Research Article

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2017 Mar 30
Abscisic acid; Adaptation, Physiological; Citrus; Dioxygenases; Droughts; Nicotiana; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Stress, Physiological

9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) ... more

A.M. Pedrosa; L.C. Cidade; C.P.S. Martins; A.F. Macedo; D.M. Neves; F.P. Gomes; E.I.S. Floh; M.G.C. Costa
09/27/2017
Genome, Plant; Iron; Oryza; Phenotype; Plant Proteins; Stress, Physiological; Transcription factors

Iron (Fe) is an essential microelement for all living organisms playing important roles in several metabolic reactions. Rice (Oryza sativa L.) is commonly cultivated in paddy fields, where Fe goes through a reduction reaction from Fe to Fe. Since Fe is more soluble, it can reach toxic levels inside plant cells, constituting an important target for studies. Here we aimed to verify morphological ... more

V.E. Viana; N. Marini; T. Finatto; I. Ezquer; C. Busanello; R.S.Dos Santos; C. Pegoraro; L. Colombo; C. de Oliveira
09/27/2017
Arachis; Genotype; Plant Leaves; Plant Proteins; Resveratrol; RNA, Messenger; Stilbenes; Tetraploidy; Transcriptional activation; Ultraviolet Rays

Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes ... more

P.A.S.V. Carvalho; A.C. Brasileiro; S. Leal-Bertioli; D.J. Bertioli; J.P. Silva; T.S. Agostini-Costa; M.A. Gimenes
12/02/2016
Alcohol Oxidoreductases; Anthocyanins; Cloning, Molecular; Flavonols; Fruit; Gene Expression Regulation, Plant; Glucosyltransferases; Organ Specificity; Plant Proteins; Transcription factors; Vitis

MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and ... more

T.Q. Niu; Z.D. Gao; P.F. Zhang; X.J. Zhang; M.Y. Gao; W. Ji; W.X. Fan; P.F. Wen
2016 Dec 19
Cloning, Molecular; Cytokinins; Gene Expression Regulation, Plant; Lactones; Nicotiana; Plant Proteins; Transcription factors

Axillary branching is controlled by a very complex mechanism involving various endogenous and environmental factors. Previous studies have shown that Tb1/BRC1 is the point of integration in the network of molecular mechanisms regulating axillary branching in plants. In this study, we cloned the Tb1/BRC1 ortholog, NtBRC1, from Nicotiana tabacum and functionally analyzed its role in the control ... more

Y.L. Gao; Z.B. Song; W.Z. Li; F.C. Jiao; R. Wang; C.J. Huang; Y.P. Li; B.W. Wang
2/16/2017
Brassica napus; Chloroplasts; Chromosome Mapping; Chromosomes, Plant; Cloning, Molecular; Multigene Family; Photosynthesis; Phylogeny; Plant Proteins; Transcription factors

Photosynthesis is the process by which dry matter accumulates, which affects rapeseed yield. In this study, we identified GOLDEN2-LIKE1 (GLK1), located on chromosome A07 and 59.2 kb away from the single nucleotide polymorphism marker SNP16353A07, which encodes a transcription factor associated with the rate of photosynthesis in leaves. We then identified 96 GLK1 family members from 53 species ... more

Y.L. Pan; Y. Pan; C.M. Qu; C.G. Su; J.H. Li; X.G. Zhang
08/31/2017
Amino Acid Motifs; Cloning, Molecular; Droughts; Hordeum; Plant Proteins; Protein domains; Qa-SNARE Proteins; Stress, Physiological

Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, "Himalaya 10"; its expression was evaluated during drought stress and rehydration via real-time PCR. The cloned HbSYR1 ... more

Q.J. Xu; Y.L. Wang; Z.X. Wei; H.J. Yuan; X.Q. Zeng; N. Tashi
3/16/2017
Adaptation, Physiological; Copper; Gene Expression Regulation, Plant; Metallothionein; Plant Leaves; Plant Proteins; Prosopis; Time Factors

For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous to the MT type 2 (isoform 1), Mt2, and Mt3 sequences of other plant ... more

C. Michel-Lopez; O. Zapata-Pérez; D. González-Mendoza; O. Grimaldo-Juarez; C. Ceceña-Duran; O. Tzintzun-Camacho
12/19/2016
Animals; Astragalus propinquus; Biomarkers; Bone Density; Disease Models, Animal; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; Female; Humans; Mice; Osteoporosis, Postmenopausal; Ovariectomy; Polysaccharides; Random Allocation; Signal transduction

Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on ... more

J. Huo; X. Sun
1/23/2017
Animals; Biomarkers; Cell line; G-Protein-Coupled Receptor Kinase 2; Humans; Mechanical Phenomena; Mice; Osteoblasts; Phosphorylation; Receptors, Purinergic P2Y2; Signal transduction

Bone desensitization after mechanical loading is essential for bone to adapt to its mechanical environment. However, the desensitization mechanism is unknown. Previous studies suggest that G protein-coupled receptors (GPCRs), including P2Y and parathyroid hormone receptors, play important roles in osteoblast mechanobiology. Thus, for the present research, we examined the role of G protein- ... more

Y. Xing; Y. Gu; X. Shan; L. Wang; J. You

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