Research Article

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Case-control design; Childhood asthma; Chinese population; Genetic association studies; ORM1-like 3 (ORMDL3) gene; Single nucleotide polymorphisms

Single nucleotide polymorphism (SNP)-based genome-wide association studies have revealed that polymorphisms of the ORM1-like 3 (ORMDL3) gene are associated with childhood asthma. We investigated genetic associations of SNPs in and around the ORMDL3 gene with childhood asthma in a Chinese population. Genomic DNA was extracted from peripheral venous blood drawn from 152 subjects with ... more

F.F. Yang; Y. Huang; Q.B. Li; J.H. Dai; Z. Fu
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Alleles; HLA-DRB1; Neighbor-joining dendrograms; Principal component analysis

We established a genetic database by investigating human leukocyte antigen (HLA)-DRB1 allelic frequencies in a disease-association study in the Tujia population in Wufang, Hubei, China. The allele frequencies of the HLA-DRB1 locus in 262 healthy, unrelated Tujia individuals living in the Wufeng region of the Hubei Province were analyzed using the Luminex HLA sequence-specific ... more

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Because of their local adaptation and economic factors that limit the adoption of commercial hybrids, farmer-saved maize landraces are still grown over a considerable area concentrated in southwest China. To evaluate the potential of using maize landraces, the germplasm characteristics of 96 landraces from southwest China were evaluated at phenotypic, cellular, and molecular levels. The ... more

F.B. Chen; Q.L. Yao; H.F. Liu; P. Fang
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Alleles; Animals; Bivalvia; Evolution, Molecular; Genetics, Population; Heterozygote; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic

Solen grandis is an important economic and overexploited bivalve species. In order to perform its fine-scale genetic analyses, 105 pairs of microsatellites with polymorphism were identified through Illumina Hiseq platform and bioinformatic assembly technology in this study. The estimated fragment size ranged from 100 to 268 bp and the number of alleles per locus varied between 2 and 23. ... more

Y.W. Feng; H.L. Jiang; Y.T. Liu; X.P. Wu; X.Q. Liu; X.M. Wei
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Alleles; DNA, Plant; Expressed sequence tags; Genetic variation; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic; Reproduction; Saccharum

The reduction in sugarcane productivity in subsequent cutting stages may be related to a gradual decrease of the allele number and mean observed heterozygosity (H) in the sugarcane ratoon. This hypothesis was tested assessing the number of alleles and H values in 10 expressed sequence tag microsatellites (Est-SSR loci) of the sugarcane varieties RB72454 and RB867515 in different cutting stages ... more

R. Augusto; R.C. Maranho; C.A. Mangolin; J.C.Bespalho Filho; M.F.P.S. Machado
3/22/2017
Alleles; Biofuels; Crosses, Genetic; Genotype; Jatropha; Multivariate analysis; Phenotype; Plant breeding; Plant Oils

Jatropha is research target worldwide aimed at large-scale oil production for biodiesel and bio-kerosene. Its production potential is among 1200 and 1500 kg/ha of oil after the 4th year. This study aimed to estimate combining ability of Jatropha genotypes by multivariate diallel analysis to select parents and crosses that allow gains in important agronomic traits. We performed crosses in ... more

P.E. Teodoro; E.V. Rodrigues; L.A. Peixoto; L.A. Silva; B.G. Laviola; L.L. Bhering
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Alleles; Biomass; Genetic variation; Introduced species; Microsatellite Repeats; Sapium; Trees; United States

Chinese tallow (Triadica sebifera) is an important crop and ornamental tree. After it was introduced into the USA, it gradually became a noxious invasive tree in south-eastern America since the middle of the 1900s. Because only six microsatellites were reported previously in T. sebifera, to better understand the genetic diversity and population dynamics of such species, we reported here 28 new ... more

Y.F. Zhuang; Z.F. Wang; L.F. Wu
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Alleles; Animals; Cyprinidae; Expressed sequence tags; Gene Transfer, Horizontal; Heterozygote; Microsatellite Repeats; Polymorphism, Genetic

We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuciscus baicalensis populations in Xinjiang. There were 4-27 (average ... more

D.J. Lei; G. Zhao; P. Xie; Y. Li; H. Yuan; M. Zou; J.G. Niu; X.F. Ma
05/10/2017
Alleles; Animals; Expressed sequence tags; Genetic markers; Heterozygote; Microsatellite Repeats; Perciformes; Polymorphism, Genetic; Transcriptome

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

P.P. Wang; M. Ren; S.Q. Chen; S.W. Yin; C. Zhao; H.Y. Zhang; X.R. Li; Q.Q. Cao; G.Q. Zhou
05/10/2017
Alleles; Anacardiaceae; Genetic markers; Microsatellite Repeats; Plant breeding; Polymorphism, Genetic; Trees

Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associated DNA sequencing (RAD-seq) method and examined their ... more

Z.F. Wang; H.L. Cao; L.F. Wu; Y. Guo; Q.M. Mei; M. Li; Y. Wang; Z.M. Wang

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