Research Article

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4/20/2017
Ascomycota; Brazil; Ecosystem; Evolution, Molecular; Fungal Proteins; Mutation; Peptide Elongation Factor 1; Phylogeography; Polymorphism, Genetic; Selection, Genetic

Lasiodiplodia theobromae is a plant pathogen with a worldwide distribution, with low host specificity, causing stem cankers, dieback diseases, and fruit rot in several species of plants. In coconut, this pathogen is reported as the etiological agent of "coconut leaf blight" (CLB) disease, causing several losses in fruit production. The CLB is an important disease for this crop in Brazil. In ... more

P.H.D. Santos; B.M. Carvalho; K.P. Aguiar; F.A.S. Aredes; T.P.S. Poltronieri; J.M.S. Vivas; M. Dias; G.A. Bezerra; D.B. Pinho; M.G. Pereira; S.F. Silveira
05/10/2017
Animals; Brazil; Cats; Feline Acquired Immunodeficiency Syndrome; Female; Immunodeficiency Virus, Feline; Leukemia Virus, Feline; Leukemia, Feline; male; Pets

Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial ... more

L.C. Lacerda; A.N. Silva; J.S. Freitas; R.D.S. Cruz; R.A. Said; A.D. Munhoz
05/18/2017
Archaea; Bacteria; Brazil; Lakes; Microbiota; Rivers; RNA, Ribosomal, 16S

The Amazon region has the largest hydrographic basin on the planet and 
is well known for its huge biodiversity of plants and animals. However, 
there is a lack of studies on aquatic microbial biodiversity in the 
Solimões River, one of its main water courses. To investigate the 
microbial biodiversity of this region, we performed 16S rRNA gene clone 
libraries from Solimões River and adjacent ... more

D. Toyama; C.D. Santos-Júnior; L.T. Kishi; T.C.S. Oliveira; J.W. Garcia; H. Sarmento; F.P. Miranda; F. Henrique-Silva
05/31/2017
Adult; Brazil; Case-control studies; Female; Hepatitis B, Chronic; HLA-DP beta-Chains; HLA-DQ Antigens; Humans; male; Middle Aged; Polymorphism, Single Nucleotide

Hepatitis B virus (HBV) infection is a serious public health problem worldwide. The progression of the disease depends on several host and viral factors and may result in fulminant hepatitis (very rare), acute hepatitis with spontaneous clearance, and chronic hepatitis B infection. Previous studies demonstrated that variations in the human leukocyte antigen (HLA) class II (HLA-DPB1 and HLA- ... more

V.R.Z.B. Pereira; J.M. Wolf; G.Z. Stumm; T.R. Boeira; J. Galvan; D. Simon; V.R. Lunge
06/20/2017
Brazil; DNA, Ribosomal; Food-Processing Industry; Fruit; Genetic variation; Hydrolysis; Pectins; Vitis; Yeasts

The genetic diversity of epiphytic yeasts from grape carposphere is susceptible to environmental variations that determine the predominant carposphere microbiota. Understanding the diversity of yeasts that inhabit grape carposphere in different environments and their pectinolytic activity is a way to understand the biotechnological potential that surrounds us and help improve winemaking. ... more

C. Filho; M.B.D. Bertéli; J.S. Valle; L.D. Paccola-Meirelles; G.A. Linde; F.G. Barcellos; N.B. Colauto
08/17/2017
Brazil; Ecosystem; Genetic variation; Manihot; Quantitative Trait, Heritable

Genetic variability of cassava (Manihot esculenta Crantz) in Brazil is wide, being this the result of natural and cultural selection during pre- and post-domestication of the species in different environments. Given the number of species of the genus found in the region (38 of a total of 98 species), the central region of Brazil was defined as the primary center of cassava diversity. Therefore ... more

B.W. Zago; M.A.A. Barelli; E.S.S. Hoogerheide; C.L. Corrêa; G.I.S. Delforno; C.J. da Silva
08/31/2017
Apolipoproteins B; Brazil; Genetic testing; Humans; Hyperlipoproteinemia Type II; Introns; Mutation, Missense; Proprotein Convertase 9; Receptors, LDL

Familial hypercholesterolemia (FH) is a dominant, autosomal disease characterized by high LDL levels in blood plasma, and is caused by a defect in the gene encoding the LDL receptor (LDLR). The clinical diagnosis is based on personal and familial history, physical examination findings, and measures of high LDL cholesterol concentrations. LDLR is a cell-surface glycoprotein that controls the ... more

G.A. Molfetta; D.L. Zanette; J.E. Santos; W.A. Silva
09/21/2017
Animals; Brazil; Dogs; Feces; Genome, Viral; Parvoviridae Infections; Parvovirus, Canine; Phylogeny; Polymorphism, Genetic; Recombination, Genetic

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
09/21/2017
Brazil; Flour; Food Industry; Glutens; Plant breeding; Protein Subunits; Triticum

Industrial wheat quality flour is related to gluten amount in grain. This study aimed to evaluate the relationship between high molecular weight (HMW) glutenins obtained by SDS-PAGE and gluten strength (W) obtained by the alveograph test and cropping environmental effect on wheat flour quality for Brazilian industry. Fifty-one cultivars/breeding lines were evaluated in three environments. The ... more

P.I. Chapla; E.S.N. Vieira; F.A. Franco; G.A. Linde; G.J. Silva; N.B. Colauto; V.S. Marchioro; I. Schuster
09/21/2017
Brazil; Chromosomes, Plant; Flowers; Hybridization, Genetic; Orchidaceae; Pigmentation; Ploidies; Polymorphism, Genetic

Studies addressing chromosome variations have elucidated many points regarding the taxonomy of the Orchidaceae. Epidendrum L. besides being one the largest orchid genera, present remarkable morphological, and inter- and intraspecific chromosome variations. Thus, based on a previous report on flower color variation in individuals of E. ibaguense (magenta, pink, white, and red), our aim was to ... more

S.R. Nóbrega; A.L.F. Coelho; C.F. Verola; I.R. Costa; R. Vilaça; F.J.F. Luz; W.F. Araújo

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