Research Article

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05/31/2017
Animals; Carbohydrates; Cell Line, Tumor; Cell proliferation; DNA damage; Food Handling; Food Quality; Food storage; Fruit and Vegetable Juices; Rats; Ultraviolet Rays; Vitamins; Vitis

Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) ... more

E. Düsman; I.V. Almeida; E.P. Pinto; L. Lucchetta; V.E.P. Vicentini
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Animals; Apoptosis; Cells, Cultured; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Gonadotropins, Equine; Horses; In Vitro Oocyte Maturation Techniques; Oocytes; Receptors, FSH; Receptors, LH; Receptors, LHRH; Sheep

We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM ... more

S.C. Wei; Z.D. Gong; H.W. Zhao; H.Q. Liang; L.J. Lai; Y.Y. Deng
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Antibiotics, Antineoplastic; Apoptosis; Bleomycin; Cell line; Endoplasmic Reticulum Chaperone BiP; Endoplasmic reticulum stress; Heat-shock proteins; Human Umbilical Vein Endothelial Cells; Humans; Receptors, G-Protein-Coupled; Transcription Factor CHOP

In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK- ... more

Y.D. Huang; X. Tong; Q. Chen; S.W. Xia; Y. Zhuo; Z.H. Kang; X.Y. Xue
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Alzheimer disease; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Gene Expression Regulation; Hippocampus; Humans; Maze Learning; Mice; MicroRNAs; Receptor, Cannabinoid, CB2

Alzheimer's disease (AD) is a neurodegenerative disorder, and is the most common type of dementia in the elderly population. Growing evidence indicates that microRNAs (miRNAs) play a crucial role in neuroinflammation associated with AD progression. In this study, we analyzed the expression of microRNA-139 (miR-139) as well as the learning and memory function in AD. We observed that the miR-139 ... more

Y. Tang; J.S. Bao; J.H. Su; W. Huang
08/17/2017
Cells, Cultured; DNA damage; Euterpe; Fruit; Hep G2 Cells; Humans; Lymphocytes; Plant Oils

Euterpe oleracea Mart., popularly known as "açaí", is a tropical fruit from the Amazon region where it has considerable economic importance. Açaí has been used as food and for several medicinal purposes. Despite the widespread use of this fruit, there is a lack of data regarding the safety of using this fruit oil exclusively. Therefore, we evaluated the in vitro cytotoxic, genotoxic, and ... more

E.S. Marques; M.S.F. Tsuboy; J.C.T. Carvalho; P.C.P. Rosa; F.F. Perazzo; I.O.M. Gaivão; E.L. Maistro
07/31/2015
Bone marrow mesenchymal stem cells; Hepatic stellate cells; TGF-b1/Smad pathway

This study investigated the effect of bone marrow mesenchymal stem cells (BMCs) on the transforming growth factor-β1 (TGF-β1)-induced activation of the Smad signaling pathway in rat hepatic stellate cells (HSCs). There were four experimental groups: 1) a blank control group, 2) a TGF-β1 treatment group, 3) an MSC-combined group, and 4) an induced MSC-combined group. Isolation ... more

X.E. Sun; X.Q. Zhang; M.M. Liu
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a-Smooth muscle actin; Glucose; Hepatic stellate cells; p38-Mitogen-activated protein kinase signaling pathway; SB203580

The relationship between the p38-mitogen-activated protein kinase (p38-MAPK) signal pathway and high glucose-induced hepatic stellate cell (HSC) activation was investigated in this study. Sixty human HSC samples were randomly selected and used in the control (cultured normally), high-glucose (cultured in the presence of high glucose), and blocking (cultured under high-glucose conditions ... more

L.K. Wu; Y.C. Liu; G. Ma; L.L. Shi; X.M. He; L.K. Wu; Y.C. Liu; G. Ma; L.L. Shi; X.M. He
07/13/2015
Bone morphogenetic protein 9; Cell differentiation; Mesenchymal stem cells; Mitogen activated protein kinase; Signal transduction pathway

We investigated the synergistic effect of bone morphoge­netic protein 9 (BMP9) and transforming growth factor (TGF)-b in the transformation of mesenchymal stem cells into osteoblasts. We evalu­ated the effect of BMP9 and TGF-b on the induction of osteoblast for­mation. Mitogen-activated protein kinase (MAPK) pathway-related pro­teins such as p38, extracellular receptor kinase 1/2, and c- ... more

X.L. Li; Y.B. Liu; E.G. Ma; W.X. Shen; H. Li; Y.N. Zhang
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Long non-coding RNA; Mesenchymal stem cells; Osteogenic differentiation

The aim of this study was to identify long non-coding RNA (lncRNA) associated with osteogenic differentiation from mesenchymal stem cells (MSCs) using high-throughput RNA sequencing (RNA-Seq) data. RNA-Seq dataset was obtained from the European Bioinformatics Institute (accession No. PRJEB4496), including two replicates each for immortalized mesenchymal stem cells iMSC#3 cultured in ... more

W.Q. Song; W.Q. Gu; Y.B. Qian; X. Ma; Y.J. Mao; W.J. Liu
12/28/2015
Mesenchymal stem cells; Runt-related transcription factor 2; RUNX2; Stem cell differentiation

Past studies have revealed the critical role of runt-related transcription factor 2 (RUNX2) in the proliferation and differentiation of mesenchymal stem cells (MSCs). This study therefore aimed to investigate the expression profile of the RUNX2 gene in human bone marrow MSCs and its biological characteristics. Bone marrow MSCs were separated from 12 patients who had received hip ... more

J.Y. Zhang; L.C. Li

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