Research Article

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Gene expression; Lignin; Quantitative real-time PCR; seeds; Soybean

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied ... more

A. Baldoni; E.V.R. Von Pinho; J.S. Fernandes; V.M. Abreu; M.L.M. Carvalho
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Coenzyme specificity; Malate dehydrogenase; Site-directed mutagenesis; Streptomyces coelicolor

We describe here for the first time the alteration of coenzyme specificity of malate dehydrogenase (MDH) from Streptomyces coelicolor A3(2) (ScMDH). In the present study, we replaced four amino acid residues in the Rossmann fold (βB-αC) region of NADH-dependent ScMDH by site-directed mutagenesis with those of NADPH-dependent MDH (Glu42Gly, Ile43Ser, Pro45Arg, ... more

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08/19/2015
Genetic diversity; Random amplification of polymorphic DNA; Zea mays

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To ... more

F.S. Resh; C.A. Scapim; C.A. Mangolin; M.F.P.S. Machado; A.Tdo Amaral; H.C.C. Ramos; M. Vivas
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EST-SSR markers; Genetic diversity; Reciprocal recurrent selection; Zea mays

This study aimed to improve grain yield in the full-sib reciprocal recurrent selection program of maize from the North Flumi­nense State University. In the current phase of the program, the goal is to maintain, or even increase, the genetic variability within and among populations, in order to increase heterosis of the 13th cycle of reciprocal recurrent selection. Microsatellite ... more

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Cytokinin/auxin; Endogenous hormones; Opposite phyllotaxy ratio; Phyllotaxy; Temperature; Zea mays

Newly identified maize (Zea mays) mutants with opposite leaf phyllotaxy are important in the study of the maize crop. Previous studies have revealed the developmental mechanism of opposite phyllotaxy on the physiological, cellular, and molecular levels. However, there have been few reports regarding the effects of changes in endogenous hormone levels in maize leaf primordia under ... more

H. Ye; G.M. Han; Q. Ma; Y.Q. Tan; H.Y. Jiang; S.W. Zhu; B.J. Cheng
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cDNA-AFLP; Heterosis; Non-additive gene expression; Transcriptome level; Zea mays

Heterosis has greatly contributed to conventional plant breeding and is widely used to increase crop plant productivity. However, although some studies have explored the mechanisms of heterosis at the genomic and transcriptome level, these mechanisms still remain unclear. The growth and development of maize seedlings and immature embryos have an important impact on subsequent production. ... more

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Grain yield; Multi-trait selection; Popping expansion; Zea mays

One of the major difficulties faced by popcorn breeders is the negative correlation between popping expansion (PE) and grain yield (GY). It is necessary to overcome this difficulty to obtain promising genotypes. One helpful tool in this process is a selection index because it allows multiple features of interest to be selected. Thus, the present study proposes a new and comprehensive ... more

R.A. Vieira; R. Rocha; C.A. Scapim; A.T.Amaral Júnior; M. Vivas; R.A. Vieira; R. Rocha; C.A. Scapim; A.T.Amaral Júnior; M. Vivas; R.A. Vieira; R. Rocha; C.A. Scapim; A.T.Amaral Júnior; M. Vivas
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Agronomic traits; Heterosis; Zea mays

Currently, one of the acclaimed alternatives for increasing maize yield is accomplished through utilization of heterosis in hybrid cultivars. However, upon performing crosses between related pairs, there are losses due to inbreeding depression. Thus, the aim of this study was to estimate inbreeding depression and the contribution of loci in heterozygosity in different types of maize ... more

F.B.S. Botelho; A.T. Bruzi; I.P. Lima; C.S. Rodrigues; R.Tde C. Botelho; F.B.S. Botelho; A.T. Bruzi; I.P. Lima; C.S. Rodrigues; R.Tde C. Botelho
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Heat shock; Late embryogenesis; Protein abundance; Zea mays

Low water availability is one of the factors that limit agricultural crop development, and hence the development of genotypes with increased water stress tolerance is a challenge in plant breeding programs. Heat-resistant proteins have been widely studied, and are reported to participate in various developmental processes and to accumulate in response to stress. This study aimed to ... more

V.M. Abreu; I.C.Silva Neta; E.V.R. Von Pinho; G.M.F. Naves; R.M. Guimarães; H.O. Santos; R.G. Von Pinho; V.M. Abreu; I.C.Silva Neta; E.V.R. Von Pinho; G.M.F. Naves; R.M. Guimarães; H.O. Santos; R.G. Von Pinho
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Crosses, Genetic; Genotype; Phenotype; Quantitative trait loci; Zea mays

The successful development of hybrid cultivars depends on the reliability of estimated combining ability of the parent lines. The objectives of this study were to assess the combining ability of partially inbred S families of popcorn derived from the open-pollinated variety UENF 14, via top-crosses with four testers, and to compare the testers for their ability to discriminate the S progenies ... more

V.J. de Lima; A.T. do Amara Junior; S.H. Kamphorst; G.F. Pena; J.T. Leite; K.F.M. Schmitt; C. Vittorazzi; J.E. de Almei Filho; F. Mora

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