Research Article

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Natural resistance-associated macrophage protein gene 1 (Nramp1) plays an important role in the innate immune response of swine, and is believed to influence disease resistance. In this study, a real-time quantitative polymerase chain reaction technique was used to investigate Nramp1 expression in 12 different tissues in newborn and 7-, 14-, 21-, 28-, and 35-day-old Meishan piglets. Results ... more

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Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed males (GIFT strain) with an ... more

E.L. Buck; I.Y. Mizubuti; A.A. Alfieri; R.A.A. Otonel; L.Y. Buck; F.P. Souza; O.P. Prado-Calixto; A.R. Poveda-Parra; A. Filho; N.M. Lopera-Barrero
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Animals; Aquaculture; Cichlids; Fish Proteins; Gene expression; Gene Expression Regulation, Developmental; Muscle, Skeletal; Myostatin; Ponds

Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varieties were reared for 204 ... more

E.C.S. Lima; J.A. Povh; R.A.A. Otonel; J.H. Leonhardt; A.A. Alfieri; S.A. Headley; F.P. Souza; A.R. Poveda-Parra; P.J. Furlan-Murari; N.M. Lopera-Barrero
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Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varieties were reared for 204 ... more

E.C.S. Lima; J.A. Povh; R.A.A. Otonel; J.H. Leonhardt; A.A. Alfieri; S.A. Headley; F.P. Souza; A.R. Poveda-Parra; P.J. Furlan-Murari; N.M. Lopera-Barrero
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Alendronate regulates the activity of osteoclasts and healing of osteoporosis. This study investigated the effect of alendronate on bone healing and changes in the levels of cytokines. Bilateral ovaries of 10 adult female rabbits were removed surgically in aseptic condition to establish the animal model of osteoporosis. Five rabbits in group A were treated with alendronate (1.15 mg·kg·week) ... more

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The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum ethanolic extract (MPEE) on 1,2 dimethylhydrazine (DMH)-induced colorectal carcinogenesis in mice. Forty-two male Swiss mice (Mus musculus) were subdivided into six groups (N = 7/group): negative control, DMH, MPEE, pre-treatment, simultaneous, and post-treatment. Results showed that MPEE has ... more

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Type 2 diabetes mellitus (T2-DM) is a chronic metabolic disorder characterized by high blood glucose levels. T2-DM patients suffer from many complications, such as diabetic fatty liver and diabetic nephropathy. The liver, the pivotal organ involved in both glucose and lipid metabolism, is primarily damaged in T2-DM patients, especially in those with high levels of blood lipid. In this study, ... more

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The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo
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Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six ... more

I.S.B. Pinto; I. Fonseca; H.M. Brandão; J.C. Gern; A.S. Guimarães; W.A. Carvalho; M.A.V.P. Brito; L.F. Viccini; M.F. Martins

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