Research Article

Related GMR Articles

Animals; Brazil; Cattle; Cattle Diseases; Coronavirus Infections; Coronavirus, Bovine; Dysentery; Evolution, Molecular; Feces; Membrane Glycoproteins; Phylogeny; Sequence Analysis, DNA; Viral Envelope Proteins; Viral Tropism

Bovine coronavirus (BCoV) is a pathogen related to enteric and respiratory diseases in cattle worldwide. Enteric (BECoV) strains of BCoV are predominant in South America, and genetic investigations have been conducted to identify its relationship with isolates of respiratory origin (BRCoV). In this study, we used a BRCoV strain (BR-UEL11) derived from an outbreak of respiratory disease in ... more

E.A. Beuttemmuller; A.F. Alfieri; S.A. Headley; A.A. Alfieri
Amylases; Bacillus; Bacterial Proteins; Industrial Microbiology; Polymorphism, Genetic; RNA, Ribosomal, 16S; Soil Microbiology

The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyze the genetic diversity ... more

A.R.E.O. Xavier; E.R. Lima; A.M.E. Oliveira; L. Cardoso; J. Santos; C.H.C. Cangussu; L.N. Leite; M.C.L. Quirino; I.G.C. Júnior; D.A. Oliveira; M.A.S. Xavier
Bacterial diversity; Endophytes; Mexican husk tomato plants; Rhizosphere

Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and se­quence homology comparison of the 16S rDNA genes. Sixteen opera­tional taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacte­ria, Betaproteobacteria, Actinobacteria, and Bacilli. The ... more

H.A. Marquez-Santacruz; R. Hernandez-Leon; M.C. Orozco-Mosqueda; I. Velazquez-Sepulveda; G. Santoyo
Antagonism; ARDRA; Diversity; Endophytes; Fungal inhibition; Grape

We investigated the diversity of endophytic fungi found on grape (Vitis labrusca cv. Niagara Rosada) leaves collected from Salesópolis, SP, Brazil. The fungi were isolated and characterized by amplified ribosomal DNA restriction analysis, followed by sequencing of the ITS1-5.8S-ITS2 rDNA. In addition, the ability of these endophytic fungi to inhibit the grapevine pathogen ... more

M.C.P. Brum; W.L. Araújo; C.S. Maki; J.L. Azevedo
Endophytes; Meliaceae; Molecular identification; rDNA sequencing; Trichilia

Various types of organisms, mainly fungi and bacteria, live within vegetal organs and tissues, without causing damage to the plant. These microorganisms, which are called endophytes, can be useful for biological control and plant growth promotion; bioactive compounds from these organisms may have medical and pharmaceutical applications. Trichilia elegans (Meliaceae) is a native ... more

S.A. Rhoden; A. Garcia; C.J.Rubin Filho; J.L. Azevedo; J.A. Pamphile
Endophytes; Molecular identification; Phylogeny; Piperaceae; rDNA sequencing

Tropical and subtropical plants are rich in endophytic community diversity. Endophytes, mainly fungi and bacteria, inhabit the healthy plant tissues without causing any damage to the hosts. These fungi can be useful for biological control of pathogens and plant growth promotion. Some plants of the genus Piper are hosts of endophytic microorganisms; however, there is little ... more

R.C. Orlandelli; R.N. Alberto; C.J.Rubin Filho; J.A. Pamphile
Aquatic macrophytes; Endophytes; Molecular identification; Phylogeny

Endophytic fungi live in the interior of healthy plants without causing them any damage. These fungi are of biotechnological interest; they may be used in the biological control of pests and plant diseases, and in the pharmaceutical industry. The aquatic macrophytes Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) belong to the Pontederiaceae family. The first ... more

T.T. Almeida; R.C. Orlandelli; J.L. Azevedo; J.A. Pamphile
Acerola; Antagonistic activity; Endophytes; Enzymes; Phylogenetic analysis

Acerola (Malpighia emarginata) is a shrub native to tropical and subtropical climates, which has great commercial interest due to the high vitamin C content of its fruit. However, there are no reports of the endophytic community of this plant species. The aim of this study was to verify the genetic diversity of the leaf endophytic bacterial community of two varieties (Olivier ... more

V. Specian; A.T. Costa; A.C. Felber; J.C. Polonio; J.L. Azevedo; J.A. Pamphile; V. Specian; A.T. Costa; A.C. Felber; J.C. Polonio; J.L. Azevedo; J.A. Pamphile; V. Specian; A.T. Costa; A.C. Felber; J.C. Polonio; J.L. Azevedo; J.A. Pamphile
Endophytes; Enzymes; Fungi; Phylogenetic analysis

Plants of medicinal and economic importance have been studied to investigate the presence of enzyme-producing endophytic fungi. The characterization of isolates with distinct enzyme production potential may identify suitable alternatives for specialized industry. At Universidade Estadual de Maringá Laboratory of Microbial Biotechnology, approximately 500 isolates ... more

R.N. Alberto; A.T. Costa; J.C. Polonio; M.S. Santos; S.A. Rhoden; J.L. Azevedo; J.A. Pamphile; R.N. Alberto; A.T. Costa; J.C. Polonio; M.S. Santos; S.A. Rhoden; J.L. Azevedo; J.A. Pamphile
Genetic diversity; Random amplification of polymorphic DNA; Zea mays

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were monomorphic and 92 were polymorphic. To ... more

F.S. Resh; C.A. Scapim; C.A. Mangolin; M.F.P.S. Machado; A.Tdo Amaral; H.C.C. Ramos; M. Vivas