Research Article

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3/30/2017
Alleles; Animals; Brazil; Breeding; Chromosome Mapping; DNA; Genetic Drift; Genetic variation; Genetics, Population; Genotype; Goats; Heterozygote; Microsatellite Repeats

The aim of this study was to access the genetic diversity and relatedness between Canindé and British Alpine goat breeds in the States of Piauí and Ceará using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype individuals. The microsatellite markers ... more

T.S. Câmara; J.F. Nunes; F.M. Diniz; G.R. Silva; A.M. Araújo
4/05/2017
Animals; Antibiosis; Brazil; Cucumis melo; Diptera; Genetic variation; Insect Repellents; Larva; Pest Control, Biological

Leafminers (Liriomyza sativae) are the main melon (Cucumis melo L.) pests in Northeast Brazil, which is the main region for the production and export of the fruit in Brazil. Of the integrated management strategies available, genetic resistance is the best method of preventing damage by these insects. The aim of this study was to select sources of resistance to leafminers in melon germplasm. ... more

E.F. Celin; F.I.C. Oliveira; N.S. Dias-Pini; G.H.S. Nunes; F.A.S. Aragão
4/05/2017
Animals; Brazil; Cattle; Cattle Diseases; Coronavirus Infections; Coronavirus, Bovine; Dysentery; Evolution, Molecular; Feces; Membrane Glycoproteins; Phylogeny; Sequence Analysis, DNA; Viral Envelope Proteins; Viral Tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of respiratory disease in ... more

E.A. Beuttemmuller; A.F. Alfieri; S.A. Headley; A.A. Alfieri
09/21/2017
Animals; Brazil; Dogs; Feces; Genome, Viral; Parvoviridae Infections; Parvovirus, Canine; Phylogeny; Polymorphism, Genetic; Recombination, Genetic

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was ... more

S.P. Silva; L.N.P.P. Silva; E.D.L. Rodrigues; J.F. Cardoso; F.N. Tavares; W.M. Souza; C.M.P. Santos; F.M.S. Martins; I.S. Jesus; T.C. Brito; T.P.C. Moura; M.R.T. Nunes; L.M.N. Casseb; S. Filho; A.R. Casseb
1/23/2017
Animals; Lymphocytes; Mice; Oryza; Plant Proteins; Plants, Genetically Modified

With the expanding demand for genetically modified (GM) rice, its safety evaluation is of great significance. Therefore, this study was carried out to assess the acute cytotoxicity of the whole protein extract from GM rice Bar68-1 in Mus musculus lymphocytes in vitro. Cell viability was determined by Cell Counting Kit-8 (CCK-8) and Neutral Red Uptake (NRU) tests. CCK-8 tests was carried out ... more

X.B. Lu
1/23/2017
Animals; Biomarkers; Cell line; G-Protein-Coupled Receptor Kinase 2; Humans; Mechanical Phenomena; Mice; Osteoblasts; Phosphorylation; Receptors, Purinergic P2Y2; Signal transduction

Bone desensitization after mechanical loading is essential for bone to adapt to its mechanical environment. However, the desensitization mechanism is unknown. Previous studies suggest that G protein-coupled receptors (GPCRs), including P2Y and parathyroid hormone receptors, play important roles in osteoblast mechanobiology. Thus, for the present research, we examined the role of G protein- ... more

Y. Xing; Y. Gu; X. Shan; L. Wang; J. You
05/10/2017
Adipose tissue; Animals; Antioxidants; Body weight; Camellia sinensis; Chromosomal Proteins, Non-Histone; Chronotherapy; Circadian rhythm; Diet; Eating; Female; Homeostasis; Lipid metabolism; Liver; Liver X Receptors; Mice; Ovary; Oxidation-Reduction; Plant Extracts; Tea

Biological rhythms can be defined as changes in physiological or behavioral variables that repeat at certain time intervals. Rhythms that last approximately 24 h are referred to as circadian rhythms. Modern lifestyles have drastically affected human habits, as well as the population's eating habits. These changes have generated an epidemic of metabolic syndromes, such as obesity and diabetes. ... more

R.P. Amorim; I.P. Daher; F.P.D. Arcangelo; M.F. Bellini; W.A. Orcini; G.R. Sarria; R.L. Peruquetti
05/10/2017
Amyotrophic lateral sclerosis; Animals; Cell line; DNA-Binding Proteins; MafK Transcription Factor; Mice; Mutation, Missense; NAD(P)H Dehydrogenase (Quinone); Neurons; NF-E2-Related Factor 2; Oxidative stress; Repressor Proteins; Response Elements

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that affects motor neurons and lacks an effective treatment. The disease pathogenesis has not been clarified at present. Pathological transactive response DNA-binding protein 43 (TDP-43) plays an important role in the pathogenesis of ALS. Nuclear translocation of nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) is ... more

Y.P. Tian; F.Y. Che; Q.P. Su; Y.C. Lu; C.P. You; L.M. Huang; S.G. Wang; L. Wang; J.X. Yu
09/27/2017
Animals; Anovulation; Brain; Female; Interleukin-6; Leptin; Lipid metabolism; Mice; Mice, Obese; Receptor Protein-Tyrosine Kinases; Receptors, GABA; Receptors, Muscarinic; Receptors, Serotonin; Synaptic Transmission

Polycystic ovary syndrome is the classic example of loss of functional cyclicity and anomalous feedback. In this case, the excessive extra-glandular production and conversion of androgens to estrogens are the pathophysiological basis of the chronic anovulation. The literature describes an experimental model of the polymicrocystic ovary in obese diabetic mice with insulin resistance. The fact ... more

M.A. Azevedo; I.D.C.G. Silva
2/16/2017
Animals; Gene expression profiling; Gene Expression Regulation; Genetic markers; Oligonucleotide Array Sequence Analysis; Phenotype; Quantitative trait loci; Sheep; Wool

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provide a molecular basis for improving wool quality in sheep. Genes ... more

Y.Z. Tian; T. Usman; K.C. Tian; J. Di; X.X. Huang; X.M. Xu; H. Tulafu; W.W. Wu; X.F. Fu; Y. Bai; M. Tuerxun; A. Maimaiti

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