Research Article

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2017 Sep 27
Animals; Gene Expression Regulation, Developmental; Rabbits; Retina; RNA, Messenger; Rod Opsins; Thy-1 Antigens

Melanopsin is the photopigment of intrinsically photosensitive retinal ganglion cells that mediate non-visual responses to light. The aim of this study was to describe and analyze melanopsin gene expression in the rabbit retina at different ages and compare its expression with the prototypic gene of retinal ganglion cells (Thy-1 gene). Expression levels of OPN4, Thy-1, and GADPH genes were ... more

M.M. Lira-Carrera; B.E. Gutiérrez-Amavizca; L.J. Álvarez-Araujo; M. Aguirre-Ramírez; J.A. Pérez-León
Aquaculture; Babylonia areolata; Microsatellites; Population genetics; Spotted babylon

The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per ... more

Y. Wang; H. Lu; J. Zheng; K. Long; Y.H. Shi; Z.F. Gu; A.M. Wang
Abalone; Aquaculture; Haliotis diversicolor; Microsatellite

The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an ... more

Y. Wang; F. Wang; Y.H. Shi; Z.F. Gu; A.M. Wang
454 Sequencing; Aquaculture; Bioinformatics; Haliotis midae; Molecular markers

Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and ... more

R. Slabbert; J.A. Hepple; C. Rhode; A.E.Bester Van der Merwe; R. Roodt-Wilding
Aquaculture; Golden mandarin fish; Microsatellite markers; Selective breeding

In this study, 12 polymorphic microsatellites were inves­tigated to determine the genetic diversity and structure of 5 consecu­tive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breeding populations was ... more

X.N. Luo; M. Yang; X.F. Liang; K. Jin; L.Y. Lv; C.X. Tian; Y.C. Yuan; J. Sun
cDNA cloning; Chinese perch; Fasting-re-feeding; Gene expression; Myostatin

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including an open-reading frame of 1131 bp, which encoded 376 amino acids. Sequence ... more

L. Liu; Y.L. Li; S.D. Xu; K.Z. Wang; P. Wu; W.Y. Chu; X.Q. Wang; L. Liu; Y.L. Li; S.D. Xu; K.Z. Wang; P. Wu; W.Y. Chu; X.Q. Wang
Calpastatin; meat quality; Meat tenderness; Myostatin; PCR-SSCP

In Brazil, most sheep are of the Santa Inês breed, which shows rusticity in breeding and grazing systems, being able to offer better adaptation characteristics for animals with meat production ability, such as the Texel and Dorper sheep. They can also improve meat production in animals that have better characteristics for milk production, such as Lacaune and East ... more

C. Esteves; K.Gdo Livramento; L.V. Paiva; A.P. Peconick; I.F.F. Garcia; C.A.P. Garbossa; T.B. Cardoso; P.B. Faria
Gene expression; Hypoxia; Muscle; Pig

Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression level of four hypoxia-related genes ( ... more

J. Zhang; L. Chen; K.R. Long; Z.P. Mu
Gene expression; Hyperplasia and hypertrophy; Megalobrama amblycephala; Morphological analysis; Muscle fiber

Skeletal muscle growth is regulated by both positive and negative factors, such as myogenic regulatory factors (MRFs) and myostatin (MSTN), and involves both hyperplasia and hypertrophy. In the present study, morphological changes during muscle development in Megalobrama amblycephala were characterized and gene expression levels were measured by quantitative real-time polymerase chain ... more

K.C. Zhu; D.H. Yu; J.K. Zhao; W.M. Wang; H.L. Wang
Annual Medicago; Gene expression; Microarray analysis; Salinity

The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive ... more

F. Gharaghani; F. Rafiei; N. Mirakhorli; E. Ebrahimie