Research Article

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4/13/2017
Animals; Gene frequency; Heterozygote; Humans; Polymorphism, Genetic; Software

The gene diversity or expected heterozygosity (H) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of H can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a ... more

A.R. da Silva; G. Malafaia; I.P.P. Menezes
4/28/2017
Dipteryx; Gene frequency; Genetic markers; Genotype; High-Throughput Nucleotide Sequencing; Microsatellite Repeats; Polymorphism, Genetic; Sequence Analysis, DNA

The use of next-generation sequencing (NGS) technologies provides a great volume of genome sequence data even for non-model species. The development of microsatellite markers using these data is a relatively quick and easy process. Dipteryx alata Vogel (Fabaceae) is an arboreal species from the Cerrado biome and is considered an important plant genetic resource. Here, we report the development ... more

R.A. Guimarães; M.P.C. Telles; A.M. Antunes; K.M. Corrêa; C.V.G. Ribeiro; A.S.G. Coelho; T.N. Soares
5/04/2017
Animals; Aquaculture; Gene frequency; Genotype; HSP70 Heat-Shock Proteins; Penaeidae; Polymorphism, Single Nucleotide

Single nucleotide polymorphisms (SNPs) are the best genetic markers for associative studies of the immune system in invertebrates. In the marine shrimp Litopenaeus vannamei, SNPs linked to disease resistance have been reported for some genes, such as hemocyanin, anti-lipopolysaccharide factor, and heat-shock protein 70 (Hsp70). In the present study, polymorphisms in the Hsp70 gene were ... more

A.L. Ferreira; R. Maggioni; D. Conceição; L.M. Perazzolo; R.L. Petersen
05/25/2017
Animals; Buffaloes; Female; Fertility; Gene frequency; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Receptors, Melatonin

Buffalo production is spreading globally because of its economic advantage. Then, it has become necessary to improve the reproductive and productive efficiency of these animals, as well as to look for genetic factors that increase this efficiency. The objectives of this study were to characterize the promoter region of the melatonin 1A receptor gene (MTRN1A), to detect possible SNPs and ... more

E.M. Barbosa; B.B. Souza; R.C. Guimarães; L.K.N. Silva; J.S.N. Azevedo; E.C. Gonçalves; H.F.L. Ribeiro; S.T.Rolim Filho; S. Filho
08/17/2017
Eucalyptus; Gene frequency; Genotype; Linkage disequilibrium; Microsatellite Repeats; Polymorphism, Genetic

Eucalyptus urophylla is an important species in the Brazilian forest sector due to its rapid growth rates and resistance to disease. The aim of this study was to verify Mendelian inheritance, genetic linkage, and genotypic disequilibrium for 15 microsatellite loci, with the goal of producing a robust set of genetic markers. Mendelian inheritance and genetic linkage analyses were carried out ... more

S. Pupin; L.N. Rosse; I.C.G. Souza; J. Cambuim; C.L. Marino; M.L.T. Moraes; A.M. Sebbenn
09/27/2017
Arecaceae; environment; Gene frequency; Genotype; Microsatellite Repeats; Phenotype; Polymorphism, Genetic; Selection, Genetic

This study uses ISSR molecular markers to characterize the demographic pattern, and spatial genetic structure (SGS) at different life stages of development (cohorts) in a natural population of Copernicia prunifera in the Rio Grande do Norte State, Brazil. All individuals were sampled and georeferenced in a 0.55-ha plot. The demographic analyses showed a clustered pattern in the first-distance ... more

L.G. Pinheiro; K.P.T. Chagas; A.S.M. Freire; M.C. Ferreira; C.G. Fajardo; F.A. Vieira
09/27/2017
Ecuador; Gene frequency; Humans; Microsatellite Repeats; Polymorphism, Genetic; Population

One hundred and eighty-two samples of unrelated people who requested the paternity test at the Molecular Biology and Genetics Laboratory of the Catholic University of Cuenca-Ecuador in the province of Azuay were studied, except for the D1S1656 (180 samples) and SE33 (89 samples) markers. The STRs D22S1045, D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D1S1656, ... more

P.P. Orellana; C.F. Andrade; C.L. Arciniegas; G.C. Iannacone
12/02/2016
Animal Shells; Animals; Bivalvia; Breeding; Evolution, Molecular; Female; Fresh Water; Genetic variation; Genetics, Population; male; Microsatellite Repeats; Phenotype; Phylogeny; Quantitative trait loci

Four Hyriopsis cumingii populations, a breeding population (BP), a cultured population (FP), two wild populations from Poyang Lake (PY) and Dongting Lake (DT), and an H. schlegelii population were collected (JX), and the first filial generations (F1) were bred synchronously. The shell nacre polymorphisms, population genetic diversity, and genetic structures of the F1 of each population were ... more

L.M. Wu; Z.Y. Bai; X.J. Liu; C. Jin; H. Yin; J.L. Li
12/02/2016
Azoospermia; Chromosome aberrations; Cytogenetic Analysis; Genetic counseling; Heterozygote; Humans; Live Birth; male; Oligospermia

Chromosomal abnormality is the most common genetic cause of infertility. Infertility, as a psychological problem, has received an increasing amount of attention. Psychological interventions have been shown to have beneficial effects on infertile patients with chromosomal abnormalities. The present study explored reproductive outcome of male carriers of chromosomal abnormalities, who accepted ... more

K.M. Guo; B. Wu; H.B. Wang; R.H. Tian
12/02/2016
Administration, Oral; Animals; Bone marrow cells; Cell proliferation; Cells, Cultured; Guatemala; Humans; Lymphocytes; male; Mexico; Micronucleus tests; Mutagenicity Tests; Plant Extracts; Rats; Rubus; Sister chromatid exchange; Toxicity Tests, Subchronic

Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of ... more

S. González-Hernández; D. González-Ramírez; M.I. Dávila-Rodríguez; A. Jimenez-Arellanez; M. Meckes-Fischer; S. Said-Fernández; E.I. Cortés-Gutiérrez

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