Research Article

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12/13/2013
Aquaporins; Cryopreservation; Cryoprotective agents; Intracellular ice formation; Preantral follicles

The discovery of water channels (aquaporins, AQPs) was a landmark event for the clarification of water transport through the plasma membrane. AQPs belong to a family of intrinsic membrane proteins that act as selective channels for water and for solutes such as glycerol and urea. AQPs were found in different tissues and organs, including male and female reproductive systems. In the swine ... more

A.D. Sales; C.H. Lobo; A.A. Carvalho; A.A. Moura; A.P.R. Rodrigues
07/28/2015
Chromatin accessibility; Epigenetic mechanisms; GC-rich DNA; Real-time polymerase chain reaction

In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing the fluorescent dye SYBR Green. ... more

G.J. Chang; H.M. Seyfert; X.Z. Shen
05/09/2016
2-Deoxy-2-[18F]-fluoro-D-glucose; Diffuse large B-cell lymphoma; Glucose transporters; Ki-67; Natural killer T-cell lymphoma; Real-time polymerase chain reaction

The purpose of this study was to investigate the association between cellular 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) uptake and the expression of several subtypes of glucose transporters (GLUT) and Ki-67 in diffuse large B-cell lymphoma (DLBCL) and natural killer (NK)/T-cell lymphoma (NKTCL). Cell lines were histologically determined to be DLBCL (Raji cells) and NKTCL ( ... more

Y.M. Liu; X.M. Zhai; Y.W. Wu; Y.M. Liu; X.M. Zhai; Y.W. Wu
12/02/2016
14-3-3 Proteins; Chromosome Mapping; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Multigene Family; Organ Specificity; Panicum; Phylogeny; Stress, Physiological

Members of the 14-3-3 family of proteins are conserved regulatory proteins that are widely found in eukaryotes and play crucial roles in diverse physiological processes, including responses to different stresses. Although genome-wide analysis of 14-3-3 proteins has been performed in a few plant species, it has not been performed in switchgrass. In this study, we identified 21 switchgrass 14-3- ... more

S. Wu; H.D. Yan; A.L. Zhang; L.K. Huang; G.H. Yin; S. Lee
12/02/2016
cluster analysis; Genes, Plant; Manihot; Phylogeny; Polymorphism, Single Nucleotide; Sequence alignment; Sequence Analysis, DNA; Starch

The distribution and frequency of single nucleotide polymorphisms (SNPs) can help to understand changes associated with characteristics of interest. We aimed to evaluate nucleotide diversity in six genes involved in starch biosynthesis in cassava using a panel of 96 unrelated accessions. The genes were sequenced, aligned, and used to obtain values for nucleotide diversity (π), segregating ... more

L.M. Vasconcelos; A.C. Brito; C.D. Carmo; E.J. Oliveira
12/23/2016
Animals; Cloning, Molecular; Disease resistance; Flavonoids; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Insecta; Metabolic Networks and Pathways; Pinus; Plant Diseases

Pinus massoniana Lamb. is an important timber and turpentine-producing tree species in China. Dendrolimus punctatus and Dasychira axutha are leaf-eating pests that have harmful effects on P. massoniana production. Few studies have focused on the molecular mechanisms underlying pest resistance in P. massoniana. Based on sequencing analysis of the transcriptomes of insect-resistant P. massoniana ... more

Z.Q. Yang; H. Chen; J.H. Tan; H.L. Xu; J. Jia; Y.H. Feng
1/23/2017
Gene expression; Genes, Plant; Genetic Vectors; Ginsenosides; Panax; Plants, Genetically Modified; Transformation, Genetic

Panax japonicus C.A. Meyer, a perennial herb belonging to the Araliaceae ginseng genus, is one of the seven rare and endangered Chinese medical herbs. By cloning the SS segment, the expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and introduced into Agrobactria LBA4404, which is used for engineering bacteria. Polymerase chain reaction (PCR) showed that the PjSS, antiPjSS, and ... more

L. Zhang; T.T. Wang
2/23/2017
Capsicum; Cytoplasm; Fertility; Flowers; Genes, Plant; Genetic markers; Genotype; Hybridization, Genetic; Meristem; Mutation; Phenotype; Plant breeding; Plant Infertility; Reproducibility of Results; seeds

Cytoplasmic male sterility (CMS) in pepper is a better way to produce hybrid seeds compared to manual production. We used the two sequence characterized amplified region (SCAR) markers (CRF-SCAR and CMS-SCAR) in CMS pepper, to identify the genotype. We assembled two CMS yellow bud mutants (YBM; YBM12-A and YBM12-B). This mutation in leaf color is controlled by a single dominant nuclear gene. ... more

G.S. Sun; Z.L. Dai; P.W. Bosland; Q. Wang; C.Q. Sun; Z.C. Zhang; Z.H. Ma
3/15/2017
Genes, Plant; Genetic variation; Phenotype; Plant breeding; Saccharum; Selection, Genetic; Sucrose

Sugarcane breeding programs have been adapting to a new market demand: aside from high sucrose yield per hectare, the sector needs new cultivars with higher fiber percentages. The selection of sugarcane clones based on phenotype alone is a complex task. The selected clones should display high performance in a series of yield- and quality-related traits. Selection indices can provide ... more

A.A.C. de Azeredo; L.L. Bhering; B.P. Brasileiro; C.D. Cruz; L.C.I. Silveira; R.A. Oliveira; J.C.Bespalho Filho; E. Daros
3/22/2017
Chromosomes, Plant; Colchicine; Genes, Plant; Genotype; Haploidy; Inflorescence; Plant breeding; seeds; Zea mays

Doubling method is the technical barriers in maize haploid breeding. It was very important to establish the independent intellectual property rights for doubling method. In this experiment, the maize haploid inducer, TG15, was used for producing maternal haploids. Also, haploids were obtained from two kinds of maternal genotypes involved in the experiment, including high-oil type and common ... more

L. Jiang; X.Y. Yang; X.Y. Li; P.K. Deng; G.X. Jing; X.Q. Wang; Z. Xing; R.G. Zhao

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