Research Article

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2017 Mar 30
Abscisic acid; Adaptation, Physiological; Citrus; Dioxygenases; Droughts; Nicotiana; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Stress, Physiological

9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) ... more

A.M. Pedrosa; L.C. Cidade; C.P.S. Martins; A.F. Macedo; D.M. Neves; F.P. Gomes; E.I.S. Floh; M.G.C. Costa
05/25/2017
Disease resistance; Fusarium; Gene Expression Regulation, Plant; Plant Proteins; Plant Roots; Proteome; Solanum lycopersicum; Stress, Physiological

Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by ... more

T.D. Silva; C.M.A. Almeida; C.B. Malafaia; L.M.S. Oliveira; M.V. Silva; M.T.S. Correia
06/29/2017
Chromosomes, Plant; DNA methylation; Genome, Plant; Genome-wide association study; Gossypium; Salinity; Stress, Physiological

Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well understood. Here, we constructed a map of genome-wide DNA methylation ... more

X.K. Lu; N. Shu; J.J. Wang; X.G. Chen; D.L. Wang; S. Wang; W.L. Fan; X.N. Guo; L.X. Guo; W.W. Ye
08/31/2017
Amino Acid Motifs; Cloning, Molecular; Droughts; Hordeum; Plant Proteins; Protein domains; Qa-SNARE Proteins; Stress, Physiological

Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, "Himalaya 10"; its expression was evaluated during drought stress and rehydration via real-time PCR. The cloned HbSYR1 ... more

Q.J. Xu; Y.L. Wang; Z.X. Wei; H.J. Yuan; X.Q. Zeng; N. Tashi
1/23/2017
Animals; Lymphocytes; Mice; Oryza; Plant Proteins; Plants, Genetically Modified

With the expanding demand for genetically modified (GM) rice, its safety evaluation is of great significance. Therefore, this study was carried out to assess the acute cytotoxicity of the whole protein extract from GM rice Bar68-1 in Mus musculus lymphocytes in vitro. Cell viability was determined by Cell Counting Kit-8 (CCK-8) and Neutral Red Uptake (NRU) tests. CCK-8 tests was carried out ... more

X.B. Lu
06/29/2017
Actins; Aquaporins; Cold-Shock Response; Cyclophilins; Eukaryotic Initiation Factor-4A; Genes, Plant; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Oryza; Plant Proteins; Real-time polymerase chain reaction; Reference Standards; Tubulin; Ubiquitins

Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pathways and cellular compartments, resulting in general or specific ... more

P.A. Auler; L.C. Benitez; M.N. do Amaral; I.L. Vighi; G.S. Rodrigues; L.C. da Maia; E.J.B. Braga
05/13/2013
Central nervous system; Cytotoxicity; Iron; Micronuclei

Iron is the most important metallic chemical element on Earth. Poisoning caused by excessive iron in humans has been associated with pulmonary diseases including neoplasms caused by inhalation of iron oxides. The involvement of iron in neurodegenerative processes has already been described. DNA alterations are induced by iron and other chemical compounds containing this metal; however, ... more

D.D.F.A. Alcântara; H.F. Ribeiro; L.A. Matos; J.M.C. Sousa; R.R. Burbano; M.O. Bahia
12/17/2012
FRO genes; Iron; Medicago truncatula

Iron (Fe) is an essential element for plant growth. Commonly, this element is found in an oxidized form in soil, which is poorly available for plants. Therefore, plants have evolved ferric-chelate reductase enzymes (FRO) to reduce iron into a more soluble ferrous form. Fe scarcity in plants induce the FRO enzyme activity. Although the legume Medicago truncatula has been employed ... more

Mdel C. Orozco-Mosqueda; G. Santoyo; R. Farias-Rodriguez; L. Macías-Rodríguez; E. Valencia-Cantero
03/11/2016
Differentially expressed genes; MicroRNAs; RNA sequencing; Thyroid carcinoma; Transcription factors

In this study, we examined the molecular mechanism of thyroid carcinoma (THCA) using bioinformatics. RNA-sequencing data of THCA (N = 498) and normal thyroid tissue (N = 59) were downloaded from The Cancer Genome Atlas. Next, gene expression levels were calculated using the TCC package and differentially expressed genes (DEGs) were identified using the edgeR package. A co-expression ... more

J.C. Lu; Y.P. Zhang; J.C. Lu; Y.P. Zhang
11/26/2015
Epigenetics; Methylation; Methylation-sensitive amplified polymorphism; Nia1; Phenotype; Tobacco (Nicotiana tabacum)

This study aimed to investigate cytosine methylation profiles in different tobacco (Nicotiana tabacum) cultivars grown in China. Methylation-sensitive amplified polymorphism was used to analyze genome-wide global methylation profiles in four tobacco cultivars (Yunyan 85, NC89, K326, and Yunyan 87). Amplicons with methylated C motifs were cloned by reamplified polymerase chain ... more

J. Jiao; J. Wu; Z. Lv; C. Sun; L. Gao; X. Yan; L. Cui; Z. Tang; B. Yan; Y. Jia

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