Research Article

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BCL2 gene; Cell cycle; GES-1 cells; Human gastric epithelial cells; Transfection

We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vectors carrying the NeoR gene were ... more

G.Y. Shi; L.L. Zhao; K. Zhang; H.X. Zhou; A.H. Liu; J. Li; G. Li; L.H. Zhu
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Cell cycle; Chromosomal Instability; Chromosome Fragile Sites; Festuca; Lolium; RNA, Ribosomal

Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent ... more

L.C. Rocha; G.A. Silva; F.O. Bustamante; R.A.D. Silveira; A. Mittlemann; V.H. Techio
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Brain Neoplasms; Case-control studies; China; Female; Fusion Regulatory Protein 1, Light Chains; Glioma; Humans; male; Middle Aged; Polymorphism, Single Nucleotide

Gliomas are brain tumors that can be seriously damaging to human health. The SLC7 family is involved in amino acid or peptide transportation. The relationship between SLC7A7 polymorphisms and the development of glioma has been reported previously by a few studies. Therefore, we performed a hospital based case-control study to investigate the association of three common SNPs (rs12888930, ... more

H. Zhang; W.J. Shi
02/16/2017
Alzheimer disease; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Gene Expression Regulation; Hippocampus; Humans; Maze Learning; Mice; MicroRNAs; Receptor, Cannabinoid, CB2

Alzheimer's disease (AD) is a neurodegenerative disorder, and is the most common type of dementia in the elderly population. Growing evidence indicates that microRNAs (miRNAs) play a crucial role in neuroinflammation associated with AD progression. In this study, we analyzed the expression of microRNA-139 (miR-139) as well as the learning and memory function in AD. We observed that the miR-139 ... more

Y. Tang; J.S. Bao; J.H. Su; W. Huang
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Antibiotics, Antineoplastic; Apoptosis; Bleomycin; Cell line; Endoplasmic reticulum stress; Human Umbilical Vein Endothelial Cells; Humans; Receptors, G-Protein-Coupled; Transcription Factor CHOP

In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK- ... more

Y.D. Huang; X. Tong; Q. Chen; S.W. Xia; Y. Zhuo; Z.H. Kang; X.Y. Xue
01/23/2017
Gene expression; Humans; Parkinson disease; Prion Proteins; REM Sleep Behavior Disorder; RNA, Messenger

Parkinson's disease (PD) is one of the most common neurodegenerative diseases and mainly manifests with decreasing numbers of dopaminergic neurons. Rapid eye movement (REM) sleep behavior disorder (RBD) has an incidence of 15-47% in all PD patients. Prion proteins (PrPs), which are expressed in both neurons and glial cells of the brain, are believed to be correlated with abnormal neurological ... more

W.J. Zhang; X.L. Shang; J. Peng; M.H. Zhou; W.J. Sun
02/08/2017
alpha-Fetoproteins; Animals; Disease Models, Animal; Gene expression; Glypicans; Humans; Liver neoplasms; male; Neoplasm Micrometastasis; Polymerase chain reaction; Rats; Rats, Sprague-Dawley; RNA, Messenger

The incidence of liver cancer has gradually risen to a high level in China, and tumor metastasis occurs via multiple pathways. Alpha fetal protein (AFP) is the main biomarker of liver cancer micrometastases. A recent study showed that glypican-3 (GPC3), which is abundant in hepatoma cells, has promising specificity and could be used to determine the presence of malignant cells. The nested ... more

J. Luo; K. Yang; Y.G. Wen
02/16/2017
Cell differentiation; Colorectal neoplasms; Down-regulation; Female; Gene Expression Regulation, Neoplastic; HT29 Cells; Humans; Kruppel-Like Transcription Factors; Lymphatic metastasis; male; Prognosis; Survival analysis

Kruppel-like factors (KLFs) are a group of transcriptional regulators that have recently been identified to exhibit tumor-suppressive function against various gastrointestinal cancers. The present study aims to investigate the expression patterns and prognostic value of KLF-4 in colorectal cancers (CRCs). KLF-4 levels in CRC tissues were examined via immunohistochemistry analysis, real-time ... more

D.H. Xiu; Y. Chen; L. Liu; H.S. Yang; G.F. Liu
2017 Feb 16
Adolescent; Antineoplastic Agents; Arsenic Trioxide; Arsenicals; Cerebellar Neoplasms; Child; Child, Preschool; Gene Expression Regulation, Neoplastic; Hedgehog Proteins; Humans; Infant; Medulloblastoma; Oxides; Signal transduction

Recent innovations in the genomic understanding of medulloblastomas have provided new ways to explore this highly invasive malignant brain cancer arising from the cerebellum. Among the four different medulloblastoma subgroups described to date, the sonic hedgehog (SHH) genetic pathway is the pathway activated in the tumorigenesis of medulloblastoma. SHH-related medulloblastomas are usually of ... more

P.H.S. Klinger; A.F. Andrade; L.E.A. Delsin; R.G.P. Queiroz; C.A. Scrideli; L.G. Tone; E.T. Valera
2017 Mar 16
Adult; Aged; Down-regulation; Factor VIII; Female; Gene Expression Regulation, Neoplastic; GTPase-Activating Proteins; Humans; Lymphatic metastasis; male; Middle Aged; Neoplasm Grading; Neoplasm Invasiveness; Nuclear Proteins; Stomach neoplasms; Vascular Endothelial Growth Factor A

Association of signal-induced proliferation-associated 1 (SIPA) gene and protein expression with gastric cancer development was examined. SIPA1 mRNA and protein levels were determined by real-time quantitative polymerase chain reaction and western blot, respectively, in 40 gastric tumor and tumor-adjacent normal tissues. SIPA1, VEGF-A, and FVIII levels in 60 gastric tumor and 40 tumor-adjacent ... more

J.Y. Li; J.B. Wang; C.B. Liu; D.L. Ma; J.H. Ma

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