Research Article

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05/25/2017
Cell line; Cell proliferation; Epidermal growth factor; Humans; Ki-67 Antigen; Mesangial cells; Vitamin D; Vitamins

Previous studies have found that 1,25-dihydroxyvitamin D [1,25(OH)D or VD] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)D on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and ... more

D. Zhao; C.J. Zhang; R. Yang; J.P. Chen; L. Ma; G. Liu; X.P. Yang
05/31/2017
Animals; Carbohydrates; Cell Line, Tumor; Cell proliferation; DNA damage; Food Handling; Food Quality; Food storage; Fruit and Vegetable Juices; Rats; Ultraviolet Rays; Vitamins; Vitis

Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) ... more

E. Düsman; I.V. Almeida; E.P. Pinto; L. Lucchetta; V.E.P. Vicentini
2/16/2017
Caco-2 Cells; Cell Line, Tumor; Colonic Neoplasms; Equilibrative Nucleoside Transporter 1; Equilibrative-Nucleoside Transporter 2; Gene expression; Gene Expression Regulation, Neoplastic; HCT116 Cells; Humans; Neoplasm Metastasis

Human equilibrative nucleoside transporters (hENT) 1 and 2, encoded by SLC29A1 and SLC29A2, permit the bidirectional passage of nucleoside analogues into cells and may correlate with clinical responses to chemotherapy in patients with colorectal cancer (CRC). The purpose of this study was to evaluate the expression profiles of SLC29A1 and SLC29A2 in human cancer cell lines. Using quantitative ... more

Y. Liu; T. Zuo; X. Zhu; N. Ahuja; T. Fu
05/10/2017
Aged; Biomarkers, Tumor; Carcinoma, Squamous Cell; Cell Adhesion Molecule-1; Cell Adhesion Molecules; Esophageal Neoplasms; Female; Gene Expression Regulation, Neoplastic; Humans; Immunoglobulins; male; Middle Aged; Neoplasm Metastasis; RNA, Messenger; Up-Regulation

The mRNA expression of cell adhesion molecule 1 (CADM1) and its clinicopathological significance in esophageal squamous cell carcinoma (ESCC) tissues were investigated. CADM1 mRNA and protein expression were detected in tissue samples from 50 patients with ESCC by reverse transcription-polymerase chain reaction (RT-PCR) and streptavidin-peroxidase (SP) immunohistochemistry; adjacent tissues ... more

J.B. Qian; H.B. Liu; Y. Zhu; F. Lu; Q.C. Yang; Y. Shen
12/21/2015
Apoptosis; Aquaporin; Cell proliferation; Diabetes; Myocardial cell

The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric analysis of Annexin V-fluorescein ... more

P.F. Zhao; M.J. Sun
11/23/2015
Apoptosis; Cell proliferation; Checkpoint kinase 1; Gamma-aminobutyric acid type A receptor; p53; Propofol

We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. The effect of propofol on the proliferation and viability of rENSCs was ... more

J.W. Wang; W.W. Cheng; T. Xu; Z.Y. Yang
12/19/2016
Animals; Antimutagenic Agents; Cell line; Cell proliferation; DNA damage; Dose-Response Relationship, Drug; Plant Extracts; Rats; Vanillic Acid

The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used ... more

I.V. Almeida; F.M.L. Cavalcante; V.E.P. Vicentini
1/23/2017
Adipose tissue; Animals; Cell differentiation; Cell proliferation; Estradiol; Female; Pregnancy; Progesterone; Rats; Stem cells

Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem cells (ADSCs), which are a type of adult mesenchymal stem cells, can ... more

J.N. Li; Y. Zhang; Y.F. Wang; J.Y. Chen
10/05/2015
BCL2 gene; Cell cycle; GES-1 cells; Human gastric epithelial cells; Transfection

We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vectors carrying the NeoR gene were ... more

G.Y. Shi; L.L. Zhao; K. Zhang; H.X. Zhou; A.H. Liu; J. Li; G. Li; L.H. Zhu
1/23/2017
Cell cycle; Chromosomal Instability; Chromosome Fragile Sites; Festuca; Lolium; RNA, Ribosomal

Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent ... more

L.C. Rocha; G.A. Silva; F.O. Bustamante; R.A.D. Silveira; A. Mittlemann; V.H. Techio

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