PCR

The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have ... more

V.S. Lima; A.C. Pinto; M.S. Rafael
cDNA clone; Indigenous Chinese goats; PCR-SSCP; qRT-PCR; RERG gene

The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations with growth traits in three varieties of ... more

M.X. Sui; H.H. Wang; Z.W. Wang
Coffea arabica; Developmental stages; qRT-PCR

Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze ... more

F.C. Santos; A.C.S. Clemente; F. Caixeta; S.D.V.F. Rosa
AHL25; Arabidopsis thaliana; Artificial micro-RNA; AtExpA8; qRT-PCR

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis ... more

A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah
Immune response; Malaria; Phylogenetic relationship; qRT-PCR; Resistance to insecticides
Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection
of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been
used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated
to feeding and resistance to insecticides, immune response and ... more
Ohse; K. C; Matiolli; C. C; Scarpassa; V. M; Santos; V. S; Tadei; W. P; Rafael; M. S
Abscisic acid; Coffea arabica; Gibberellic acid; qRT-PCR

During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of ... more

F.Cdos Santos; S.D.V.F. Rosa; S.V.B. Coelho; A.C.S. Clemente; T.B. Fantazzini; C.C. Pereira; F. Caixeta; N.A.B. Fávaris; J.L. Baúte
Callus; Gene cloning; Isoflavone synthase; Sequence analysis; Trifolium pretense

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and ... more

H.H. Hu; C.Q. Jing; R. Liu; W.D. Li; H.G. Feng
Bambusa oldhamii; Gene cloning; Sequence analysis; TERMINAL FLOWER1; Transgenic Arabidopsis thaliana

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1- ... more

H.Y. Zeng; Y.T. Lu; X.M. Yang; Y.H. Xu; X.C. Lin
Gene cloning; Hami melon; Real-time fluorescence quantitative PCR; Superoxide dismutase

Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the cloned gene fragment was ... more

C.H. Shan; F.X. Tang; W. Chen; W.R. Ma
Eruca vesicaria subsp sativa; Gene cloning; Nicotianamine synthase

Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. In this study, a ... more

B.L. Huang; C. Cheng; G.Y. Zhang; J.J. Su; Y. Zhi; S.S. Xu; D.T. Cai; X.K. Zhang; B.Q. Huang