Research Article

Related GMR Articles

05/04/2017
Alleles; Animals; Cyprinidae; Expressed sequence tags; Gene Transfer, Horizontal; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic

We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuciscus baicalensis populations in Xinjiang. There were 4-27 (average ... more

D.J. Lei; G. Zhao; P. Xie; Y. Li; H. Yuan; M. Zou; J.G. Niu; X.F. Ma
03/08/2017
Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
02/08/2017
Alleles; Animals; Bivalvia; Evolution, Molecular; Genetics, Population; Heterozygote; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic

Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp and the number of alleles per locus varied between 2 and 23. ... more

Y.W. Feng; H.L. Jiang; Y.T. Liu; X.P. Wu; X.Q. Liu; X.M. Wei
03/22/2017
Alleles; Brazil; Fruit; Genetic markers; Genetic variation; Microsatellite Repeats; Myrtaceae; Phylogeny; Plant breeding; Polymorphism, Genetic; Trees; Vitamin A

Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encouraged fruit farmers to produce it. In order to identify and select ... more

C.F. Nunes; T.A. Setotaw; M. Pasqual; E.A. Chagas; E.G. Santos; D.N. Santos; C.G.B. Lima; G.M.A. Cançado
03/30/2017
Alleles; Crops, Agricultural; Gene frequency; Genetic markers; Genetic variation; Genotype; Microsatellite Repeats; Phylogeny; Plant breeding; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; Zea mays

Genetic diversity in crops is essential to make improvements related to superior germplasms. Implementation of molecular markers to identify suitable genotypes speeds up the breeding progress by enhancing selection efficiency. This study was carried out to probe genetic diversity among 21 maize genotypes using 20 inter simple sequence repeat (ISSR) markers. We identified a total of 190 ... more

R.W. Muhammad; A. Qayyum; M.Q. Ahmad; A. Hamza; M. Yousaf; B. Ahmad; M. Younas; W. Malik; S. Liaqat; E. Noor
05/10/2017
Alleles; Anacardiaceae; Genetic markers; Microsatellite Repeats; Plant breeding; Polymorphism, Genetic; Trees

Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associated DNA sequencing (RAD-seq) method and examined their ... more

Z.F. Wang; H.L. Cao; L.F. Wu; Y. Guo; Q.M. Mei; M. Li; Y. Wang; Z.M. Wang
06/29/2017
Arecaceae; DNA Primers; Endangered species; Genetic markers; Heterozygote; Microsatellite Repeats; Polymerase chain reaction; Polymorphism, Genetic

The licuri palm Syagrus coronata plays a key role in the ecology and economy of Brazilian semiarid region. Nonetheless, genetic data about populations of this species are absent even though the intensive and uncontrolled exploitation since colonial periods has threatened the sustainability and viability of licuri populations. Therefore, we attempted to test the efficacy of transferability of ... more

R.R. Simplicio; D.G. Pereira; A.M. Waldschmidt
08/17/2017
Animals; Expressed sequence tags; Malaysia; Microsatellite Repeats; Palaemonidae; Polymorphism, Genetic; Selective breeding

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock improvement program is now important to avoid the depletion of wild ... more

K.H. Atin; A. Christianus; N. Fatin; A.C. Lutas; M. Shabanimofrad; B. Subha
08/17/2017
Animals; Gene Amplification; Gene flow; Gene Transfer, Horizontal; Linkage disequilibrium; Microsatellite Repeats; Perciformes; Polymorphism, Genetic

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 ... more

F.F. Chen; C.Y. Ma; L.P. Yan; H. Zhang; W. Wang; Y. Zhang; L.B. Ma
03/30/2017
Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo

Pages