Research Article

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This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the ... more

R.G. Rodovalho; E.L. Rodrigues; G.S. Santos; L.M. Cavalcanti; P.R. Lima; A.G. Rodovalho; R.G. Vital; M.A.D. Gigonzac; A.D. da Cruz
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DNA Primers; DNA, Plant; Genetic markers; Genetic variation; Mangifera; Microsatellite Repeats; Pakistan; Phylogeny; Plant breeding; Plant Leaves; Polymorphism, Genetic

Understanding the genetic diversity of different Pakistani mango varieties is important for germplasm management and varietal characterization. Microsatellites are efficient and highly polymorphic markers for comparative genome mapping, and were used in the present study to determine the genetic relatedness and variability among 15 indigenous mango cultivars (Mangifera indica L.). Overall, 181 ... more

T. Nazish; G. Shabbir; A. Ali; S. Sami-Ul-Allah; M. Naeem; M. Javed; S. Batool; H. Arshad; S.B. Hussain; K. Aslam; R. Seher; M. Tahir; M. Baber
2017 Apr 13
Asteraceae; Genotype; Microsatellite Repeats; Phylogeny; Polymorphism, Genetic

Yacon cultivation has been intensified and the investigations of this crop have increased at the scientific, agricultural, and social levels because the roots of yacon show beneficial properties for human health, such as reducing cholesterol and glucose blood levels. Since the investigations involving yacon are very recent, there is little information available in terms of the genetic ... more

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Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) ... more

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The Japanese oak silkmoth, Antheraea yamamai Guérin-Méneville, 1861 (Lepidoptera: Saturniidae), is an important natural resource of industrial value for silk fiber production. Owing to a lack of geographic and population genetic information, systematic domestication of An. yamamai has not been possible yet. In this study, 10 microsatellite markers developed using next-generation sequencing and ... more

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The objective of this article was to develop TRAP (target region amplification polymorphism) primers for castor bean, with the goal of making functional markers available for genetic studies about the species. To do this, oligonucleotides were designed based on ESTs, obtained from the NCBI (National Center for Biotechnology Information) databank, which code enzymes involved in metabolic routes ... more

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Animals; Gene frequency; Heterozygote; Humans; Polymorphism, Genetic; Software

The gene diversity or expected heterozygosity (H) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of H can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a ... more

A.R. da Silva; G. Malafaia; I.P.P. Menezes
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Alleles; Biodiversity; Brazil; Bromeliaceae; Forests; Genetic Loci; Inbreeding; Polymorphism, Genetic

The Bromeliaceae family includes a range of species used for many purposes, including ornamental use and use as food, medicine, feed, and fiber. The state of Espírito Santo, Brazil is a center of diversity for this family in the Atlantic Forest. We evaluated the genetic diversity of five populations of the Bromeliaceae family, including specimens of the genera Aechmea, Billbergia (subfamily ... more

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The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered an important plant genetic resource. Here, we report the development ... more

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Alleles; Animals; Cyprinidae; Expressed sequence tags; Gene Transfer, Horizontal; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic

We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuciscus baicalensis populations in Xinjiang. There were 4-27 (average ... more

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