Research Article

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1/23/2017
Gene expression; Genes, Plant; Genetic Vectors; Ginsenosides; Panax; Plants, Genetically Modified; Transformation, Genetic

Panax japonicus C.A. Meyer, a perennial herb belonging to the Araliaceae ginseng genus, is one of the seven rare and endangered Chinese medical herbs. By cloning the SS segment, the expression vectors pCXSN-PjSS and pCXSN-antiPjSS were constructed and introduced into Agrobactria LBA4404, which is used for engineering bacteria. Polymerase chain reaction (PCR) showed that the PjSS, antiPjSS, and ... more

L. Zhang; T.T. Wang
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Actins; Aquaporins; Cold-Shock Response; Cyclophilins; Eukaryotic Initiation Factor-4A; Genes, Plant; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Oryza; Plant Proteins; Real-time polymerase chain reaction; Reference Standards; Tubulin; Ubiquitins

Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pathways and cellular compartments, resulting in general or specific ... more

P.A. Auler; L.C. Benitez; M.N. do Amaral; I.L. Vighi; G.S. Rodrigues; L.C. da Maia; E.J.B. Braga
08/31/2017
Ascomycota; Chromosome Mapping; Chromosomes, Plant; Cucumis; Genes, Plant; Genetic Loci; Microsatellite Repeats; Plant Immunity; Plant Stems

Powdery mildew (PM) of cucumber (Cucumis sativus), caused by Podosphaera xanthii, is a major foliar disease worldwide and resistance is one of the main objectives in cucumber breeding programs. The resistance to PM in cucumber stem is important to the resistance for the whole plant. In this study, genetic analysis and gene mapping were implemented with cucumber inbred lines NCG-122 (with ... more

P.N. Liu; H. Miao; H.W. Lu; J.Y. Cui; G.L. Tian; T.C. Wehner; X.F. Gu; S.P. Zhang
1/23/2017
Computational biology; Gene expression profiling; Humans; Models, Statistical; Reproducibility of Results

Microarray gene expression technology provides a systematic approach to patient classification. However, microarray data pose a great computational challenge owing to their large dimensionality, small sample sizes, and potential correlations among genes. A recent study has shown that gene-gene correlations have a positive effect on the accuracy of classification models, in contrast to some ... more

M. Pan; J. Zhang
1/23/2017
Biomarkers, Tumor; Colonic Neoplasms; Humans; Models, Theoretical; Reproducibility of Results; Support vector machine

We aimed to evaluate the specificity of 12 tumor markers related to colon carcinoma and identify the most sensitive index. Bhattacharyya distance was used to evaluate the index. Then, different index combinations were used to establish a support vector machine (SVM) diagnosis model of malignant colon carcinoma. The accuracy of the model was checked. High accuracy was assumed to indicate the ... more

W.Y. Yang; G. Shi; L.P. Wu; S.T. Wei; Y.N. Huang; L.X. Tan; R.Z. Yang; C.X. Yan; E.T. Guo; H.Y. Wang; J.Z. Tong; Y. Dong; D.Z. Han
2/23/2017
Algorithms; Amino acid sequence; Amino acids; Animals; binding sites; Computational biology; Databases, Protein; Humans; Hydrophobic and Hydrophilic Interactions; Models, Theoretical; Neural Networks, Computer; Phosphorylation; Proteins; Reproducibility of Results; Support vector machine

Phosphorylation is an important part of post-translational modifications of proteins, and is essential for many biological activities. Phosphorylation and dephosphorylation can regulate signal transduction, gene expression, and cell cycle regulation in many cellular processes. Phosphorylation is extremely important for both basic research and drug discovery to rapidly and correctly identify ... more

R.Z. Han; D. Wang; Y.H. Chen; L.K. Dong; Y.L. Fan
2/08/2017
Crosses, Genetic; Hybridization, Genetic; Manihot

Two cassava escapes where collected from cultivated fields near natural habitat in Bolivia. They are described morphologically and analyzed cytogenetically in this study. It is suggested that they are the product of backcrosses of cassava interspecific hybrids with the cultigen itself, and that selective conditions have developed in which certain forms of cassava segregates have adapted to ... more

N.M.A. Nassar; M. Mendonza
09/21/2017
Animals; Evolution, Molecular; Hybridization, Genetic; Lepidoptera; Polymorphism, Genetic

Helicoverpa armigera is the most significant pest of agriculture in Asia, Europe, Africa, and Australasia, causing damage to crops greater than US$2 billion annually and until 2013 it was not detected in Brazil. Helicoverpa zea is restricted to the American continent and is important to corn and a secondary pest of cotton and tomatoes. The wide range of crops exploited by H. armigera (mainly ... more

H.M. Lopes; C.S. Bastos; L.S. Boiteux; J. Foresti; F.A. Suinaga
06/29/2017
Actins; Capsicum; Colletotrichum; Fungal Proteins; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Microsatellite Repeats; Phylogeny; Polymorphism, Genetic; Superoxide dismutase

Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular ... more

C.V.S. de Oliveira; K.S. Matos; D.M.C. de Albuquerque; R.E. Hanada; G.F. da Silva
07/06/2017
Capsicum; Fruit; Microsatellite Repeats; Polymorphism, Genetic; Quantitative Trait, Heritable; Seed Bank

Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - ... more

S.I.C. Carvalho; L.B. Bianchetti; C.F. Ragassi; C.S.C. Ribeiro; F.J.B. Reifschneider; G.S.C. Buso; F.G. Faleiro

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