Research Article

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Anticarcinogenic Agents; Apoptosis; bcl-2-Associated X Protein; Breast neoplasms; Carotenoids; Cell proliferation; Humans; Lycopene; MCF-7 cells; Tumor Suppressor Protein p53

Breast cancer adversely affects the health status of women; therefore, the prevention and treatment of breast cancer is of critical importance. Lycopene is known to possess several biological effects such as removal of free radicals, alleviation of biological oxidative injury, and inhibition of tumor growth. In this study, we aimed to illustrate the effect of lycopene on tumor cell ... more

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Animals; Gene frequency; Heterozygote; Humans; Polymorphism, Genetic; Software

The gene diversity or expected heterozygosity (H) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of H can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a ... more

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Antibiotics, Antineoplastic; Apoptosis; Bleomycin; Cell line; Endoplasmic Reticulum Chaperone BiP; Endoplasmic reticulum stress; Heat-shock proteins; Human Umbilical Vein Endothelial Cells; Humans; Receptors, G-Protein-Coupled; Transcription Factor CHOP

In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK- ... more

Y.D. Huang; X. Tong; Q. Chen; S.W. Xia; Y. Zhuo; Z.H. Kang; X.Y. Xue
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Cell line; Cell proliferation; Epidermal growth factor; Humans; Ki-67 Antigen; Mesangial cells; Vitamin D; Vitamins

Previous studies have found that 1,25-dihydroxyvitamin D [1,25(OH)D or VD] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)D on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and ... more

D. Zhao; C.J. Zhang; R. Yang; J.P. Chen; L. Ma; G. Liu; X.P. Yang
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Cell Line, Tumor; Drugs, Chinese Herbal; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Keratinocytes; Panax; Scutellaria; Ultraviolet Rays

Hypoxia-inducible factor-1α (HIF-1α) is considered the main transcriptional regulator of the hypoxia-specific cellular and developmental response. This study was performed to investigate the effect of Shenqin biochemical extract (SQBE) on HIF-1α expression in ultraviolet B (UVB)-irradiated HaCaT cells and the possible action mechanisms of SQBE against UVB-induced skin cancer. HaCaT cells in ... more

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Antineoplastic Agents; Apoptosis; Cell Line, Tumor; Cell proliferation; Colorectal neoplasms; Curcumin; Drug Resistance, Neoplasm; Heat-shock proteins; HSP27 Heat-Shock Proteins; Humans; Inhibitor of Apoptosis Proteins; Molecular chaperones; Proto-Oncogene Proteins c-bcl-2; Survivin

Since there are no studies on the reversal of multidrug resistance by curcumin in the human colorectal cancer cell line HCT-8/5-FU, our aim was to search for highly efficient reversal agents and investigate the underlying mechanisms of this reversal. The cytotoxic effects of curcumin and 5-FU on HCT-8 and HCT-8/5-FU cells and the reversal effects of 5-FU in combination with curcumin on HCT-8/5 ... more

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Asian People; DNA, Mitochondrial; Haplotypes; Human Migration; Humans; Polymorphism, Genetic; Thailand

The lower northern part of Thailand contains various genetically diverse ethnic populations. The sequences of the mitochondrial DNA hypervariable region were studied in three ethnic populations inhabiting Phitsanulok Province. One hundred and nine nucleotide sequences - 53, 29, and 27 from Hmongs (Hill tribe), Lao Songs, and Thai-Siams, respectively - were collected. The haplotypes were ... more

U. Suyasunanont; M. Nakkuntod; S. Mirasena
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Cells, Cultured; DNA damage; Euterpe; Fruit; Hep G2 Cells; Humans; Lymphocytes; Plant Oils

Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and ... more

E.S. Marques; M.S.F. Tsuboy; J.C.T. Carvalho; P.C.P. Rosa; F.F. Perazzo; I.O.M. Gaivão; E.L. Maistro
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Animals; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Feces; Humans; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Sensitivity and Specificity; Water Microbiology

Diarrhea is considered the second most common cause of infant mortality worldwide. The disease can be caused by many different pathogens, including diarrheagenic Escherichia coli (DEC), which includes the pathotypes enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and enteropathogenic E. coli (EPEC). To ... more

J.W. Vendruscolo; T.L. Waldrich; G.I.A. Saikawa; J.S. Pelayo; R.K.T. Kobayashi; G. Nakazato; S.P.D. Rocha
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Cell Line, Tumor; Cell Movement; Cell proliferation; Down-regulation; Glioma; Humans; MicroRNAs

Neuroglioma is associated with high rates of malignancy, metastasis, and recurrence. Recently, research on the roles of microRNAs (miR) in cancer prognosis has formed an important area of research as differential expression of miRNAs has been observed in different cancers. However, the detailed mechanism by which miRNAs regulate glioma remains unknown. Thus, we investigated the effect of miR-1 ... more

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