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Adipose-derived stem cells (ADSCs) show nearly unlimited potential in medical and animal science. Currently, understanding of the biological mechanisms regulating ADSC growth in vitro remains very limited. Histone acetylation, an epigenetic modification, plays a key role in maintaining stem cell properties. To further study its effect on ADSC growth ... more

X. Wang; F.X. Zhang; Z.M. Wang; Q. Wang; H.F. Wang; Y. Ren; D.P. Tai; H. Liang; D.J. Liu; X. Wang; F.X. Zhang; Z.M. Wang; Q. Wang; H.F. Wang; Y. Ren; D.P. Tai; H. Liang; D.J. Liu
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The neomycin-resistance (neor) gene is widely used as a selectable marker in eukaryotic expression vectors; however, its expression often affects that of target genes. Cre recombinase recognizes LoxP sites, leading to site-specific recombination and deletion of DNA and RNA between two LoxP sites. In the present study, a humanized Fat-1 gene (hFat-1) was generated by DNA Works ... more

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Colorectal neoplasms; DCC gene; Transfection

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The aim of this study was to investigate the expression of miR-21 in esophageal cancer and the impact of miR-21 on apoptosis, invasion, and the expression of target genes in esophageal cancer cells. Fluorescence quantitative polymerase chain reaction analysis was used to detect the expression of miR-21 in human esophageal tissues, adjacent tissues, and an esophageal cancer cell line (TE- ... more

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