Research Article

Related GMR Articles

10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
07/24/2013
cDNA-AFLP; Floral bud abortion; Gene expression; Radish; Real-time PCR

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain ... more

J. Zhang; X.L. Sun; L.G. Zhang; M.X. Hui; M.K. Zhang
06/28/2013
BPI gene; Gene expression; Pig; Real-time PCR

We compared and analyzed the expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18 and 35-day expres­sion in the jejunum. There were also significant differences ... more

J. Zhu; C. Zi; Z.C. Wu; L. Liu; X.R. Zheng; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
08/28/2014
Fatty acid desaturase; Gene expression; Polyunsaturated fatty acids (PUFAs); Real-time PCR; Safflower

Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and CtFAD8 in flowers. This result indicates that CtFAD3 ... more

L.L. Guan; W. Wu; B. Hu; D. Li; J.W. Chen; K. Hou; L. Wang
02/28/2013
Gene expression; Lignin; Quantitative real-time PCR; seeds; Soybean

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied ... more

A. Baldoni; E.V.R. Von Pinho; J.S. Fernandes; V.M. Abreu; M.L.M. Carvalho
07/08/2013
Gene expression; Kidney; Promoter region; Transcription factor; WNK4

With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoal­dosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mechanism of WNK4 ... more

Z.J. Sun; Y. Li; Y.H. Li; J.S. Zhang; Q. Ding; J. Li-Ling; Y. Liang; Y.Y. Zhao
03/31/2003
Double-stranded RNA (dsRNA); Gene expression; RNA interference; Trypanosoma

Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramming of genetic expression. Using primarily Trypanosoma ... more

S.M.R. Teixeira; W.D. da Rocha
10/04/2007
Co-regulation; Escherichia coli; Gene expression; Microarray analysis; Partial correlation; Transcriptional regulation

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide low-order partial correlation analysis of E. coli microarray data with the purpose of recovering regulatory ... more

D.F.T. Veiga; F.F.R. Vicente; M. Grivet; A. de la Fuente; A.T.R. Vasconcelos
03/31/2006
Bayesian bootstrap; Bioinformatics; Gene expression; Microarray; Statistics; Web tool

One of the goals of gene expression experiments is the identification of differentially expressed genes among populations that could be used as markers. For this purpose, we implemented a model-free Bayesian approach in a user-friendly and freely available web-based tool called BayBoots. In spite of a common misunderstanding that Bayesian and model-free approaches are incompatible, ... more

R.Z.N. Vêncio; D.F.C. Patrão; C.S. Baptista; C.A.B. Pereira; B. Zingales
09/30/2005
Feature selection; Gene expression; Genetic algorithms; Microarrays; Multi-class SVM

Microarrays are a new technology that allows biologists to better understand the interactions between diverse pathologic state at the gene level. However, the amount of data generated by these tools becomes problematic, even though data are supposed to be automatically analyzed (e.g., for diagnostic purposes). The issue becomes more complex when the expression data involve multiple ... more

B.Feres de Souza; A.Ponce de L. de Carvalho

Pages