Research Article

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2017 Jun 29
Animals; CpG islands; DNA methylation; fucosyltransferases; Galactoside 2-alpha-L-fucosyltransferase; Glycosphingolipids; Promoter Regions, Genetic; Swine; Transcriptional activation

Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq results using bioinformatics tools. The FUT1 transcription initiation ... more

W.Y. Qin; L.N. Gan; R.W. Xia; W.H. Dong; S.Y. Sun; G.Q. Zhu; S.L. Wu; W.B. Bao
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Adult; Animals; Case-control studies; Dermatophagoides pteronyssinus; Female; Humans; Interleukin-18; male; Middle Aged; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Rhinitis, Allergic, Perennial; Thailand

Allergic rhinitis (AR) is an IgE-mediated inflammation of the nasal membranes, which is naturally triggered by aeroallergens. House dust mites (HDM) are the most common inhalant allergens. Interleukin-18 (IL-18) has been established as an essential cytokine that can activate the generation of IgE. This randomized controlled study aimed to identify the possible relationship of the genetic ... more

A. Tungtrongchitr; J. Jumpasri; N. Sookrung; N. Visitsunthorn; P. Tantilipikorn; O. Piboonpocanan; N. Indrawattana; R. Tungtrongchitr; W. Chaicumpa
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Animals; Expressed sequence tags; Malaysia; Microsatellite Repeats; Palaemonidae; Polymorphism, Genetic; Selective breeding

The Malaysian giant prawn is among the most commonly cultured species of the genus Macrobrachium. Stocks of giant prawns from four rivers in Peninsular Malaysia have been used for aquaculture over the past 25 years, which has led to repeated harvesting, restocking, and transplantation between rivers. Consequently, a stock improvement program is now important to avoid the depletion of wild ... more

K.H. Atin; A. Christianus; N. Fatin; A.C. Lutas; M. Shabanimofrad; B. Subha
2017 Aug 17
Animals; Diptera; DNA, Mitochondrial; Electron Transport Complex IV; Glycine max; Insect Proteins; Paraguay

We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Agromyzidae, was also noted in soybean fields from the Canindeyú, Alto ... more

J.V.C. Guedes; J.A. Arnemann; L.E. Curioletti; L.M. Burtet; M.L. Ramírez-Paredes; D. Noschang; I. de Oliveira; W.T. Tay
08/17/2017
Animals; Gene Amplification; Gene flow; Gene Transfer, Horizontal; Linkage disequilibrium; Microsatellite Repeats; Perciformes; Polymorphism, Genetic

In this study, 10 polymorphic microsatellite markers were developed in Scomber japonicus and were examined on 30 individuals collected from the North Pacific. The number of alleles per locus ranged from 4 to 17. The observed and expected heterozygosities per locus ranged from 0.2759 to 0.8621 and from 0.43071 to 0.9177, respectively. The polymorphism information content (PIC) was from 0.3931 ... more

F.F. Chen; C.Y. Ma; L.P. Yan; H. Zhang; W. Wang; Y. Zhang; L.B. Ma
08/17/2017
Animals; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Feces; Humans; Molecular Diagnostic Techniques; Multiplex polymerase chain reaction; Sensitivity and Specificity; Water Microbiology

Diarrhea is considered the second most common cause of infant mortality worldwide. The disease can be caused by many different pathogens, including diarrheagenic Escherichia coli (DEC), which includes the pathotypes enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and enteropathogenic E. coli (EPEC). To ... more

J.W. Vendruscolo; T.L. Waldrich; G.I.A. Saikawa; J.S. Pelayo; R.K.T. Kobayashi; G. Nakazato; S.P.D. Rocha
08/31/2017
Alveolar Epithelial Cells; Animals; CD11b Antigen; Female; male; Mice; Mice, Inbred C57BL; Pulmonary Surfactant-Associated Proteins; Respiratory Insufficiency

As a β2 integrin family member, Mac-1 plays an important role in the inflammatory response. Inflammation and lung injury are closely associated, but the involvement of Mac-1 in the occurrence and development of such pathologies remains poorly understood. We aimed to investigate the relationship between Mac-1 deficiency and respiratory failure in Mac-1 knockout {Mac-1} mice, using C57BL/6J mice ... more

J. Wang; Y.B. Ci; C.L. Liu; H.M. Sun
08/31/2017
Animals; DNA Barcoding, Taxonomic; DNA, Ribosomal Spacer; Genome, Insect; Hymenoptera; Lepidoptera; Microsatellite Repeats

In Brazil, the species Diatraea flavipennella and D. saccharalis play an important role in the sugar and alcohol agribusiness by causing many damages in sugarcane fields. The egg, larva, pupa, and adult stages are very morphologically similar between these species, and the identification can be confused. The internal transcribed spacer 2 (ITS 2) from ribosomal DNA has important features as ... more

M.S.Regueira Neto; R.P. Barros; M.A.J. Morais; V.Q. Balbino; V. Loreto
09/21/2017
Animals; Evolution, Molecular; Hybridization, Genetic; Lepidoptera; Polymorphism, Genetic

Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and tomatoes. The wide range of crops exploited by H. armigera (mainly ... more

H.M. Lopes; C.S. Bastos; L.S. Boiteux; J. Foresti; F.A. Suinaga
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Animals; Blastocyst; Carps; Fish Proteins; Fructose-Bisphosphate Aldolase; Gene Expression Regulation, Developmental; Organ Specificity

Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model owing to its relatively high ... more

J.J. Fan; J.J. Bai; D.M. Ma; L.Y. Yu; P. Jiang

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