Research Article

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Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

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Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
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Alleles; Animals; Bivalvia; Evolution, Molecular; Genetics, Population; Heterozygote; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic

Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp and the number of alleles per locus varied between 2 and 23. ... more

Y.W. Feng; H.L. Jiang; Y.T. Liu; X.P. Wu; X.Q. Liu; X.M. Wei
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Animals; Expressed sequence tags; Malaysia; Microsatellite Repeats; Palaemonidae; Polymorphism, Genetic; Selective breeding

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock improvement program is now important to avoid the depletion of wild ... more

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Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

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Alleles; Cucurbitaceae; Genotype; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic

Taking into account the scenario of melon production (Cucumis melo L.) in Brazil, it is notable the importance of the culture in the fruit market. The preference of the national market is concentrated in the fruits of the yellow type. Therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes in the breeding process, and ... more

N. Carvalho; F.M. Canela; P.H.S. Leite; M.A. Ferreira; V.R. Oliveira; M.F. Santos; N.O.S. Souza; G.S.C. Buso
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Animals; Gene Amplification; Gene flow; Gene Transfer, Horizontal; Linkage disequilibrium; Microsatellite Repeats; Perciformes; Polymorphism, Genetic

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 ... more

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Alleles; Brazil; Fruit; Genetic markers; Genetic variation; Microsatellite Repeats; Myrtaceae; Phylogeny; Plant breeding; Polymorphism, Genetic; Trees; Vitamin A

Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encouraged fruit farmers to produce it. In order to identify and select ... more

C.F. Nunes; T.A. Setotaw; M. Pasqual; E.A. Chagas; E.G. Santos; D.N. Santos; C.G.B. Lima; G.M.A. Cançado
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Genetic diversity in crops is essential to make improvements related to superior germplasms. Implementation of molecular markers to identify suitable genotypes speeds up the breeding progress by enhancing selection efficiency. This study was carried out to probe genetic diversity among 21 maize genotypes using 20 inter simple sequence repeat (ISSR) markers. We identified a total of 190 ... more

R.W. Muhammad; A. Qayyum; M.Q. Ahmad; A. Hamza; M. Yousaf; B. Ahmad; M. Younas; W. Malik; S. Liaqat; E. Noor
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Alleles; Amelogenin; Asian Continental Ancestry Group; China; Chromosomes, Human, X; Chromosomes, Human, Y; DNA fingerprinting; Ethnic groups; Female; Forensic genetics; Gene frequency; Genetics, Population; Haplotypes; Humans; male; Microsatellite Repeats; Paternity; Polymorphism, Genetic

The sex-linked short tandem repeats (STR), Y-STR and X-STR, are important for autosomal STRs in forensic paternity testing. We evaluated the forensic parameters of 19 Y-STRs and 16 X-STRs in the Han population of Shandong province, China. A Goldeneye 20Y kit (DYS391, DYS389I, DYS390, DYS389II, DYS348, DYS456, Y-GATA-H4, DYS447, DYS19, DYS392, DYS393, DYS388, DYS439, DYS635, DYS448, DYS460, ... more

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