Research Article

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Animals; Female; Forkhead Box Protein L2; Forkhead Transcription Factors; Gene expression; Goats; Hair follicle; Quantitative trait loci; Real-time polymerase chain reaction; Toll-Like Receptors; Wnt4 Protein

The mechanism of dominant follicle selection is unclear because of its physiological complexity. However, some studies have reported that the immune system plays an important role in reproductive physiology. The objective of the current study was to investigate the differential expression of Toll-like receptors (TLRs) in the dominant (DFs) and nondominant follicles (NFs), and to determine the ... more

G.X. E; Y.J. Zhao; Y.H. Ma; M.X. Chu; Q.H. Hong; S.J. Lv; L. Zhu; F.K. Li; Y.G. Han; X. Wu; Y.F. Huang
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Animals; Chromosome Mapping; Chromosomes, Mammalian; DNA, Mitochondrial; Evolution, Molecular; Genome; Genome, Mitochondrial; Goats; Mutagenesis, Insertional

Nuclear mitochondrial pseudogenes (numts), originating from mtDNA insertions into the nuclear genome, have been detected in many species. However, the distribution of numts in the newly published nuclear genome of domestic goat (Capra hircus) has not yet been explored. We used the entire goat mtDNA sequence and nuclear genome, to identify 118 numts using BLAST. Of these, 79 were able to map ... more

F.Y. Ning; J. Fu; Z.H. Du
3/15/2017
Animals; Breeding; Cells, Cultured; Female; Gene expression profiling; Gene Expression Regulation; Goats; Oocytes; Oogenesis; Single-Cell Analysis; Transcriptome

Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as ... more

X.Y. Yin; G.H. Cheng; H.Y. Guo; Q. Wang; Y.J. Li; H. Zhang
3/30/2017
Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo
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Animals; DNA, Mitochondrial; Goats; Haplotypes; Polymorphism, Genetic

The success of the geographical distribution of goat populations around the world is a consequence of the adaptive potential of these breeds. Several relevant traits to the success of the species in colonizing different ecosystems (and use by man) evolved before domestication. These features were relevant for the selection of different breeds. Each breed represents a genetic heritage that may ... more

N.M.V. Silva; E.C.Pimenta Filho; J.K.G. Arandas; M.A.Gomes Filho; E. Ferreira; I. Del Cerro; C. Fonseca; M.N. Ribeiro
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CART gene; Goats; Microsatellites; Reproduction traits; SNPs

Polymorphisms of the CART gene were investigated by PCR-single-strand conformation polymorphism analysis in 540 samples from 10 goat breeds. Ten novel single-nucleotide polymorphisms as well as three microsatellites were detected; a mutation, 77T→C, led to an amino acid change (Leu→Ser). Associations between polymorphic loci and reproductive traits were analyzed in Chuandong White, ... more

P.Q. Wang; L.M. Deng; B.Y. Zhang; M.X. Chu; J.Z. Hou
08/06/2012
Caseous lymphadenitis; Corynebacterium pseudotuberculosis; ERIC-PCR; Goats

Corynebacterium pseudotuberculosis, the infectious agent of caseous lymphadenitis (CLA), is responsible for substantial economic losses in goat and sheep production. Molecular characterization of C. pseudotuberculosis isolates by enterobacterial repetitive intergenic consensus (ERIC)-PCR has shown promising results in genotyping strains isolated from sheep with CLA. We ... more

E.M.S. Dorneles; J.A. Santana; G.I. Andrade; E.L.S. Santos; A.S. Guimarães; R.A. Mota; A.S. Santos; A. Miyoshi; V. Azevedo; A.M.G. Gouveia; A.P. Lage; M.B. Heinemann
08/25/2014
Goats; Parathyroid hormone-related peptide; Polyclonal antibody; Prokaryotic expression; Tissue expression profile

Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in Escherichia coli. Anti-PTHrP polyclonal antibody was ... more

H.L. Zheng; H. Li; Y.S. Sun; Z.Y. Yang; Q. Yu
06/24/2016
Ethnicity; Hazara Division; mtDNA; Pakistan; Phylogenetics

Mitochondrial DNA (mtDNA) is an important tool used to explore ethnogenetics and the evolutionary history of human populations. In this study, hypervariable segment I (HVSI) from mtDNA was analyzed to establish the genetic lineage of the Hazarewal populations residing in the Mansehra and Abbottabad districts of Northern Pakistan. HVSI was extracted from genetic specimens obtained from 225 ... more

N. Akbar; H. Ahmad; M.S. Nadeem; B.E. Hemphill; K. Muhammad; W. Ahmad; M. Ilyas; N. Akbar; H. Ahmad; M.S. Nadeem; B.E. Hemphill; K. Muhammad; W. Ahmad; M. Ilyas
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DNA Primers; DNA, Plant; Genetic markers; Genetic variation; Mangifera; Microsatellite Repeats; Pakistan; Phylogeny; Plant breeding; Plant Leaves; Polymorphism, Genetic

Understanding the genetic diversity of different Pakistani mango varieties is important for germplasm management and varietal characterization. Microsatellites are efficient and highly polymorphic markers for comparative genome mapping, and were used in the present study to determine the genetic relatedness and variability among 15 indigenous mango cultivars (Mangifera indica L.). Overall, 181 ... more

T. Nazish; G. Shabbir; A. Ali; S. Sami-Ul-Allah; M. Naeem; M. Javed; S. Batool; H. Arshad; S.B. Hussain; K. Aslam; R. Seher; M. Tahir; M. Baber

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