Research Article

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For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous to the MT type 2 (isoform 1), Mt2, and Mt3 sequences of other plant ... more

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9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) ... more

A.M. Pedrosa; L.C. Cidade; C.P.S. Martins; A.F. Macedo; D.M. Neves; F.P. Gomes; E.I.S. Floh; M.G.C. Costa
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Disease resistance; Fusarium; Gene Expression Regulation, Plant; Lycopersicon esculentum; Plant Proteins; Plant Roots; Proteome; Stress, Physiological

Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by ... more

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Azospirillum brasilense; Expressed sequence tags; Genotype; Glutamate-Ammonia Ligase; Nitrate reductase; Nitrogen; Plant Proteins; Zea mays

The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised ... more

L. Pereira-Defilippi; E.M. Pereira; F.M. Silva; G.V. Moro
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Basic-Leucine Zipper Transcription Factors; Edible Grain; Homeostasis; Plant breeding; Plant Proteins; Zinc

Members of the basic leucine zipper family, as bZIP19, are considered to be essential regulators of the adaptation to zinc deficiency. Knowing that this gene as well as its targets are conserved in the plant kingdom, we followed an in silico approach to identify and characterize the bzip19 gene in cereals. Through BLASTp in Phytozome database, 33 bzip19 genes were identified on the genomes of ... more

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Fusarium; Gene Expression Regulation, Plant; Genetic markers; Genetic variation; Heat-shock proteins; Inbreeding; Lipoxygenase; maternal inheritance; Plant breeding; Plant Immunity; Plant Proteins; Selection, Genetic; Zea mays

In recent years, there has been a large incidence of fungi causing "ear rot" in maize in Brazil, the main fungus being Fusarium verticillioides. The most efficient and competitive alternative for control of this disease consists of using maize hybrids resistant to this pathogen. Thus, the aims of this study were to analyze the genetic variability of maize inbred lines in regard to resistance ... more

G.S. Pereira; R.G.V. Pinho; E.V.R.V. Pinho; L.P.M. Pires; L.A.Y.Bernar Junior; J.L.A. Pereira; M.P. Melo
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Amino Acid Motifs; Cloning, Molecular; Droughts; Hordeum; Plant Proteins; Protein domains; Qa-SNARE Proteins; Stress, Physiological

Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, "Himalaya 10"; its expression was evaluated during drought stress and rehydration via real-time PCR. The cloned HbSYR1 ... more

Q.J. Xu; Y.L. Wang; Z.X. Wei; H.J. Yuan; X.Q. Zeng; N. Tashi
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Genome, Plant; Iron; Oryza; Phenotype; Plant Proteins; Stress, Physiological; Transcription factors

Iron (Fe) is an essential microelement for all living organisms playing important roles in several metabolic reactions. Rice (Oryza sativa L.) is commonly cultivated in paddy fields, where Fe goes through a reduction reaction from Fe to Fe. Since Fe is more soluble, it can reach toxic levels inside plant cells, constituting an important target for studies. Here we aimed to verify morphological ... more

V.E. Viana; N. Marini; T. Finatto; I. Ezquer; C. Busanello; R.S.Dos Santos; C. Pegoraro; L. Colombo; C. de Oliveira
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Arachis; Genotype; Plant Leaves; Plant Proteins; Resveratrol; RNA, Messenger; Stilbenes; Tetraploidy; Transcriptional activation; Ultraviolet Rays

Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes ... more

P.A.S.V. Carvalho; A.C. Brasileiro; S. Leal-Bertioli; D.J. Bertioli; J.P. Silva; T.S. Agostini-Costa; M.A. Gimenes
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Anti-Bacterial Agents; Antifungal Agents; Carbon Dioxide; Flowers; Plant Extracts; Solanaceae

Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon ... more

L.C.T. Thiesen; E. Y Y Sugauara; V. Tešević; J. Glamočlija; M. Soković; J.E. Gonçalves; Z.C. Gazim; G.A. Linde; N.B. Colauto

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