Research Article

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3/16/2017
Adolescent; Adult; Anti-Inflammatory Agents, Non-Steroidal; Cytokines; Female; Humans; Interferon-gamma; Interleukin-10; Interleukin-4; male; Prognosis; Severity of Illness Index; Spondylitis, Ankylosing; Th1 Cells; Th2 cells; Tumor necrosis factor-alpha; Young Adult

Ankylosing spondylitis (AS), a progressive disease of the spine, manifests as peripheral arthritis with tendon and ligament inflammation that restricts activity. AS is a rheumatoid autoimmune disease although the rheumatoid factor is absent in patients with AS. It is characterized by inflammatory changes such as elevated levels of serum inflammatory factors. The roles of Th1 and Th2 cytokines ... more

J.T. Wen; D.H. Zhang; P.F. Fang; M.H. Li; R.J. Wang; S.H. Li
12/19/2016
Athletes; Cell Hypoxia; Cell proliferation; Cells, Cultured; Endothelial progenitor cells; Exercise; Gene expression; Humans; Lipoproteins; Skiing

In this study, we compared the functional properties of endothelial progenitor cells (EPCs) derived from halfpipe-snowboarding athletes who train under hyperoxic conditions with those derived from normal subjects who lived under normoxic conditions. Peripheral blood-derived EPCs were isolated from both halfpipe-snowboarding athletes and normal humans. Cellular growth dynamics, lipoprotein ... more

Y.H. Zhao; J.C. Kan; Y.F. Wang; W.J. Guan; Z.Q. Zhu
12/19/2016
Animals; Animals, Genetically Modified; Apoptosis Regulatory Proteins; Crosses, Genetic; Drosophila melanogaster; Drosophila Proteins; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Humans; male; Membrane Proteins; Neurons; Proto-Oncogene Proteins c-bcl-2; Sequence Homology, Amino Acid; Transgenes

Lifeguard is an integral transmembrane protein that modulates FasL-mediated apoptosis by interfering with the activation of caspase 8. It is evolutionarily conserved, with homologues present in plants, nematodes, zebra fish, frog, chicken, mouse, monkey, and human. The Lifeguard homologue in Drosophila, CG3814, contains the Bax inhibitor-1 family motif of unknown function. Downregulation of ... more

P.G. M'Angale; B.E. Staveley
2/08/2017
Animals; DNA damage; DNA Fragmentation; Female; Ferric Compounds; Humans; Lipid peroxidation; Lymphocytes; Metal Nanoparticles; Oxidative stress; Rats; Reactive oxygen species; Silicon Dioxide

At present, the use of nanoparticles is a controversial topic, especially when analyzing their effects in human tissues. Nanoparticles (NPs) can cause oxidative stress by increasing membrane lipids peroxidation and reactive oxygen species, and decreasing intracellular glutathione. Oxidative stress plays an important role in cell signaling and inflammatory responses. It can result in ... more

J. Jiménez-Villarreal; D.I. Rivas-Armendáriz; R.D.Arellano Pérez-Vertti; O. Calderón; R. García-Garza; N.D. Betancourt-Martínez; L.B. Serrano-Gallardo; J. Morán-Martínez
2/16/2017
C-reactive protein; Depressive Disorder; DNA-Binding Proteins; Gene Expression Regulation; Heat Shock Transcription Factors; HSP70 Heat-Shock Proteins; Humans; Interleukins; Ischemic Attack, Transient; male; Middle Aged; NF-kappa B; Stress, Psychological; Transcription factors

The aim of this study was to evaluate dysregulation of gene expression associated with the cellular stress response in a patient with a post-"warning stroke" depressive disorder confirmed by the presence of a neurophysiological neuromarker through the use of quantitative EEG and event-related potentials. The patient was tested for seven genes associated with the stress reaction: HSPA1A, HSPB1 ... more

M. Trystuła; M. Żychowska; M. Wilk-Frańczuk; J.D. Kropotov; M. Pąchalska
2/23/2017
Algorithms; Amino acid sequence; Amino acids; Animals; binding sites; Computational biology; Databases, Protein; Humans; Hydrophobic and Hydrophilic Interactions; Models, Theoretical; Neural Networks, Computer; Phosphorylation; Proteins; Reproducibility of Results; Support vector machine

Phosphorylation is an important part of post-translational modifications of proteins, and is essential for many biological activities. Phosphorylation and dephosphorylation can regulate signal transduction, gene expression, and cell cycle regulation in many cellular processes. Phosphorylation is extremely important for both basic research and drug discovery to rapidly and correctly identify ... more

R.Z. Han; D. Wang; Y.H. Chen; L.K. Dong; Y.L. Fan
3/15/2017
Adult; Biomarkers; Case-control studies; Cells, Cultured; Chelating Agents; Combined Modality Therapy; Female; Hepatolenticular Degeneration; Humans; Liver; Liver cirrhosis; male; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Penicillamine; Transforming Growth Factor beta1; Treatment outcome; Tumor necrosis factor-alpha; Young Adult

The efficacy of bone marrow mesenchymal stem cell (BMSC) on liver fibrosis in animal has been proven, but a few studies have been made in human body and few such researches in China. This study was designed to investigate the effect of BMSC treatment on hepatic fibrosis induced by hepatolenticular degeneration and the influence on serological indicators. Sixty patients with liver fibrosis ... more

D. Zhang
3/16/2017
Actins; Apoptosis; Cell proliferation; Cells, Cultured; Coculture Techniques; Down-regulation; Hepatic stellate cells; Hepatocyte growth factor; Humans; Mesenchymal stem cells; Paracrine Communication

We investigated the paracrine effects of bone marrow mesenchymal stem cells (BMSCs) on the proliferation, apoptosis, and alpha-actin-2 (ACTA2) expression of hepatic stellate cells (HSCs), and explored the possible mechanisms of hepatocyte growth factor (HGF). We established a co-culture system by culturing BMSCs on the upper layer and HSCs on the lower layer of a 6-well Transwell plate. Normal ... more

H.J. Cao; M.D. Wang; S.G. Li; L. Zhu; J.H. Zheng
4/13/2017
Animals; Bone morphogenetic protein 2; Genetic Therapy; Humans; Mandible; Osteogenesis, Distraction; Rabbits; Transforming Growth Factor beta1; Vascular Endothelial Growth Factor A

Transforming growth factor-β1 (TGF-β1) is a member of the TGF-β superfamily, and plays an important role in promoting various stages of intramembranous and endochondral bone formation. It is one of the major growth factors that influence new bone formation in the distraction gap during distraction osteogenesis (DO). The major problem of DO is the time required for the treatment. Reports show ... more

M.K. Xie; C.B. Hu; B. Zhou; G.P. Wu
4/13/2017
Animals; Gene frequency; Heterozygote; Humans; Polymorphism, Genetic; Software

The gene diversity or expected heterozygosity (H) is based on the allele frequency and is often used as a measure of genetic variability of populations. Knowing the pattern of spatial distribution of H can be useful for determining strategies of conservation and sampling of collections of individuals. In addition, it can allow one to detect genetic boundaries in a landscape. We adapted a ... more

A.R. da Silva; G. Malafaia; I.P.P. Menezes

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