Research Article

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2017 May 04
DNA methylation; Nucleic Acid Amplification Techniques; Polymorphism, Genetic; Saccharum

Micropropagation is an important tool for large-scale multiplication of plant superior genotypes. However, somaclonal variation is one of the drawbacks of this process. Changes in DNA methylation have been widely reported as one of the main causes of somaclonal variations in plants. In order to investigate the occurrence of changes in the methylation pattern of sugarcane somaclonal variants, ... more

J.H.M.B. Francischini; E.L. Kemper; J.B. Costa; J.R.V. Manechini; L.R. Pinto
2017 May 10
Alleles; Animals; Expressed sequence tags; Genetic markers; Heterozygote; Microsatellite Repeats; Perciformes; Polymorphism, Genetic; Transcriptome

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

P.P. Wang; M. Ren; S.Q. Chen; S.W. Yin; C. Zhao; H.Y. Zhang; X.R. Li; Q.Q. Cao; G.Q. Zhou
2017 May 10
Alleles; Anacardiaceae; Genetic markers; Microsatellite Repeats; Plant breeding; Polymorphism, Genetic; Trees

Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associated DNA sequencing (RAD-seq) method and examined their ... more

Z.F. Wang; H.L. Cao; L.F. Wu; Y. Guo; Q.M. Mei; M. Li; Y. Wang; Z.M. Wang
2017 May 10
Animals; Ecosystem; Gene flow; Genetic Speciation; Karyotype; Perciformes; Polymorphism, Genetic; RNA, Ribosomal

The distribution patterns of marine biodiversity are complex, resulting from vicariant events and species dispersion, as well as local ecological and adaptive conditions. Furthermore, the wide geographic distribution of some species may be hindered by biogeographical barriers that can interfere in the gene flow. Cytogenetic analyses in marine fishes, especially those involving populations in ... more

K.D.J. Amorim; M.B. Cioffi; L.A.C. Bertollo; R.X. Soares; L.L. Calado; A.T. Borges; G.W.W.F. Costa; W.F. Molina
2017 May 18
Ecosystem; Genotype; Phenotype; Plant breeding; Polymorphism, Genetic; Quantitative Trait, Heritable; Selection, Genetic; Vigna

Estimating genetic parameters in plant breeding allows us to know the population potential for selecting and designing strategies that can maximize the achievement of superior genotypes. The objective of this study was to evaluate the genetic potential of a population of 20 cowpea genotypes by estimating genetic parameters and path analysis among the traits to guide the selection strategies. ... more

K.V. Lopes; P.E. Teodoro; F.A. Silva; M.T. Silva; R.L. Fernandes; T.C. Rodrigues; T.C. Faria; A.M. Corrêa
2017 May 18
Croton; Microsatellite Repeats; Polymorphism, Genetic

Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of ... more

C.S. Almeida-Pereira; A.V.C. Silva; R.P. Alves; R.B. Feitosa-Alcantara; M.F. Arrigoni-Blank; S.V. Alvares-Carvalho; T.S. Costa; L.A.S. White; V.S. Pinto; T.S. Sampaio; A.F. Blank
2017 May 18
Jatropha; Microsatellite Repeats; Polymorphism, Genetic

Physic nut (Jatropha curcas L.) presents high oilseed yield and low production cost. However, technical-scientific knowledge on this crop is still limited. This study aimed to evaluate and estimate the genetic variability of hybrids obtained from dialell crossing. Genetic variability was carried out using ISSR molecular markers. For genetic variability, nine primers were used, and six were ... more

D.O. Ribeiro; R. Silva-Mann; S.V. Alvares-Carvalho; E.M.S. Souza; M.C. Vasconcelos; A.F. Blank
2017 May 18
Animals; DNA, Mitochondrial; Goats; Haplotypes; Polymorphism, Genetic

The success of the geographical distribution of goat populations around the world is a consequence of the adaptive potential of these breeds. Several relevant traits to the success of the species in colonizing different ecosystems (and use by man) evolved before domestication. These features were relevant for the selection of different breeds. Each breed represents a genetic heritage that may ... more

N.M.V. Silva; E.C.Pimenta Filho; J.K.G. Arandas; M.A.Gomes Filho; E. Ferreira; I. Del Cerro; C. Fonseca; M.N. Ribeiro
2017 May 25
Animals; Caseins; Cattle; Female; Milk; Polymorphism, Genetic

Several human health problems have been related to the allergenic constitution of bovine milk due to the body's immune reaction to milk proteins. It is necessary find solutions to minimize the occurrence of such reactions, given the importance of milk as a source of animal protein. The aim of this study was to evaluate the allelic frequency of the CSN2 gene and to evaluate differences in the ... more

A.H.N. Rangel; L.G. Zaros; T.C. Lima; L.H.F. Borba; L.P. Novaes; L.F.M. Mota; M.S. Silva
2017 May 25
Alleles; Droughts; Genotype; Gossypium; Microsatellite Repeats; Polymorphism, Genetic; Stress, Physiological

Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (SSR) primers were used to estimate genetic divergence among 22 cotton ... more

A. Javaid; F.S. Awan; F.M. Azhar; I.A. Khan