Research Article

Related GMR Articles

07/14/2015
Acipenser schrenckii; Genetic diversity; Microsatellite; Next-generation sequencing; Polymorphism

Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more infor­mation on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the develop­ment of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of ... more

L.M. Li; L. Wei; H.Y. Jiang; Y. Zhang; X.J. Zhang; L.H. Yuan; J.P. Chen
07/13/2015
Microsatellite; Population genetics; Purpleback flying squid; Sthenoteuthis oualaniensis

The purpleback flying squid (Sthenoteuthis oualaniensis) is a pelagic squid with tremendous potential for commercial exploitation. We isolated and characterized 21 polymorphic microsatellite loci for S. oualaniensis using a (GT)13-enriched genomic library. The number of alleles per locus varied from 6 to 32. The observed and expected heterozygosities ranged ... more

L. Lin; P. Li; Z.Z. Chen; Y.Y. Xiao; S.N. Xu; Y. Liu; C.H. Li
12/09/2015
Conservation; Microsatellite; Population genetics; Pteroceltis tatarinowii

Pteroceltis tatarinowii (Ulmaceae) is a scientifically and economically important temperate deciduous tree that is endemic to China. In the present study, 12 P. tatarinowii polymorphic microsatellite loci were developed using the tailed primer-M13-simple sequence repeats (TP-M13-SSR) biotin-capture method. The number of alleles per locus ranged from 2 to 10, with an ... more

X.H. Li; X.P. Zhang; K. Liu; H.J. Liu; J.W. Shao
04/04/2016
Aquatic herb; Brasenia schreberi; Endangered species; Microsatellite marker; Next-generation sequencing

To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the ... more

H. Liu; Z.C. Long; L.N. Li; Q.F. Wang; J.M. Chen; K. Liao; H. Liu; Z.C. Long; L.N. Li; Q.F. Wang; J.M. Chen; K. Liao; H. Liu; Z.C. Long; L.N. Li; Q.F. Wang; J.M. Chen; K. Liao
11/03/2016
Keratoconus; Mutation; Next-generation sequencing; VSX1

The aim of this study was to screen the visual system homeobox 1 (VSX1) gene in Turkish patients with keratoconus (KC). The patient group consisted of 44 patients who had undergone corneal transplant surgery before the age of 30, for advanced and rapidly progressive KC. The control group comprised 250 healthy individuals. We ... more

H. Bardak; M. Gunay; E. Yildiz; Y. Bardak; B. Gunay; H. Ozbas; O. Bagci
11/21/2016
Loci transferability; Microsatellite loci; Next-generation sequencing; Sillago japonica; Sillago sihama

Sillago sihama (Forsskål, 1775), a commercially important marine fishery species in the Indo-West Pacific, is being developed as a target species for aquaculture and stock enhancement in China. However, due to the limitations of traditional isolation methods, the available microsatellite loci, or simple sequence repeats (SSRs), of S. sihama lack ... more

R.X. Wu; H.R. Zhang; S.F. Niu; Y. Zhai; X.F. Liu; R.X. Wu; H.R. Zhang; S.F. Niu; Y. Zhai; X.F. Liu
10/05/2018
DNA; miRNA; mRNA; Next-generation sequencing; Phalaenopsis; PhalDB

The orchid family Orchidaceae is one of the largest families of angiosperms; it includes approximately 35,000 species, displays a wide range of unique flower shapes and is a valued ornamental crop. Among the various species of orchids, the Phalaenopsis genus is the most widely commercialized among blooming potted plants. Our aim was to provide in-depth ... more

C.Y. Lee; K.K. Viswanath; J.Z. Huang; C.P. Lee; C.P. Lin; T.C. Cheng; B.C. Chang; S.W. Chin; F.C. Chen
08/28/2015
Cold tolerance; Low temperature; Microsatellite; Oreochromis niloticus

Tilapia is an important fish cultured in tropical and subtropical areas. Cold sensitivity limits the expansion of tilapia culture into colder regions of the world, and mass mortalities of cultured tilapia have been reported due to severe cold currents in winter. Since the late 1990s, several strains of Nile tilapia have been domesticated to improve the ability to adapt to low temperatures ... more

H.P. Zhu; Z.G. Liu; M.X. Lu; F.Y. Gao; X.L. Ke; Z.H. Huang
08/28/2015
Branchiostoma belcheri Gray; Conservation; FIASCO; Microsatellite

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed ... more

Z.B. Li; Y.S. Huang; J.B. Shangguan; Y.F. Ning; Y. Yi; G. Dai
08/10/2015
Genetic distance; Genetic diversity; Genetic similarity; Genetic structure; Microsatellite; Siniperca chuatsi

In order to provide genetic information for the selective breeding of Siniperca chuatsi, 14 microsatellite DNA loci were used to evaluate the genetic diversity and structure of four farmed populations and one wild population in China. The four cultivated populations were Foshan (FS), Jiangmen (JM), Nanjing (NJ), and Hongze Lake (HZL), and the wild population was collected from the ... more

C.F. Sun; X. Ye; Y.Y. Tian; J.J. Dong

Pages