Research Article

Related GMR Articles

4/05/2017
Alleles; DNA; DNA Primers; Forensic genetics; Gene frequency; Genetics, Population; Humans; Microsatellite Repeats; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Polymorphism, Genetic

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the ... more

R.G. Rodovalho; E.L. Rodrigues; G.S. Santos; L.M. Cavalcanti; P.R. Lima; A.G. Rodovalho; R.G. Vital; M.A.D. Gigonzac; A.D. da Cruz
12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada
4/05/2017
Animals; Brazil; Cattle; Cattle Diseases; Coronavirus Infections; Coronavirus, Bovine; Dysentery; Evolution, Molecular; Feces; Membrane Glycoproteins; Phylogeny; Sequence Analysis, DNA; Viral Envelope Proteins; Viral Tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of respiratory disease in ... more

E.A. Beuttemmuller; A.F. Alfieri; S.A. Headley; A.A. Alfieri
05/18/2017
Animals; Bacillus amyloliquefaciens; Escherichia coli; Feces; Microbiota; Murinae; Republic of Korea; RNA, Ribosomal, 16S; Serratia liquefaciens; Staphylococcus

Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and ... more

M.M. Rahman; K.B. Yoon; S.J. Lim; M.G. Jeon; H.J. Kim; H.Y. Kim; J.Y. Cho; H.M. Chae; Y.C. Park
09/21/2017
Animals; Brazil; Dogs; Feces; Genome, Viral; Parvoviridae Infections; Parvovirus, Canine; Phylogeny; Polymorphism, Genetic; Recombination, Genetic

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
12/02/2016
Administration, Oral; Animals; Bone marrow cells; Cell proliferation; Cells, Cultured; Guatemala; Humans; Lymphocytes; male; Mexico; Micronucleus tests; Mutagenicity Tests; Plant Extracts; Rats; Rubus; Sister chromatid exchange; Toxicity Tests, Subchronic

Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of ... more

S. González-Hernández; D. González-Ramírez; M.I. Dávila-Rodríguez; A. Jimenez-Arellanez; M. Meckes-Fischer; S. Said-Fernández; E.I. Cortés-Gutiérrez
12/02/2016
Animals; Citalopram; Dementia, Vascular; Disease Models, Animal; Dopamine; Gene Expression Regulation; Hippocampus; Humans; Injections, Intraperitoneal; male; Neurons; Norepinephrine; Random Allocation; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT1A; Selective Serotonin Reuptake Inhibitors; Serotonin; Treatment outcome

5-hydroxytryptamine receptor 1A (5-HT1AR) is closely associated with cognitive functions. Selective serotonin reuptake inhibitors (SSRIs) can protect individuals from brain damage following ischemia/hypoxia. To investigate the function of SSRIs in vascular dementia (VD), we established a rat model of VD, and observed the effect of SSRIs on the expression of 5-HT1AR mRNA and neurotransmitters. ... more

K. Guo; G. Yin; X.H. Zi; H.X. Zhu; Q. Pan
12/19/2016
Animals; Cell Line, Tumor; Cell Movement; Cell proliferation; Endoglin; Female; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Nude; MicroRNAs; Neoplasm Invasiveness; Neoplasm Transplantation; Neovascularization, Pathologic; Thyroid Neoplasms

Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in thyroid cancer cells. A wound healing assay was adopted to examine ... more

Y.L. Wang; W.G. Gong; Q.L. Yuan
12/19/2016
Animals; Astragalus propinquus; Biomarkers; Bone Density; Disease Models, Animal; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Female; Humans; Mice; Osteoporosis, Postmenopausal; Ovariectomy; Polysaccharides; Random Allocation; Signal transduction

Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on ... more

J. Huo; X. Sun
12/19/2016
Animals; Animals, Genetically Modified; Apoptosis Regulatory Proteins; Crosses, Genetic; Drosophila melanogaster; Drosophila Proteins; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Humans; male; Membrane Proteins; Neurons; Proto-Oncogene Proteins c-bcl-2; Sequence Homology, Amino Acid; Transgenes

Lifeguard is an integral transmembrane protein that modulates FasL-mediated apoptosis by interfering with the activation of caspase 8. It is evolutionarily conserved, with homologues present in plants, nematodes, zebra fish, frog, chicken, mouse, monkey, and human. The Lifeguard homologue in Drosophila, CG3814, contains the Bax inhibitor-1 family motif of unknown function. Downregulation of ... more

P.G. M'Angale; B.E. Staveley

Pages