Research Article

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Low-cost and low-density (LCD) DNA arrays offer an
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A. Gaber; H. Hamed; E. Elsawy
03/30/2020
Brazil; E6/E7 genes; Focal epithelial hyperplasia; HPV-13; Human papillomavirus; Polymerase chain reaction

Human papillomavirus (HPV) type 13 is associated with focal epithelial hyperplasia, a benign and self-limited disease of oral mucosa. It is a rare finding, most often seen among young American natives. We carried out a molecular characterization case study of HPV type 13 isolated from a 14-year-old native girl living in Amazonia, in the northern region of Brazil, ... more

C.M. Borborema-Santos; N.Sde Lima Freitas; P.J.B. Santos; R.A. Barbosa-Filho; E. Costa-Lira; S. Talhari; S. Astolfi-Filho
10/02/2015
Cotton; Disease; DNA; Fusarium; Mexicali valley

Cotton production in the Mexicali valley is adversely affected by wilt and root rot disease associated with Fusarium species. In the present study, we sought to isolate and identify the Fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the Mexicali valley. Our analyses isolated four native fungi from the rhizosphere of cotton ... more

T. Gonzalez-Soto; D. González-Mendoza; R. Troncoso-Rojas; A. Morales-Trejo; C. Cecena-Duran; A. Garcia-Lopez; O. Grimaldo-Juarez
03/04/2016
Asian population; DNA; Genetics; Genome-wide association study; Venous thromboembolism

Venous thromboembolism (VTE) is a multifactorial disorder involving both acquired and genetic risk factors. The common genetic factors in Western populations have been studied and reported for several decades, while studies on Asian populations are relatively scarce. Evidence suggests that the prevalence and genetic risk factors of VTE vary significantly among ethnic populations. In this ... more

S.S. Huang; Y. Liu; Z.C. Jing; X.J. Wang; Y.M. Mao; S.S. Huang; Y. Liu; Z.C. Jing; X.J. Wang; Y.M. Mao
03/30/2017
Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo
04/05/2017
Alleles; DNA; DNA Primers; Forensic genetics; Gene frequency; Genetics, Population; Humans; Microsatellite Repeats; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Polymorphism, Genetic

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the ... more

R.G. Rodovalho; E.L. Rodrigues; G.S. Santos; L.M. Cavalcanti; P.R. Lima; A.G. Rodovalho; R.G. Vital; M.A.D. Gigonzac; A.D. da Cruz
10/05/2018
DNA; miRNA; mRNA; Next-generation sequencing; Phalaenopsis; PhalDB

The orchid family Orchidaceae is one of the largest families of angiosperms; it includes approximately 35,000 species, displays a wide range of unique flower shapes and is a valued ornamental crop. Among the various species of orchids, the Phalaenopsis genus is the most widely commercialized among blooming potted plants. Our aim was to provide in-depth ... more

C.Y. Lee; K.K. Viswanath; J.Z. Huang; C.P. Lee; C.P. Lin; T.C. Cheng; B.C. Chang; S.W. Chin; F.C. Chen
03/28/2019
barcodes; Desert plant; DNA; ITS2; matK; rbcL

Many of the plants that grow in arid and semi-arid regions under harsh conditions (drought, salinity and misuse) are of medical importance. Desert plants also provide other benefits; so conservation efforts should be strengthened in these areas. In this study, three DNA barcodes – chloroplast maturase-K (matK), ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL ... more

W.S. Al-Juhani
08/14/2019
CTAB; DNA; Mate; Molecular studies; Silica gel

Mate (Ilex paraguariensis) leaves are popular for consumption as an infusion, which provides various health benefits due to its nutraceutical properties. Leaf samples oxidize after harvesting, requiring special handling to avoid DNA damage or degradation by enzymatic or oxidative activities. The objectives of this work were to evaluate several methods for ... more

K.H. Lencina; J.M.B. Freitas; L. Essi; N. Pimentel; D.A. Bisognin
07/14/2015
Epigenetics; High-resolution melting; Imprinted genes; Methylation; Polymerase chain reaction

High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, primers that include CpG site(s) and ... more

F.V.M. Rubatino; N.V. Carobin; M.L. Freitas; V.T. de Oliveira; R.X. Pietra; P.P.R. Oliveira; A.A. Bosco; F.S. Jehee

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