Research Article

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12/02/2016
Animals; Body weight; Cattle; Genome-wide association study; Genotype; Models, Biological; Phenotype; Polymorphism, Single Nucleotide; Quantitative trait loci

This study aimed to investigate the predictability of a phenotype when using a dynamic model of cattle growth. Genotypic and phenotypic information on Nellore (Bos indicus) cattle were used in a genome-wide association analysis designed to contrast the biological interpretation of core parameters [conversion efficiency of metabolizable energy to net energy for gain (k) and adjusted final ... more

M.C. Freua; M.H.A. Santana; R.V. Ventura; J.B.S. Ferraz
12/02/2016
Administration, Oral; Animals; Bone marrow cells; Cell proliferation; Cells, Cultured; Guatemala; Humans; Lymphocytes; male; Mexico; Micronucleus tests; Mutagenicity Tests; Plant Extracts; Rats; Rubus; Sister chromatid exchange; Toxicity Tests, Subchronic

Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of ... more

S. González-Hernández; D. González-Ramírez; M.I. Dávila-Rodríguez; A. Jimenez-Arellanez; M. Meckes-Fischer; S. Said-Fernández; E.I. Cortés-Gutiérrez
12/02/2016
Animals; Brazil; Breeding; Cattle; Gene frequency; Genetic markers; Leptin; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Red Meat; Sequence Analysis, DNA

Brazil is the world's largest producer of beef cattle; however, the quality of its herds needs to be improved. The use of molecular markers as auxiliary tools in selecting animals for reproduction with high pattern for beef production would significantly improve the quality of the final beef product in Brazil. The leptin gene has been demonstrated to be an excellent candidate gene for bovine ... more

R.C. Guimarães; J.S.N. Azevedo; S.C. Corrêa; J.E.G. Campelo; E.M. Barbosa; E.C. Gonçalves; S. Filho
12/02/2016
Animals; Citalopram; Dementia, Vascular; Disease Models, Animal; Dopamine; Gene Expression Regulation; Hippocampus; Humans; Injections, Intraperitoneal; male; Neurons; Norepinephrine; Random Allocation; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT1A; Selective Serotonin Reuptake Inhibitors; Serotonin; Treatment outcome

5-hydroxytryptamine receptor 1A (5-HT1AR) is closely associated with cognitive functions. Selective serotonin reuptake inhibitors (SSRIs) can protect individuals from brain damage following ischemia/hypoxia. To investigate the function of SSRIs in vascular dementia (VD), we established a rat model of VD, and observed the effect of SSRIs on the expression of 5-HT1AR mRNA and neurotransmitters. ... more

K. Guo; G. Yin; X.H. Zi; H.X. Zhu; Q. Pan
12/02/2016
Animals; Apoptosis; Cells, Cultured; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Gonadotropins, Equine; Horses; In Vitro Oocyte Maturation Techniques; Oocytes; Receptors, FSH; Receptors, LH; Receptors, LHRH; Sheep

We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM ... more

S.C. Wei; Z.D. Gong; H.W. Zhao; H.Q. Liang; L.J. Lai; Y.Y. Deng
12/02/2016
Animals; Bayes Theorem; Biological evolution; Butterflies; Evolution, Molecular; Gene order; Genome, Insect; Genome, Mitochondrial; Likelihood Functions; Phylogeny

Pieridae is a butterfly family whose evolutionary history is poorly understood. Due to the difficulties in identifying morphological synapomorphies within the group and the scarcity of the fossil records, only a few studies on higher phylogeny of Pieridae have been reported to date. In this study, we describe the complete mitochondrial genomes of four pierid butterfly species (Aporia martineti ... more

Y. Cao; J.S. Hao; X.Y. Sun; B. Zheng; Q. Yang
12/02/2016
Animals; Avian Proteins; Chickens; Female; Genetic association studies; Genotype; Insulin-like growth factor I; Phenotype; Polymorphism, Single-Stranded Conformational; Quantitative trait loci; Reproduction; Selection, Genetic

The insulin-like growth factor, IGF-I, plays an important role in the development of growth and reproductive traits. Single-strand conformation polymorphism analysis was used to detect and analyze polymorphisms and expression profiles of the IGF-I gene and its association with growth and reproductive traits of Jinghai yellow chickens. A point mutation g.295T>C was detected in the IGF-I gene ... more

M.A. Abdalhag; T. Li; L. Duan; T. Zhang; G. Zhang; J. Wang; Y. Wang
12/19/2016
Animals; Antimutagenic Agents; Antioxidants; Chromosome aberrations; Cyclophosphamide; Fruit; male; Malpighiaceae; Models, Animal; Plant Extracts; Rats; Rats, Wistar

Barbados cherry (BC) (Malpighia glabra Linnaeus) is a functional fruit that is consumed to prevent disease. It is used as an adjuvant in the treatment of several diseases, and acts as an antianemic, an appetite stimulant, a wound healer, an anti-inflammatory, a mineralizer, an antifungal, and an antioxidant agent. Several chemotherapeutic agents, such as cyclophosphamide, may result in ... more

E. Düsman; I.V. Almeida; L.T.D. Tonin; V.E.P. Vicentini
12/19/2016
Animals; Cell Line, Tumor; Cell Movement; Cell proliferation; Endoglin; Female; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Nude; MicroRNAs; Neoplasm Invasiveness; Neoplasm Transplantation; Neovascularization, Pathologic; Thyroid Neoplasms

Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in thyroid cancer cells. A wound healing assay was adopted to examine ... more

Y.L. Wang; W.G. Gong; Q.L. Yuan
12/19/2016
Animals; Feces; Foxes; Genetic markers; Genotyping Techniques; Heterozygote; Microsatellite Repeats

The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 ... more

T. Tada; Y. Seki; Y. Kameyama; Y. Kikkawa; K. Wada

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