Research Article

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The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potential inoculant to the crop. We evaluated the genetic diversity of PGPB ... more

J.E.L. Antunes; M.C.C.P. Lyra; F.J. Ollero; A.D.S. Freitas; L.M.S. Oliveira; A.S.F. Araújo; M.V.B. Figueiredo
05/18/2017
Archaea; Bacteria; Brazil; Lakes; Microbiota; Rivers; RNA, Ribosomal, 16S

The Amazon region has the largest hydrographic basin on the planet and 
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Solimões River, one of its main water courses. To investigate the 
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D. Toyama; C.D. Santos-Júnior; L.T. Kishi; T.C.S. Oliveira; J.W. Garcia; H. Sarmento; F.P. Miranda; F. Henrique-Silva
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Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

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4/05/2017
Animals; Brazil; Cattle; Cattle Diseases; Coronavirus Infections; Coronavirus, Bovine; Dysentery; Evolution, Molecular; Feces; Membrane Glycoproteins; Phylogeny; Sequence Analysis, DNA; Viral Envelope Proteins; Viral Tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of respiratory disease in ... more

E.A. Beuttemmuller; A.F. Alfieri; S.A. Headley; A.A. Alfieri
08/17/2017
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Diarrhea is considered the second most common cause of infant mortality worldwide. The disease can be caused by many different pathogens, including diarrheagenic Escherichia coli (DEC), which includes the pathotypes enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and enteropathogenic E. coli (EPEC). To ... more

J.W. Vendruscolo; T.L. Waldrich; G.I.A. Saikawa; J.S. Pelayo; R.K.T. Kobayashi; G. Nakazato; S.P.D. Rocha
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With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
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Members of the Eimeria genus are protozoan parasites of the subphylum Apicomplexa (Eimeriidae family), and belong to the coccidia group. Eimeria tenella is one of the most pathogenic species owing to its ability to penetrate the mucosa, and cause inflammation and damage. It is an obligate intracellular parasite that causes disease by destroying the host cells during multiplication. Heat shock ... more

A.L.G. Bogado; G.F. Martins; J.P. Sasse; daS. Guimarães; J.L. Garcia
2/08/2017
Animals; Genome, Mitochondrial; Hedgehogs; Open Reading Frames; Phylogeny; RNA, Ribosomal; RNA, Ribosomal, 16S; RNA, Transfer

We sequenced and characterized the complete mitogenome (KX964606) of the Amur hedgehog Erinaceus amurensis to provide more data for comparative mitogenomics of the genus Erinaceus (Erinaceidae). The mitogenome of E. amurensis is a circular molecule 16,941 bp long, consisting of a control region and a conserved set of 37 genes containing 13 protein-coding genes, 22 tRNA genes, and two rRNA ... more

N.H. Kim; S.J. Lim; H.M. Chae; Y.C. Park
3/08/2017
Animals; Bacteria; Biodiversity; Cheese; China; Cultured Milk Products; DNA, Bacterial; Food Microbiology; Fungi; Milk; RNA, Ribosomal, 16S; RNA, Ribosomal, 18S; Sequence Analysis, DNA

Hurood cheese (HC) and Jueke (Jk) are 2 traditional fermented dairy products produced from raw milk (RM) in the Inner Mongolia region of China. They have a long history of production and consumption. The microbial compositions of RM, HC, and Jk vary greatly, and are influenced by their geographical origins and unique processing methods. In this study, 2 batches of RM, HC, and Jk samples were ... more

M.L. Gao; H.M. Hou; X.X. Teng; Y.L. Zhu; H.S. Hao; G.L. Zhang
11/01/2011
Cellulose-degrading activity; Microbiota; Sphenophorus levis; Sugarcane weevil

The sugarcane weevil, Sphenophorus levis, is a wide-spread sugarcane pest in Brazil. Sphenophorus levis may depend on microorganisms that inhabit its intestinal tract. We examined the diversity of the gut microbiota of S. levis, which was characterized using culture-dependent and culture-independent methods. Analysis of 16S rRNA amplified directly from the gut ... more

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