Research Article

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2017 Sep 27
Gene-Environment Interaction; Genotype; Germination; Glycine max; Models, Genetic; Quantitative Trait, Heritable; seeds

Estimating genotype x environment (GxE) parameters for quality and yield in soybean seed grown in different environments in Minas Gerais State was the goal of this study, as well as to evaluate interaction effects of GxE for soybean seeds yield and quality. Seeds were produced in three locations in Minas Gerais State (Lavras, Inconfidentes, and Patos de Minas) in 2013/14 and 2014/15 seasons. ... more

E.V. Zambiazzi; A.T. Bruzi; S.R. Guilherme; D.R. Pereira; J.G. Lima; A.M. Zuffo; F.O. Ribeiro; A.E.S. Mendes; S.H.M. Godinho; M.L.M. Carvalho
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Phenotyping; Quality; seeds; Ward-MLM

Passion fruit is a native fruit of tropical America, with Brazil being the world's leading producer and consumer of this fruit, with an estimated annual production of 554,598 Mg, and yield of 13,500 kg.ha-1 Phenotyping through digital images has been used to evaluate morphological characteristics of seeds. Knowledge of the degree of genetic divergence ... more

L.R. Fachi; W. Krause; H.D. Vieira; D.V. Araújo; P.B. Luz; A.P. Viana
05/25/2017
Disease resistance; Fusarium; Gene Expression Regulation, Plant; Plant Proteins; Plant Roots; Proteome; Solanum lycopersicum; Stress, Physiological

Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by ... more

T.D. Silva; C.M.A. Almeida; C.B. Malafaia; L.M.S. Oliveira; M.V. Silva; M.T.S. Correia
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desiccation tolerance; high temperature; quantitative trait locus; seeds; Zea mays

Quantitative trait locus (QTL) mapping and identification of traits of agronomic importance is important in the process of molecular marker-assisted selection in breeding programs. The molecular map of maize is well saturated and QTL and simple sequence repeat (SSR) markers have been identified, whereas few markers linked to seed quality traits are included. The present study aimed to identify ... more

K.C.P.C. Salgado; E.V.R. Von Pinho; C.T. Guimarães; R.G. Von Pinho; C.A. Ferreira; V. Andrade
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Gene expression; Lignin; Quantitative real-time PCR; seeds; Soybean

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied ... more

A. Baldoni; E.V.R. Von Pinho; J.S. Fernandes; V.M. Abreu; M.L.M. Carvalho
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Apis cerana; Differential expression; Proteome; Varroa destructor

The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the proteomes ... more

T. Ji; F. Shen; Z. Liu; L. Yin; J. Shen; Q. Liang; Y.X. Luo
11/30/2015
Ecology; Flue-cured tobacco; Leaf quality; Proteome; Two-dimensional electrophoresis

The leaves of tobacco plants were used to analyze differences in protein content of tobacco grown in the four main flue-cured tobacco-producing areas of Sichuan Province, China. An improved protein extraction method, isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis two-dimensional gel electrophoretic separation, was used to extract and separate total protein from ... more

L.M. Lu; K.Y. Ye; Z.X. Tang; L. Liu; Y. Chen; J. Luo; Y.B. Huang
05/13/2016
Mass spectrometry; Mycelium; Nitrogen stress; Proteome; Rice blast fungus

Magnaporthe oryzae is an important model system in studies of plant pathogenic fungi, and nitrogen is a key nutrient source affecting microbial growth and development. In order to understand how nitrogen stress causes changes in mycelial proteins, we analyzed differentially expressed mycelial proteins from the M. oryzae virulent strain CH-63 using two-dimensional ... more

X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao; X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao
12/02/2016
Alcohol Oxidoreductases; Anthocyanins; Cloning, Molecular; Flavonols; Fruit; Gene Expression Regulation, Plant; Glucosyltransferases; Organ Specificity; Plant Proteins; Transcription factors; Vitis

MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and ... more

T.Q. Niu; Z.D. Gao; P.F. Zhang; X.J. Zhang; M.Y. Gao; W. Ji; W.X. Fan; P.F. Wen
2016 Dec 02
Alcohol Oxidoreductases; China; Cloning, Molecular; Eucalyptus; Gene Expression Regulation, Plant; Lignin; Nicotiana; Phylogeny; Plant Proteins; Plants, Genetically Modified

Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final step in lignin biosynthesis. The genus Eucalyptus belongs to the family Myrtaceae, which is the main cultivated species in China. Eucalyptus urophylla GLU4 (GLU4) is widely grown in Guangxi. It is preferred for pulping because of its excellent cellulose content and fiber length. Based on GLU4 and CAD gene expression, a Eucalyptus variety ... more

B.W. Chen; Y.F. Xiao; J.J. Li; H.L. Liu; Z.H. Qin; Y. Gai; X.N. Jiang

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