Research Article

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08/31/2017
Fabaceae; Phylogeny; Polymorphism, Genetic

Poincianella pyramidalis (Tul.) L.P. Queiroz is an endemic Caatinga (Brazilian savannah biome) species that has been exploited for different purposes, although information is necessary about still existing natural populations. The objective of this study was to evaluate the genetic diversity among 20 P. pyramidalis individuals occurring in a population localized in the Caatinga biome of ... more

K.S. Belarmino; M.M. Rêgo; R.L.A. Bruno; G.D.A. Medeiros; A.P. Andrade; E.R. Rêgo
09/21/2017
Animals; Brazil; Dogs; Feces; Genome, Viral; Parvoviridae Infections; Parvovirus, Canine; Phylogeny; Polymorphism, Genetic; Recombination, Genetic

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
09/21/2017
Arachis; Genome, Plant; Karyotype; Phylogeny; Polymorphism, Genetic; Principal component analysis

Arachis kuhlmannii occurs in Mato Grosso and Mato Grosso do Sul States, Brazil. Its area of occurrence partially overlaps with that of other species in the Arachis section. Because of their morphological similarities, these species are often mistaken one for another. This study aimed the correct classification of available accessions as Arachis kuhlmannii, or other species, and the ... more

A.P. Fávero; A.P.S. Peñaloza; C.R. Lopes; J.F.M. Valls
12/19/2016
cluster analysis; Cold Temperature; DNA, Plant; Genetic markers; Microsatellite Repeats; Polymorphism, Genetic; Stress, Physiological; Zea mays

In this study, eight inbred maize lines with high or low tolerance to cold temperature were investigated using bulk segregant analysis (BSA). Genetic diversity and genetic relationships were investigated using 100 simple sequence repeat (SSR) markers linked to cold tolerance in maize. A total of 319 alleles were identified. Among these, 128 were high coldtolerant-specific alleles and 61 were ... more

M.Q.U. Farooqi; K.J. Sa; T.K. Hong; J.K. Lee
1/23/2017
Anacardiaceae; Genetic markers; Genetic variation; Genetics, Population; Genotype; Microsatellite Repeats; Polymorphism, Genetic

Cajazeira (Spondias mombin L.), of the family Anacardiaceae, is a species of fruit tree found in the Amazon region with fruits that have excellent prospects for commercial use. We aimed to evaluate the genetic diversity within and among natural populations of S. mombin, with natural occurrence in northern Mato Grosso State, by using inter-simple sequence repeat (ISSR) markers. Overall, 126 ... more

B.M. Silva; A.A.B. Rossi; A.V. Tiago; K.F.M. Schmitt; J.F.E. Dardengo; S.A.M. Souza
2/08/2017
Alleles; Animals; Bivalvia; Evolution, Molecular; Genetics, Population; Heterozygote; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic

Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp and the number of alleles per locus varied between 2 and 23. ... more

Y.W. Feng; H.L. Jiang; Y.T. Liu; X.P. Wu; X.Q. Liu; X.M. Wei
3/08/2017
Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
4/05/2017
Alleles; DNA; DNA Primers; Forensic genetics; Gene frequency; Genetics, Population; Humans; Microsatellite Repeats; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Polymorphism, Genetic

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the ... more

R.G. Rodovalho; E.L. Rodrigues; G.S. Santos; L.M. Cavalcanti; P.R. Lima; A.G. Rodovalho; R.G. Vital; M.A.D. Gigonzac; A.D. da Cruz
4/13/2017
Animals; Bombyx; DNA, Mitochondrial; Gene Pool; Microsatellite Repeats; Polymorphism, Genetic

The Japanese oak silkmoth, Antheraea yamamai Guérin-Méneville, 1861 (Lepidoptera: Saturniidae), is an important natural resource of industrial value for silk fiber production. Owing to a lack of geographic and population genetic information, systematic domestication of An. yamamai has not been possible yet. In this study, 10 microsatellite markers developed using next-generation sequencing and ... more

S.R. Kim; K.Y. Kim; J.S. Jeong; M.J. Kim; K.H. Kim; K.H. Choi; I. Kim
4/28/2017
Dipteryx; Gene frequency; Genetic markers; Genotype; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic; Sequence Analysis, DNA

The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered an important plant genetic resource. Here, we report the development ... more

R.A. Guimarães; M.P.C. Telles; A.M. Antunes; K.M. Corrêa; C.V.G. Ribeiro; A.S.G. Coelho; T.N. Soares

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