Research Article

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2017 Sep 21
Animals; Biomarkers, Tumor; Conserved sequence; Helminth Proteins; Protein Binding; Protein domains; Protein stability; Sequence Homology; Tumor Protein, Translationally-Controlled 1; Tylenchoidea

Plant parasitic nematodes infect a wide range of hosts representing the largest source of biotic stress experienced by plants. Meloidogyne genus comprises the most important parasitic nematodes, also known as root-knot nematodes. These parasitic organisms obtain nutrients to support their development through complex interactions with their hosts. The translationally-controlled tumor protein ( ... more

R.M.Moraes Filho; A.F. Menezes; L.S.S. Martins
Amino Acid Motifs; Cloning, Molecular; Droughts; Hordeum; Plant Proteins; Protein domains; Qa-SNARE Proteins; Stress, Physiological

Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, "Himalaya 10"; its expression was evaluated during drought stress and rehydration via real-time PCR. The cloned HbSYR1 ... more

Q.J. Xu; Y.L. Wang; Z.X. Wei; H.J. Yuan; X.Q. Zeng; N. Tashi
ABC transport; Oligopeptide-binding protein; Opp; Structural model; Xanthomonas

The oligopeptide-binding protein, OppA, ushers oligopeptide substrates to the membrane-associated oligopeptide permease (Opp), a multi-component ABC-type transporter involved in the uptake of oligopeptides by several bacterial species. In the present study, we report a structural model and an oligopeptide docking analysis of the OppA protein expressed by Xanthomonas axonopodis ... more

A. Moutran; A. Balan; L.C.S. Ferreira; A. Giorgetti; A. Tramontano; R.C.C. Ferreira
Corynebacterium; Genome annotation; Metabolic networks; Systems biology; Xanthomonas

New sequencing technologies provide ultra-fast access to novel microbial genome data. For their interpretation, an efficient bioinformatics pipeline that facilitates in silico reconstruction of metabolic networks is highly desirable. The software tool CARMEN performs in silico reconstruction of metabolic networks to interpret genome data in a functional context. CARMEN ... more

J. Schneider; F.J. Vorhölter; E. Trost; J. Blom; Y.R. Musa; H. Neuweger; K. Niehaus; S. Schatschneider; A. Tauch; A. Goesmann
Capsicum; Genes, Plant; Genetic variation; Plant Immunity; Xanthomonas

Since 2008, Brazil is the largest consumer of agrochemicals, which increases production costs and risks of agricultural products, environment, and farmers' contamination. Sweet pepper, which is one of the main consumed vegetables in the country, is on top of the list of the most sprayed crops. The bacterial spot, caused by Xanthomonas spp, is one of the most damaging diseases of pepper crops. ... more

L.R.A. Silva; R. Rodrigues; S. Pimenta; J.W.S. Correa; M.S.B. Araújo; C.S. Bento; C.P. Sudré
Ascomycota; Capsicum; Disease resistance; Fruit; Genotype; Models, Genetic; Plant breeding; Plant Diseases; Plant Leaves; Potyvirus; Quantitative Trait, Heritable; Selective breeding; Xanthomonas

This study aimed to identify Capsicum genotypes with resistance to bacterial spot (BS), anthracnose and Pepper yellow mosaic virus (PepYMV). Fifty-four genotypes of Capsicum spp were evaluated. Resistance reaction against BS was evaluated using three replicates, testing hypersensitivity and quantitative resistance in leaves. After evaluation, inoculated leaves were detached from the plants, ... more

C.S. Bento; A.G. de Souza; C.P. Sudré; S. Pimenta; R. Rodrigues
Chromobacterium violaceum; Genome; Pathogenicity; Virulence

Chromobacterium violaceum is a versatile, Gram-negative β-protebacterium that grows in a variety of ecosystems in tropical and subtropical areas, such as the water and borders of the Negro River, in the Amazon region of Brazil. Although it is a saprophyte and is generally considered non-pathogenic, sporadic cases of human infection have been described, mainly in young children and in ... more

C.F.Alves de Brito; C.M.B. Carvalho; F.R. Santos; R.T. Gazzinelli; S.C. Oliveira; V. Azevedo; S.M.R. Teixeira
Dimorphism; Host-pathogen interaction; Paracoccidioides brasiliensis; Pathogenicity; Transcriptome analysis; Virulence

Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, is a dimorphic fungus, which is found as mycelia at 22-26ºC and as yeasts at 37ºC. A remarkable feature common to several pathogenic fungi is their ability to differentiate from mycelium to yeast morphologies, or vice-versa. Although P. brasiliensis is a recognized pathogen for humans, ... more

A.Henrique Tavares; S.Souza Silva; V.Vilmar Bernardes; A.Queiróz Maranhão; A.Queiróz Maranhão; C.Maria Kyaw Kyaw; M. Poças-Fonseca; I. Silva-Pereira
Enzyme activity; Hyphal morphology; Molecular chaperones; Protein secretion; Virulence

Quantitative and qualitative relationships were found between secreted proteins and their activity, and the hyphal morphol­ogy of Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao. This fungus was grown on fermentable and non-fermentable carbon sources; significant differences in myce­lial morphology were observed and correlated ... more

F.C. Alvim; E.M. Mattos; C.P. Pirovani; K. Gramacho; C. Pungartnik; M. Brendel; J.C.M. Cascardo; M. Vincentz
Amino acid usage; Essential amino acid; Protein domains

Nutrigenomics studies the effects of nutrients on the genome, transcriptome and proteome of organisms, and here an evolutionary standpoint on this new discipline is presented. It is well known that metazoan organisms are unable to synthesize all amino acids necessary to produce their proteins and that these essential amino acids (EAA) must be acquired from the diet. Here, we tested the ... more

L. Santana-Santos; F. Prosdocimi; J.M. Ortega