Research Article

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05/11/2010
Dreb gene; Drought; Gene expression; Glycine max; Quantitative PCR; Water deficit

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. ... more

R. Stolf-Moreira; M.E. Medri; N. Neumaier; N.G. Lemos; R.L. Brogin; F.C. Marcelino; M.C.N. de Oliveira; J.R.B. Farias; R.V. Abdelnoor; A.L. Nepomuceno
03/31/2003
Double-stranded RNA (dsRNA); Gene expression; RNA interference; Trypanosoma

Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramming of genetic expression. Using primarily Trypanosoma ... more

S.M.R. Teixeira; W.D. da Rocha
10/04/2007
Co-regulation; Escherichia coli; Gene expression; Microarray analysis; Partial correlation; Transcriptional regulation

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide low-order partial correlation analysis of E. coli microarray data with the purpose of recovering regulatory ... more

D.F.T. Veiga; F.F.R. Vicente; M. Grivet; A. de la Fuente; A.T.R. Vasconcelos
03/31/2006
Bayesian bootstrap; Bioinformatics; Gene expression; Microarray; Statistics; Web tool

One of the goals of gene expression experiments is the identification of differentially expressed genes among populations that could be used as markers. For this purpose, we implemented a model-free Bayesian approach in a user-friendly and freely available web-based tool called BayBoots. In spite of a common misunderstanding that Bayesian and model-free approaches are incompatible, ... more

R.Z.N. Vêncio; D.F.C. Patrão; C.S. Baptista; C.A.B. Pereira; B. Zingales
09/30/2005
Feature selection; Gene expression; Genetic algorithms; Microarrays; Multi-class SVM

Microarrays are a new technology that allows biologists to better understand the interactions between diverse pathologic state at the gene level. However, the amount of data generated by these tools becomes problematic, even though data are supposed to be automatically analyzed (e.g., for diagnostic purposes). The issue becomes more complex when the expression data involve multiple ... more

B.Feres de Souza; A.Ponce de L. de Carvalho
10/14/2008
alpha-major regulatory element; Gene expression; globin genes; Hemoglobin; HS-40; thalassemia

Hemoglobin and globin genes are important models for studying protein and gene structure, function and regulation. We reviewed the main aspects of regulation of human α-globin synthesis, encoded by two adjacent genes (α2 and α1) clustered on chromosome 16. Their expression is controlled mainly by a regulatory element located 40 kb upstream on the same chromosome, the α- ... more

D.M. Ribeiro; M.F. Sonati
11/22/2011
b-actin; DEP chip; Electrochemical biosensor; Gene expression; Hoechst 33258; RAGE

An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of β-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak current, and the results were compared with conventional agarose ... more

P. Kittimongkolsuk; T. Tencomnao; R. Santiyanont
12/08/2011
Drought stress tolerance; Gene expression; Real-time PCR; Soybean; Transcription factor

To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed ... more

S.S. Pereira; F.C.M. Guimarães; J.F.C. Carvalho; R. Stolf-Moreira; M.C.N. Oliveira; A.A.P. Rolla; J.R.B. Farias; N. Neumaier; A.L. Nepomuceno
11/10/2011
Abiotic stress; Aluminum; Gene expression; MicroRNAs

MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe damage to plants. Although physiological aspects of aluminum ... more

J.C. Lima; R.A. Arenhart; M. Margis-Pinheiro; R. Margis
10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta

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