Research Article

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Amino acids; Animals; Collagen; Fish Proteins; Gadiformes; Protein Denaturation; Protein Subunits; Skin

Acid and pepsin-soluble collagen (ASC and PSC, respectively) were extracted from cod (Gadus macrocephaius) skin, and yields of 37.36 and 55.96% were obtained for ASC and PSC, respectively. The total yield of ASC and PSC was 93.92%, based on the lyophilized dry weight, which is higher than that obtained from other sources. Electrophoresis revealed that both ASC and PSC consisted of two ... more

Y. Shu; H. Ren; R. Ao; W.C. Qi; Z.S. Zhang
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Animals; Blastocyst; Carps; Fish Proteins; Fructose-Bisphosphate Aldolase; Gene Expression Regulation, Developmental; Organ Specificity

Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model owing to its relatively high ... more

J.J. Fan; J.J. Bai; D.M. Ma; L.Y. Yu; P. Jiang
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Cadmium; Cloning; Metallothionein-2; Pheretima aspergillum (E. Perrier)

Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. aspergillum, analyzed its ... more

L. Gong; W. Li; J. Li; W.E. Li; W.R. Wu; L.W. Yu
10/27/2015
Cadmium; Genetic variability; Solanum lycopersicum; Tomato

Due to increased global concern over the deleterious effects of toxic heavy metals in the environment, it has become necessary to develop plant genotypes that limit the uptake of heavy metals to aerial edible parts. To address this concern, we performed a glasshouse experiment to assess variations within tomato germplasm for cadmium (Cd) tolerance under control conditions and under ... more

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Hepatocellular carcinoma; Metallothionein; RNA sequencing

According to the typical clinical characteristics of hepatocellular carcinoma (HCC), recurrence and prognosis can differ dramatically between patients. Using RNA sequencing, we identified differential expression of the gene metallothionein 1M (MT1M) by comparing early-recurrence HCC (N = 11), no-recurrence HCC (N = 10), and normal liver tissues (N = 5). Reverse transcription- ... more

J. Ding; S.C. Lu; J. Ding; S.C. Lu
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Adaptation, Physiological; Copper; Gene Expression Regulation, Plant; Metallothionein; Plant Leaves; Plant Proteins; Prosopis; Time Factors

For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous to the MT type 2 (isoform 1), Mt2, and Mt3 sequences of other plant ... more

C. Michel-Lopez; O. Zapata-Pérez; D. González-Mendoza; O. Grimaldo-Juarez; C. Ceceña-Duran; O. Tzintzun-Camacho
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Breed; Chicken FTO; Hypothalamus; Liver; Skeletal muscle

The fat mass- and obesity-associated gene (FTO) is involved in energy metabolism, but little is known about the chicken FTO gene. The objective of the current study was to detect chicken FTO expression patterns in the hypothalamus, liver, and skeletal muscle during development, and analyze the effects of age and breed on FTO expression. Real-time quantitative polymerase chain reaction results ... more

C. Song; W.T. Song; J.T. Shu; Z.Y. Tao; W.Q. Zhu; C. Di; H.F. Li
12/21/2015
Akirin2 expression; Bursa; Chicken; Immune response; Liver

Akirin2 is a nuclear factor that plays an important role in the development and regulation of innate immune response. In this study, akirin2 gene expression in several primary immune organs (liver, thymus, and bursa) of Hi-Line Brown chicken administered with the LoSota vaccine was analyzed during the various stages of increase in Newcastle disease virus antibody titer. The ... more

C.L. Man; W.T. Mu; Y. Chang; D.X. Zhao
12/08/2015
Chemical treatment; Dendritic cells; Immune treatment; Liver; Metastasis; Recurrence

We aimed to evaluate dendritic cell (DC) tumor vaccines for preventing liver cancer recurrence and metastasis. DCs were induced from mononuclear cells in the peripheral blood with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4), followed by sensitization with lysis of autologous liver cancer cells. One hundred and ... more

T.Y. Sun; W. Yan; C.M. Yang; L.F. Zhang; H.L. Tang; Y. Chen; H.X. Hu; X. Wei
07/25/2016
Antioxidant enzymes; Environmental static electric field; Liver

With the increasing voltage of direct current transmission line, the intensity of the environmental static electric field has also increased. Thus, whether static electric fields cause biological injury is an important question. In this study, the effects of chronic exposure to environmental static electric fields on some antioxidant enzymes activities in the hepatocytes of mice were ... more

S.X. Wu; Y.Q. Xu; G.Q. Di; J.H. Jiang; L. Xin; T.Y. Wu; S.X. Wu; Y.Q. Xu; G.Q. Di; J.H. Jiang; L. Xin; T.Y. Wu

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