Research Article

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2017 Jan 23
Breeding; Crosses, Genetic; Disease resistance; Genes, Plant; Genetic markers; Microsatellite Repeats; Oryza; Plant Diseases

Rice (Oryza sativa L.) blast disease is one of the most destructive rice diseases in the world. The fungal pathogen, Magnaporthe oryzae, is the causal agent of rice blast disease. Development of resistant cultivars is the most preferred method to achieve sustainable rice production. However, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. ... more

N. Hasan; M.Y. Rafii; A. Rahim; S.A. Nusaibah; N. Mazlan; S. Abdullah
2017 Jan 23
Gene expression; Genes, Plant; Genetic Vectors; Ginsenosides; Panax; Plants, Genetically Modified; Transformation, Genetic

Panax japonicus C.A. Meyer, a perennial herb belonging to the Araliaceae ginseng genus, is one of the seven rare and endangered Chinese medical herbs. By cloning the SS segment, the expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and introduced into Agrobactria LBA4404, which is used for engineering bacteria. Polymerase chain reaction (PCR) showed that the PjSS, antiPjSS, and ... more

L. Zhang; T.T. Wang
2017 Mar 15
Genes, Plant; Genetic variation; Phenotype; Plant breeding; Saccharum; Selection, Genetic; Sucrose

Sugarcane breeding programs have been adapting to a new market demand: aside from high sucrose yield per hectare, the sector needs new cultivars with higher fiber percentages. The selection of sugarcane clones based on phenotype alone is a complex task. The selected clones should display high performance in a series of yield- and quality-related traits. Selection indices can provide ... more

A.A.C. de Azeredo; L.L. Bhering; B.P. Brasileiro; C.D. Cruz; L.C.I. Silveira; R.A. Oliveira; J.C.Bespalho Filho; E. Daros
2017 Apr 20
Capsicum; Genes, Plant; Genetic variation; Plant Immunity; Xanthomonas

Since 2008, Brazil is the largest consumer of agrochemicals, which increases production costs and risks of agricultural products, environment, and farmers' contamination. Sweet pepper, which is one of the main consumed vegetables in the country, is on top of the list of the most sprayed crops. The bacterial spot, caused by Xanthomonas spp, is one of the most damaging diseases of pepper crops. ... more

L.R.A. Silva; R. Rodrigues; S. Pimenta; J.W.S. Correa; M.S.B. Araújo; C.S. Bento; C.P. Sudré
2017 Aug 17
Alleles; Genes, Plant; Microsatellite Repeats; Plant breeding; Polymerase chain reaction; Reference Standards; Triticum

One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in genetic breeding programs besides the loss of valuable genetic material ... more

H.N. Heim; E.S.N. Vieira; L.R.T. Polo; N.K. Lima; G.J. Silva; G.A. Linde; N.B. Colauto; I. Schuster
2016 Dec 02
Alcohol Oxidoreductases; Anthocyanins; Cloning, Molecular; Flavonols; Fruit; Gene Expression Regulation, Plant; Glucosyltransferases; Organ Specificity; Plant Proteins; Transcription factors; Vitis

MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and ... more

T.Q. Niu; Z.D. Gao; P.F. Zhang; X.J. Zhang; M.Y. Gao; W. Ji; W.X. Fan; P.F. Wen
2016 Dec 19
Cloning, Molecular; Cytokinins; Gene Expression Regulation, Plant; Lactones; Plant Proteins; Tobacco; Transcription factors

Axillary branching is controlled by a very complex mechanism involving various endogenous and environmental factors. Previous studies have shown that Tb1/BRC1 is the point of integration in the network of molecular mechanisms regulating axillary branching in plants. In this study, we cloned the Tb1/BRC1 ortholog, NtBRC1, from Nicotiana tabacum and functionally analyzed its role in the control ... more

Y.L. Gao; Z.B. Song; W.Z. Li; F.C. Jiao; R. Wang; C.J. Huang; Y.P. Li; B.W. Wang
2017 Jan 23
Animals; Lymphocytes; Mice; Oryza; Plant Proteins; Plants, Genetically Modified

With the expanding demand for genetically modified (GM) rice, its safety evaluation is of great significance. Therefore, this study was carried out to assess the acute cytotoxicity of the whole protein extract from GM rice Bar68-1 in Mus musculus lymphocytes in vitro. Cell viability was determined by Cell Counting Kit-8 (CCK-8) and Neutral Red Uptake (NRU) tests. CCK-8 tests was carried out ... more

X.B. Lu
2017 Feb 08
Chitinases; Cloning, Molecular; Cucurbitaceae; Gene expression; Penicillium; Plant Proteins; Reverse Transcriptase Polymerase Chain Reaction; Time Factors

Chitinases are important disease-related proteins that play critical roles in plant defense against disease. To investigate the function of chitinases in the resistance of Hami melon to Penicillium infection, the gene encoding chitinases, HmCHT-2, was cloned and RT-PCR was used to measure expression levels of HmCHT-2. When the Hami melon was infected by Penicillium sp after 0, 12, 36, 48, 60, ... more

F.Y. Jiang; B.B. Shui; F.X. Tang; C.H. Shan
2017 Feb 08
Fruit; Meliaceae; Plant Extracts; Plant Proteins; Proteomics

We investigated the extraction of Toona sinensis fruit proteins and preliminarily characterized their physicochemical properties. The results showed that optimal extraction occurred under conditions of pH 10.5, a duration of 40 min, a liquid-to-solid ratio of 25:1, and a temperature of 40°C by an orthogonal design using T. sinensis fruit protein as the index and single factor. The total ... more

W.Z. Li; W.N. Han; B. Liu; S.H. Ding; X.K. Zhang; R.S. Wang

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